TFome Information for pUT1801

TFome Name:pUT1801
Genbank Insert ID:HQ858787
Protein Name:ZmbHLH166
Species: Maize
TF Family: bHLH
Template: DV529053
Corresponding TF Gene: GRMZM2G114444
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: B195UP 3' Primer Name: B195DN
5' Primer Sequence: caccATGTCTTCGTCGGGGC 3' Primer Sequence: TTCATCAATAGCACTATTAGAAACATCTGGG
5' Primer Tm (º C): 60.9 3' Primer Tm (º C): 56.7
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 sec melt, 56C 35 sec anneal, 72C 40 sec extend, 35 cycles; Step 3, 72C 7 min extend 1 cycle; Step 4, 4C hold. 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 1.5 mM final concentration, template 40pg/ul final concentration.
Nucleotide Sequence: ATGTCTTCGTCGGGGCCTTTGCCGGCGACCGGAACCCACCACGGCAGTCAAAGGGCAGAGTGTAAATCCCAGGGTTCCAC
TTCTGCACGCAAGGTACAAAAGGCTGATCGTGAGAAGATGCGAAGAGATAAATTAAACGAGCAGTTCCAGGACTTGGGAA
ATGCACTTGATCCAGACCGTCCCAGGAACGATAAGGCAACTATACTTGGTGACACAATCCAGATGCTGAAAGATTTGACT
ACTCAGGTGAACAAGCTGAAAGCTGAATACACGTCACTTTCTGAAGAAGCGTGTGAGTTAACACAAGAAAAGAATGAGCT
TAGAGATGAGAAAGCTTCACTGAAATCTGAGGTTGATAATTTGAATAATCAGTATCAGCAACGAATGCGGGTGCTATACC
CATGGGTTGGAATGGAGCCTTCTGTTGTCATGGGGCCACCTCCTGCATATCCCTACCCAGTCCCAGTTCCAATACCCTCT
GGTGCTGTACCCATGCATCCGCAGTTGCAAACATACCACTTCTTCCACAGTCAAGCTTCTGGAACCATCCAAAACACATG
CATCCCTTACATGGCATATACACAACCCTGCCATCCTCCCACTGATCAGCCGTCTAACCAACTTAATACTCCAGTTGCCC
ATTCAAGTAGTCATAGGTCTAACTCTCCAGCTCAAGACTGTAGAAGCAAGTCATCTACATTGCAGCAACCAAGCTGTGGA
GTGAGAAGCAGTGATGCTGGTGATATTGCTACTGATCTGGAATTGAAGACTCCTGGTTCTTCATGTCAATCACATGCAGA
GATTGCTTACAACGATTCCTCTTCGGATTTGAAAACAAAGAAGCATTGCATAAAGCAGATTAATGATTGCACTCTCACTG
AAAGCAGTAGTTCAAGCAGATGCTCTTCCAATGGTCCCCCAGATGTTTCTAATAGTGCTATTGATGAA
TFome clones do not contain stop codon at 3'end.
Translation: MSSSGPLPATGTHHGSQRAECKSQGSTSARKVQKADREKMRRDKLNEQFQDLGNALDPDRPRNDKATILGDTIQMLKDLT
TQVNKLKAEYTSLSEEACELTQEKNELRDEKASLKSEVDNLNNQYQQRMRVLYPWVGMEPSVVMGPPPAYPYPVPVPIPS
GAVPMHPQLQTYHFFHSQASGTIQNTCIPYMAYTQPCHPPTDQPSNQLNTPVAHSSSHRSNSPAQDCRSKSSTLQQPSCG
VRSSDAGDIATDLELKTPGSSCQSHAEIAYNDSSSDLKTKKHCIKQINDCTLTESSSSSRCSSNGPPDVSNSAIDE
Request Information: This plasmid is available through Addgene. It can be ordered at this URL.
Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Dr. John Gray
Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606
jgray5@utnet.utoledo.edu
 
Plasmid Map