TFome Information for pUT1438

TFome Name:pUT1438
Genbank Insert ID:HQ858740
Protein Name:ZmARR1
Species: Maize
TF Family: ARR-B
Template: DV033253
Corresponding TF Gene: GRMZM2G126834
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGCGGCGGCAAAGGCGCGGGGAGGGGAGTTCCCCGTGGGCATGAAGGTGCTGGTTGTGGACGACGACCCGACGTGCCT
CGTTGTGCTCAAGAGGATGCTCCTTGAGTGCCGATATGACGTGACAACATGTCCTCAGGCTACAAGAGCACTAACTATGT
TGCGAGAGAATAGGCGTGGTTTTGATGTTATAATAAGTGATGTCCACATGCCGGATATGGACGGATTCAGGCTACTTGAA
CTTGTAGGCCTTGAGATGGACCTTCCAGTTATCATGATGTCTGCTGATTCAAGAACGGATATTGTAATGAACGGAATTAA
GCATGGAGCATGTGACTATTTAATAAAACCTGTCAGAATGGAGGAGCTGAAAAACATCTGGCAACATGTTATTAGGAAAA
AATTTAATGAAAACAAGGACCATGAGCATTCTGGTAGCCTAGATGACACCGATCGTAACAGACCAACCAATAATGATAAT
GAATACGCTTCCTCCGCGAATGATGGAGGTGATGGCAGCTGGAAATCTCAGAGAAAGAAAAGAGAGAAAGAAGATGATGA
AACTGACCTCGAAAATGGTGATCCTTCTTCTACATCAAAGAAACCAAGAGTTGTTTGGTCAGTTGAGCTTCATCAACAAT
TTGTGAATGCAGTCAATCACCTCGGGATAGACAAAGCTGTCCCAAAGAAAATTTTGGAATTGATGAATGTCCCAGGCTTA
ACCAGGGAAAATGTTGCCAGCCATTTGCAGAAATTCAGACTCTACCTGAAGAGAATTGCTCAGCATCATGCAGGAATACC
TCATCCATTTGTTGCGCCTGTATCTAGTGCTAACGTTGCTCCGTTAGGAGGACTGGAATTCCAAGCTTTGGCTGCTTCTG
GTCAGATCCCTCCTCAAGCTCTGGCTGCTTTGCAGGATGAACTCCTTGGTCGACCTACAAGCAGTTTGGCGTTGCCTGGA
AGGGACCAGTCATCTTTGCGAGTGGCTGCAATCAAAGGAAACAAGCACCATGAAGAAAGAGAAATAGCATTTGGTCAACC
CATATACAAGTGTCAGAATAATGCATATGGTGCATTCCCTCAAAACAGCCCAGCAGTTGGAGGATTGCAACCTTTTGCAG
CTTGGCCCAATAACAAAGTTGGTATGACTGATTCAACAAGCACATTGGGAAATGTGGGCAATTCTCAAAATAGCAATATG
CTATTGCATGAATTGCAGCAACAGCCAGACACCTTGCTGTTAGGAACCCTTCACAATATTGATGCCAAACCTTCTGGTGT
AGTTATGCCATCTCAGTCGTTAAATACATTCCCGGCTAGTGAGGGTATCTCACCTAATCAAAATCCCTTGATTATACCAT
CTCAACCCCCAAGTTTTGTGTCATCAATTCCTCCATCCATGAAACATGAATCTCTTCTTGGATTACCTTCAACGTCAACC
AGTCTGTTGGGCGGGCTTGATATGGTTAATCAAGCTTCAACAAGTCAGGCTTTGATTAGTAGCCATGGAACAAATCTTCC
TGGTCTCATGAACCGTAGCTCAAATGCAATCCCTTCACCAGGAATTAGTAATTTTCAAAGTGGAAATATTCATTATGTTG
TTAATCAGAACGCTATGGGAGTTAGCTCTAAGCCACCAGGTGTTCTAAAGACCGAGAGCACTGACTCACTGAGTTGTAGT
TATGGCTATATTGGTGGTAGCACCAGTGTGGACTCTGGCTTGTTCTCTTCTCAGTCCAAAAATCCACAGTATGATCTACT
GCAGAATCAAAATGATGTTAACGGCAGCTGGTCGCCTTCACAAGATTTTGATAGTTTTGGAAATTCTCTTGGGCAAGGCC
ACCCTGGTACCACTTCATCTAACTTCCAGAGTTCCGCCCTTGGGAAGTTGCCTGACCAAGGACGAGGGAGAAATCATGGG
TTTGTCGGGAAAGGCACTTGCATTCCAAGCCGCTTTGCAGTGGATGAGGTTGAATCTCCAACTAACAACTTGAGCCACAG
CATTGGAAACAGTGGAGACATAGTGAACCCCGACATATTTGGATTTAGTGGACATATG
TFome clones do not contain stop codon at 3'end.
Translation: MAAAKARGGEFPVGMKVLVVDDDPTCLVVLKRMLLECRYDVTTCPQATRALTMLRENRRGFDVIISDVHMPDMDGFRLLE
LVGLEMDLPVIMMSADSRTDIVMNGIKHGACDYLIKPVRMEELKNIWQHVIRKKFNENKDHEHSGSLDDTDRNRPTNNDN
EYASSANDGGDGSWKSQRKKREKEDDETDLENGDPSSTSKKPRVVWSVELHQQFVNAVNHLGIDKAVPKKILELMNVPGL
TRENVASHLQKFRLYLKRIAQHHAGIPHPFVAPVSSANVAPLGGLEFQALAASGQIPPQALAALQDELLGRPTSSLALPG
RDQSSLRVAAIKGNKHHEEREIAFGQPIYKCQNNAYGAFPQNSPAVGGLQPFAAWPNNKVGMTDSTSTLGNVGNSQNSNM
LLHELQQQPDTLLLGTLHNIDAKPSGVVMPSQSLNTFPASEGISPNQNPLIIPSQPPSFVSSIPPSMKHESLLGLPSTST
SLLGGLDMVNQASTSQALISSHGTNLPGLMNRSSNAIPSPGISNFQSGNIHYVVNQNAMGVSSKPPGVLKTESTDSLSCS
YGYIGGSTSVDSGLFSSQSKNPQYDLLQNQNDVNGSWSPSQDFDSFGNSLGQGHPGTTSSNFQSSALGKLPDQGRGRNHG
FVGKGTCIPSRFAVDEVESPTNNLSHSIGNSGDIVNPDIFGFSGHM
Request Information: This plasmid is available through Addgene. It can be ordered at this URL.
Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Dr. John Gray
Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606
jgray5@utnet.utoledo.edu
 
Plasmid Map