TFome Information for pUT1501

TFome Name:pUT1501
Genbank Insert ID:HQ858759
Protein Name:ZmARF7
Species: Maize
TF Family: ARF
Template: EE033875
Corresponding TF Gene: GRMZM2G475263
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.C442T p.(=) Present in template EST c. A1337T p. H446L . EST sequence does not cover last SNP. None present in functional domains.

5' Primer Name: C56UP 3' Primer Name: C56DN
5' Primer Sequence: CAC CAT GGC GCA AGG AGC A 3' Primer Sequence: ATA GTC CCT CTT CCC ATC CTG CC
5' Primer Tm (º C): 61.3 3' Primer Tm (º C): 60.2
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 sec melt, 60C 30 sec anneal, 72C 45 sec extend, 35 cycles; Step 3, 72C 7 min extend 1 cycle; Step 4, 4C hold. 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 1.5 mM final concentration, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGCGCAAGGAGCAGGACGGGACCCGGAGGAGCTCTTCGAGGAGCTGTGGCGCGCCTGCGCGGGGCCGCTCGTGGAGTT
GCCCCAGACCAACGAAAGGGTCTTCTACTTCCTCCAGGGTCACCTAGAACAGCTGCAAGAGCCGACGGACCCGGCGCTGC
TGGCTGAGCAGATCAAGATGTTCCAGGTGCCCAACAAGATCCTCTGCAAGGTCGTCAACGTCGAGCTCAAGGCCGAGACA
GAAACGGACGAGATGTACGCCCAGATCACTCTGCAGCCGGAACCAGATCAAGTGGATCTACCCCAATTACCTGAACCGCC
CCTGCAGGAGACGTCAAGGCCCGTGGTGCACTCCTTCTGCAAGATCCTCACACCATCCGACACCAGTACTCATGGCGGTT
TCTCCGTTCTTCGGCGTCATGCAAACGAATGCCTCCCAGCATTGGATATGTCGATGCCGACCCCGACCCAAGAGCTCATC
ACAAAGGACCTACATGGATCTGAATGGAGGTTCAAGCACATATACAGGGGCCAACCCCGTCGGCACCTTCTGACTACTGG
ATGGAGCACGTTTGTCACATCAAAGAAGCTGATTGCTGGTGACGCGTTTGTCTACCTGAGGAGCGAGACCGGACAGCAGC
GTGTGGGAGTGAGGCGCCTCGTGCAGAAGCAAAGCACAATGCCAGCATCTGTTATATCAAGCCAAAGCATGCATCTTGGG
GTGCTTGCAAGTGCATCACACGCTATTAGGACTCATTCCATTTTCCTAGTGTATTATAGACCAAGGTTAAGCCAAAGCCA
ATACATCGTCAGGCTGAACAAGTACCTGGAATCTAGTAAAATTGGATTCGATGTGGGCATGAGGTTTAAGATGAGTTTTG
AAGGGGATGACGTCCCTATAAAGAAGTTTTCTGGAACAGTTGTCGATAAAGGAGATCTTTCTCCACAATGGCAAGGTTCT
GAATGGAAGACATTGAAGGTCAAATGGGACGAAGCCACAAACTTAAATGGCCCTGAGAGGGTTTCCTCCTGGGAAATTGA
ACCATTCGATGCATCCGCACCTGCCATCACCATGCCCGTGCAACCATCAATGAAAAACAAAAGGCCTAGGGAGACAGCTG
AAGGCCTGGATATTCATGCACTGGAACCTGCTCAAGAATTCTGGCTCTCTGGAAGGCCTGAACAGCATGAGAAGACAAGC
GTAAGCTCCAGCGAACCCAAACATCAAGTCGCTTGGACAAGTGAACGCGCAGGATACAGTGCCATGAGCAGTTCAATCTG
TCAGAATTCTGCAGTAACTGGGAGCTGGTTCAAGGGCTTCAATTCCTCCGGCTCACTCCCTTCCCTGCCAGAGATCTCCC
AGAAACTGTTTCAGGTCACCAGCAACGACGCGAGGGTTCCTCCTTGGCCTGGGCTCTCCGCTTATCATGCTGACGAGCCC
TCTTCCAAGCTGTCCTGCAACACTGCTCTGTGCAGCTACCAGACCGAGGAGGTGGCTCCAAGATTTTCCAATGCCGTCGA
AGAGGAAAAGAAGGAGCCCGGCATGTTCCGTCTGTTTGGCGTCAATTTGATTAACCATGCTAGGAGTAGTGCTACCGCTG
ACAAGACGAGTGTGGGAGCAGGGGAAACATCGGCGCGAGCTGCTGGTTCTTTTGAAGACTCTGCTCAGCTATCAAGAGTG
ACGAAGGATCATACGCACATGGTGAACGGAAGCCCCCGAGAAATCCAAAGCCACCAGAGTTGCAGTGGAAGGAGTCGTAT
CAAGGTTCAAATGCATGGAAATGACGTGTGCAGAGCTGTGGATTTGGGAAATCTGGATGGGTACGAGCAGCTGATGGGTG
AGGTTGGAGAGATGTTTGAGATCAAAGACCTTGGCTCCAAAGAGAAAGAGGAATGGAAGGTTACTTTCATTAACGACGAG
AATGAGACAATGGAAGTCGGAGCTGTTCCTTGGCAGGAGTTTTGCCAGATGGTGAGGAAGATCGTGATCCATTCCATTGG
AGACAGGGGCCATATGGAGGCTTGCCCTTGCCTGGGGCAGGATGGGAAGAGGGACTAT
TFome clones do not contain stop codon at 3'end.
Translation: MAQGAGRDPEELFEELWRACAGPLVELPQTNERVFYFLQGHLEQLQEPTDPALLAEQIKMFQVPNKILCKVVNVELKAET
ETDEMYAQITLQPEPDQVDLPQLPEPPLQETSRPVVHSFCKILTPSDTSTHGGFSVLRRHANECLPALDMSMPTPTQELI
TKDLHGSEWRFKHIYRGQPRRHLLTTGWSTFVTSKKLIAGDAFVYLRSETGQQRVGVRRLVQKQSTMPASVISSQSMHLG
VLASASHAIRTHSIFLVYYRPRLSQSQYIVRLNKYLESSKIGFDVGMRFKMSFEGDDVPIKKFSGTVVDKGDLSPQWQGS
EWKTLKVKWDEATNLNGPERVSSWEIEPFDASAPAITMPVQPSMKNKRPRETAEGLDIHALEPAQEFWLSGRPEQHEKTS
VSSSEPKHQVAWTSERAGYSAMSSSICQNSAVTGSWFKGFNSSGSLPSLPEISQKLFQVTSNDARVPPWPGLSAYHADEP
SSKLSCNTALCSYQTEEVAPRFSNAVEEEKKEPGMFRLFGVNLINHARSSATADKTSVGAGETSARAAGSFEDSAQLSRV
TKDHTHMVNGSPREIQSHQSCSGRSRIKVQMHGNDVCRAVDLGNLDGYEQLMGEVGEMFEIKDLGSKEKEEWKVTFINDE
NETMEVGAVPWQEFCQMVRKIVIHSIGDRGHMEACPCLGQDGKRDY
Request Information: This plasmid is available through Addgene. It can be ordered at this URL.
Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Dr. John Gray
Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606
jgray5@utnet.utoledo.edu
 
Plasmid Map