TFome Information for pUT1497

TFome Name:pUT1497
Genbank Insert ID:HQ858755
Protein Name:ZmCADR11
Species: Maize
TF Family: CCAAT-DR1
Template: EB705687
Corresponding TF Gene: GRMZM2G146286
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B111 UP 3' Primer Name: B111 DN
5' Primer Sequence: CAC CAT GGA CCC GAT GGA C 3' Primer Sequence: ACTGTCAATGCCAGGTCCG
5' Primer Tm (º C): 58.2 3' Primer Tm (º C): 57.7
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 sec melt, 56C 30 sec anneal, 72C 45 sec extend, 35 cycles; Step 3, 72C 7 min extend 1 cycle; Step 4, 4C hold. 1ul Phusion (New England Biolabs), HF buffer (NEB), MgCl2 1.5 mM final concentration, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGACCCGATGGACATCGTCGGCAAGTCCAAGGAGGACGTCTCCCTCCCCAAATCAACAATGTTTAAGATAATTAAGGA
GATGCTTCCTCCTGATGTACGAGTGGCAAGAGATGCACAGGATCTTCTTGTTGAATGCTGTGTAGAGTTCATCAATCTCC
TTTCGTCTGAATCGAATGAAGTGTGCAGCAGAGAAGAGAAAAAAACGATTGCTCCTGAGCATGTTATCAAGGCTCTAAGT
GATCTTGGCTTCAGAGAGTACATTGAGGAGGTTTATGCTGCATATGAACAGCACAAACTTGATACTTTGGATTCTCCAAA
AGCTGGCAAATTCACTGGGATTGAGATGACAGAGGAAGAAGCTGTTGCTGAGCAACAAAGGATGTTTGCTGAGGCTCGAG
CAAGGATGAATAATGGAGCCCCCAAACCAAAGGAGACAGAACAAGAGCCGCCACAGCAACCACAAGCACAACCCCAACTT
CAGCTGCATACTGAACCACAGCAACCCGTGCAATCTCAAGTGCAGTTGCATTCGCCAACACAGCATTCCCTCCAACCTCA
AGTGCAGCTGCACCCCCAGCCACAACAGCTCCCACAAGTGCAGGTGCACTCTCAGACGCAGCTGCACCCTCAGCCACAGC
AGCCCCAAGTGCAGGTCCATCCGCAGCTGCAGCAACTCCCACAATTACAGGCCCACTCGCAGCCACCGCAACCCCAAGTG
CAAATCCACCCACAGCCGCAGCAGCCCCCACAAGTGCAGTTGCAGTCATCAGTCCAGCAGACCTCCCAACCCCAACCTCA
GGTACATCTCTACAATCACAGGGGGGGCTCACAAGCGCAGCTGCAGCCCCAACTGCCAGGTCAGCTGCAGACACAGGGGC
AAACCGGACCTGGCATTGACAGT
TFome clones do not contain stop codon at 3'end.
Translation: MDPMDIVGKSKEDVSLPKSTMFKIIKEMLPPDVRVARDAQDLLVECCVEFINLLSSESNEVCSREEKKTIAPEHVIKALS
DLGFREYIEEVYAAYEQHKLDTLDSPKAGKFTGIEMTEEEAVAEQQRMFAEARARMNNGAPKPKETEQEPPQQPQAQPQL
QLHTEPQQPVQSQVQLHSPTQHSLQPQVQLHPQPQQLPQVQVHSQTQLHPQPQQPQVQVHPQLQQLPQLQAHSQPPQPQV
QIHPQPQQPPQVQLQSSVQQTSQPQPQVHLYNHRGGSQAQLQPQLPGQLQTQGQTGPGIDS
Request Information: This plasmid is available through Addgene. It can be ordered at this URL.
Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Dr. John Gray
Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606
jgray5@utnet.utoledo.edu
 
Plasmid Map