Displaying bHLH Family from Sorghum


INFORMATION ABOUT bHLH FAMILY

The basic/helix-loop-helix (bHLH) proteins are a superfamily of transcription factors that bind as dimers to specific DNA target sites and that have been wellcharacterized in nonplant eukaryotes as important regulatory components in diverse biological processes. Based on evidence that the bHLH protein PIF3 is a direct phytochrome reaction partner in the photoreceptor signaling network we have undertaken a comprehensive computational analysis of the Arabidopsis genome sequence databases to define the scope and features of the bHLH family. Using a set of criteria derived from a previously defined consensus motif we identified 147 bHLH protein-encoding genes making this one of the largest transcription factor families in Arabidopsis. Phylogenetic analysis of the bHLH domain sequences permits classification of these genes into 21 subfamilies. The evolutionary and potential functional relationships implied by this analysis are supported by other criteria including the chromosomal distribution of these genes relative to duplicated genome segments the conservation of variant exon/intron structural patterns and the predicted DNA binding activities within subfamilies. Considerable diversity in DNA binding site specificity among family members is predicted and marked divergence in protein sequence outside of the conserved bHLH domain is observed. Together with the established propensity of bHLH factors to engage in varying degrees of homodimerization and heterodimerization these observations suggest that the Arabidopsis bHLH proteins have the potential to participate in an extensive set of combinatorial interactions endowing them with the capacity to be involved in the regulation of a multiplicity of transcriptional programs. We provide evidence from yeast two-hybrid and in vitro binding assays that two related phytochrome-interacting members in the Arabidopsis family PIF3 and PIF4 can form both homodimers and heterodimers and that all three dimeric configurations can bind specifically to the G-box DNA sequence motif CACGTG. These data are consistent in principle with the operation of this combinatorial mechanism in Arabidopsis.


Protein Name
accepted / suggested
Gene Locus Synonym/
Gene Name
Clone in TFome Genome Browser
SbbHLH1 Sb01g003830 View in Browser
SbbHLH2 Sb01g004710 View in Browser
SbbHLH3 Sb01g005130 View in Browser
SbbHLH4 Sb01g006190 View in Browser
SbbHLH5 Sb01g007160 View in Browser
SbbHLH6 Sb01g008570 View in Browser
SbbHLH7 Sb01g009380 View in Browser
SbbHLH8 Sb01g009590 View in Browser
SbbHLH9 Sb01g012250 View in Browser
SbbHLH10 Sb01g012260 View in Browser
SbbHLH11 Sb01g012280 View in Browser
SbbHLH12 Sb01g013843 View in Browser
SbbHLH13 Sb01g014580 View in Browser
SbbHLH14 Sb01g026610 View in Browser
SbbHLH15 Sb01g027411 View in Browser
SbbHLH16 Sb01g027416 View in Browser
SbbHLH17 Sb01g028230 View in Browser
SbbHLH18 Sb01g033530 View in Browser
SbbHLH19 Sb01g040450 View in Browser
SbbHLH20 Sb01g042070 View in Browser
SbbHLH21 Sb01g044640 View in Browser
SbbHLH22 Sb01g045690 View in Browser
SbbHLH23 Sb01g045710 View in Browser
SbbHLH24 Sb01g047940 View in Browser
SbbHLH25 Sb01g048400 View in Browser
SbbHLH26 Sb01g049080 View in Browser
SbbHLH27 Sb02g002760 View in Browser
SbbHLH28 Sb02g004570 View in Browser
SbbHLH29 Sb02g005713 View in Browser
SbbHLH30 Sb02g006390 View in Browser
SbbHLH31 Sb02g024460 View in Browser
SbbHLH32 Sb02g024750 View in Browser
SbbHLH33 Sb02g026530 View in Browser
SbbHLH34 Sb02g026920 View in Browser
SbbHLH35 Sb02g027210 View in Browser
SbbHLH36 Sb02g027430 View in Browser
SbbHLH37 Sb02g027460 View in Browser
SbbHLH38 Sb02g028300 View in Browser
SbbHLH39 Sb02g029040 View in Browser
SbbHLH40 Sb02g029530 View in Browser
SbbHLH41 Sb02g030110 View in Browser
SbbHLH42 Sb02g030120 View in Browser
SbbHLH43 Sb02g035580 View in Browser
SbbHLH44 Sb02g035870 View in Browser
SbbHLH45 Sb02g037990 View in Browser
SbbHLH46 Sb03g000570 View in Browser
SbbHLH47 Sb03g001730 View in Browser
SbbHLH48 Sb03g002910 View in Browser
SbbHLH49 Sb03g005250 View in Browser
SbbHLH50 Sb03g006500 View in Browser
SbbHLH51 Sb03g006510 View in Browser
SbbHLH52 Sb03g008290 View in Browser
SbbHLH53 Sb03g008560 View in Browser
SbbHLH54 Sb03g008570 View in Browser
SbbHLH55 Sb03g011940 View in Browser
SbbHLH56 Sb03g025570 View in Browser
SbbHLH57 Sb03g025860 View in Browser
SbbHLH58 Sb03g028030 View in Browser
SbbHLH59 Sb03g032420 View in Browser
SbbHLH60 Sb03g036100 View in Browser
SbbHLH61 Sb03g038820 View in Browser
SbbHLH62 Sb03g042860 View in Browser
SbbHLH63 Sb03g043690 View in Browser
SbbHLH64 Sb03g044660 View in Browser
SbbHLH65 Sb03g046090 View in Browser
SbbHLH66 Sb04g001200 View in Browser
SbbHLH67 Sb04g001650 View in Browser
SbbHLH68 Sb04g007795 View in Browser
SbbHLH69 Sb04g008260 View in Browser
SbbHLH70 Sb04g009390 View in Browser
SbbHLH71 Sb04g017390 View in Browser
SbbHLH72 Sb04g022260 View in Browser
SbbHLH73 Sb04g022270 View in Browser
SbbHLH74 Sb04g023320 View in Browser
SbbHLH75 Sb04g025260 View in Browser
SbbHLH76 Sb04g027280 View in Browser
SbbHLH77 Sb04g027930 View in Browser
SbbHLH78 Sb04g029270 View in Browser
SbbHLH79 Sb04g030230 View in Browser
SbbHLH80 Sb04g030530 View in Browser
SbbHLH81 Sb04g030850 View in Browser
SbbHLH82 Sb04g031250 View in Browser
SbbHLH83 Sb04g035890 View in Browser
SbbHLH84 Sb04g036450 View in Browser
SbbHLH85 Sb05g019530 View in Browser
SbbHLH86 Sb05g023590 View in Browser
SbbHLH87 Sb05g025230 View in Browser
SbbHLH88 Sb06g012030 View in Browser
SbbHLH89 Sb06g016380 View in Browser
SbbHLH90 Sb06g016740 View in Browser
SbbHLH91 Sb06g017460 View in Browser
SbbHLH92 Sb06g020810 View in Browser
SbbHLH93 Sb06g025020 View in Browser
SbbHLH94 Sb06g025040 View in Browser
SbbHLH95 Sb06g025060 View in Browser
SbbHLH96 Sb06g026970 View in Browser
SbbHLH97 Sb06g028750 View in Browser
SbbHLH98 Sb07g000900 View in Browser
SbbHLH99 Sb07g000910 View in Browser
SbbHLH100 Sb07g002200 View in Browser
SbbHLH101 Sb07g002210 View in Browser
SbbHLH102 Sb07g002900 View in Browser
SbbHLH103 Sb07g004190 View in Browser
SbbHLH104 Sb07g021170 View in Browser
SbbHLH105 Sb07g022920 View in Browser
SbbHLH106 Sb07g023280 View in Browser
SbbHLH107 Sb07g025310 View in Browser
SbbHLH108 Sb07g025320 View in Browser
SbbHLH109 Sb07g025330 View in Browser
SbbHLH110 Sb07g025340 View in Browser
SbbHLH111 Sb07g025920 View in Browser
SbbHLH112 Sb07g026610 View in Browser
SbbHLH113 Sb07g027810 View in Browser
SbbHLH114 Sb07g028860 View in Browser
SbbHLH115 Sb07g028945 View in Browser
SbbHLH116 Sb08g019780 View in Browser
SbbHLH117 Sb08g020275 View in Browser
SbbHLH118 Sb08g020280 View in Browser
SbbHLH119 Sb08g020290 View in Browser
SbbHLH120 Sb08g020300 View in Browser
SbbHLH121 Sb08g020320 View in Browser
SbbHLH122 Sb08g020330 View in Browser
SbbHLH123 Sb08g021000 View in Browser
SbbHLH124 Sb08g022760 View in Browser
SbbHLH125 Sb09g003090 View in Browser
SbbHLH126 Sb09g004820 View in Browser
SbbHLH127 Sb09g006196 View in Browser
SbbHLH128 Sb09g006220 View in Browser
SbbHLH129 Sb09g008120 View in Browser
SbbHLH130 Sb09g022280 View in Browser
SbbHLH131 Sb09g030060 View in Browser
SbbHLH132 Sb09g030930 View in Browser
SbbHLH133 Sb10g004500 View in Browser
SbbHLH134 Sb10g005650 View in Browser
SbbHLH135 Sb10g006250 View in Browser
SbbHLH136 Sb10g008000 View in Browser
SbbHLH137 Sb10g020490 View in Browser
SbbHLH138 Sb10g022530 View in Browser
SbbHLH139 Sb10g030470 View in Browser
SbbHLH140 Sb0010s017130 View in Browser
SbbHLH141 Sb0012s004780 View in Browser
SbbHLH142 Sb2250s002010 View in Browser
SbbHLH143 Sb2273s002010 View in Browser