Displaying GeBP Family from Rice


INFORMATION ABOUT GeBP FAMILY

Trichomes of Arabidopsis are single-celled epidermal hair that are a useful model for studying plant cell fate determination. Trichome initiation requires the activity of the GLABROUS1 (GL1) gene whose expression in epidermal and trichome cells is dependent on the presence of a 3'-cis-regulatory element. Using a one-hybrid screen, we have isolated a cDNA, which encodes for a protein, GL1 enhancer binding protein (GeBP), that binds this regulatory element in yeast and in vitro. GeBP and its three homologues in Arabidopsis share two regions: a central region with no known motifs and a C-terminal region with a putative leucine-zipper motif. We show that both regions are necessary for trans-activation in yeast. A translational fusion with the Yellow Fluorescent Protein (YFP) indicates that GeBP is a nuclear protein whose localization is restricted to, on average, 3-5 subnuclear foci that might correspond to nucleoli. Transcriptional fusion with the GUS reporter indicates that GeBP is mainly expressed in vegetative meristematic tissues and in very young leaf primordia. We looked at GeBP expression in plants mutated in or misexpressing KNAT1, a KNOX gene, expressed in the shoot apical meristem and downregulated in leaf founder cells, and found that GeBP transcript level is regulated by KNAT1 suggesting that KNAT1 is a transcriptional activator of GeBP. This regulation suggests that GeBP is acting as a repressor of leaf cell fate.


Protein Name
accepted / suggested
Gene Locus Synonym/
Gene Name
Clone in TFome Genome Browser
OsGBP1 LOC_Os01g14720 View in Browser
OsGBP3 LOC_Os01g32890 View in Browser
OsGBP4 LOC_Os02g18660 View in Browser
OsGBP5 LOC_Os02g52950 View in Browser
OsGBP6 LOC_Os02g53150 View in Browser
OsGBP7 LOC_Os03g25430 View in Browser
OsGBP8 LOC_Os03g50110 View in Browser
OsGBP9 LOC_Os06g10710 View in Browser
OsGBP11 LOC_Os07g44200 View in Browser
OsGBP12 LOC_Os08g36450 View in Browser
OsGBP13 LOC_Os09g01140 View in Browser
OsGBP14 LOC_Os09g01470 View in Browser
OsGBP16 LOC_Os09g27850 View in Browser