Displaying VOZ Family from Maize


INFORMATION ABOUT VOZ FAMILY

A 38-bp pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis thaliana V-PPase during pollen development. Here, we report the isolation and structural characterization of AtVOZ1 and AtVOZ2, novel transcription factors that bind to the 38-bp cis-acting region of A. thaliana V-PPase gene, AVP1. AtVOZ1 and AtVOZ2 show 53% amino acid sequence similarity. Homologs of AtVOZ1 and AtVOZ2 are found in various vascular plants as well as a moss, Physcomitrella patens. Promoter-?-glucuronidase reporter analysis shows that AtVOZ1 is specifically expressed in the phloem tissue and AtVOZ2 is strongly expressed in the root. In vivo transient effector-reporter analysis in A. thaliana suspension-cultured cells demonstrates that AtVOZ1 and AtVOZ2 function as transcriptional activators in the Arabidopsis cell. Two conserved regions termed Domain-A and Domain-B were identified from an alignment of AtVOZ proteins and their homologs of O. sativa and P. patens. AtVOZ2 binds as a dimer to the specific palindromic sequence, GCGTNx7ACGC, with Domain-B, which is comprised of a functional novel zinc coordinating motif and a conserved basic region. Domain-B is shown to function as both the DNA-binding and the dimerization domains of AtVOZ2. From highly the conservative nature among all identified VOZ proteins, we conclude that Domain-B is responsible for the DNA binding and dimerization of all VOZ-family proteins and designate it as the VOZ-domain.


Protein Name
accepted / suggested
Gene Locus Synonym/
Gene Name
Clone in TFome Genome Browser
ZmVOZ1 GRMZM2G111696 VOZ1 View in Browser
ZmVOZ2 GRMZM2G158717 VOZ2 pUT1681 View in Browser
ZmVOZ3 GRMZM2G094081 VOZ3 View in Browser
ZmVOZ4 GRMZM2G156016 VOZ4 pUT3226 View in Browser
ZmVOZ5 GRMZM2G449165 VOZ5 pUT6096 View in Browser