TFome Information for pUT1500

TFome Name:pUT1500
Genbank Insert ID:HQ858758
Protein Name:ZmALF8
Species: Maize
TF Family: Alfin-like
Template: DV168421
Corresponding TF Gene: GRMZM2G172001
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: B97 UP 3' Primer Name: B97 DN
5' Primer Sequence: CAC CAT GAA CGG CGG GGG C 3' Primer Sequence: TGCCCTGGCCCGCTTGTT
5' Primer Tm (º C): 64.7 3' Primer Tm (º C): 63.6
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 sec melt, 64C 30 sec anneal, 72C 45 sec extend, 35 cycles; Step 3, 72C 7 min extend 1 cycle; Step 4, 4C hold. 1ul Phusion (New England Biolabs), HF buffer (NEB), MgCl2 1.5 mM final concentration, template 40pg/ul final concentration.
Nucleotide Sequence: ATGAACGGCGGGGGCTCTGGGCTGGCGCCCAACGCCGCGCACACCGCGGACGAGGTCTTCCGCGACTACAAAGGCCGCCG
CGCCGGCATGATCAAGGCCCTCACCACTGATGTGGAGAGGTTCTTCAAACTCTGTGACCCTGAAAAGGAGAACTTGTGCC
TTTATGGCTATCCCGATGAGACGTGGGAGGTGACCTTGCCAGCTGAGGAAGTTCCCCCTGAGATCCCTGAACCAGCATTA
GGAATCAACTTTGCTAGGGATGGCATGAATGAGAAAGACTGGCTGGCGCTAGTTGCAGTCCACAGTGATTCCTGGTTACT
GTCTGTTGCATTCTACTTTGGTGCACGCTTTGGATTTGACAGAGAAACTAGGAGGCGACTCTTTAGCCTGATAAACAACA
TGCCCACGATATTTGAGGTAGTGACAGGAGCAGCCAAGAAGCAGGCGAAGGAGAAGACACCTAATAGTAGCAGCAAGAGC
AACAGGCCTAGTTCAAAAGTGTCAAGAGCAGAGTCTCGTTCAAAGGCTAAGGTCCCCCAAGACGAAGAAGAGAGCGGTGA
TGACGATGAGGATGAGGAGGCAGACGAGCACAACAATACCTTGTGTGGAACTTGCGGAACTAACGACAGCAAGGACCAGT
TCTGGATCTGTTGTGATAACTGCGAGAAGTGGTACCATGGGAAGTGTGTCAAGATCACGCCAGCTCGAGCTGAGCATATC
AAGCAGTACAAGTGCCCAGACTGCACCAACAAGCGGGCCAGGGCA
TFome clones do not contain stop codon at 3'end.
Translation: MNGGGSGLAPNAAHTADEVFRDYKGRRAGMIKALTTDVERFFKLCDPEKENLCLYGYPDETWEVTLPAEEVPPEIPEPAL
GINFARDGMNEKDWLALVAVHSDSWLLSVAFYFGARFGFDRETRRRLFSLINNMPTIFEVVTGAAKKQAKEKTPNSSSKS
NRPSSKVSRAESRSKAKVPQDEEESGDDDEDEEADEHNNTLCGTCGTNDSKDQFWICCDNCEKWYHGKCVKITPARAEHI
KQYKCPDCTNKRARA
Request Information: This plasmid is available through Addgene. It can be ordered at this URL.
Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Dr. John Gray
Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606
jgray5@utnet.utoledo.edu
 
Plasmid Map