TFome Information for pUT1803

TFome Name:pUT1803
Genbank Insert ID:HQ858789
Protein Name:ZmBBR4
Species: Maize
TF Family: BBR/BPC
Template: EB166751
Corresponding TF Gene: GRMZM2G118690
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B204UP 3' Primer Name: B204DN
5' Primer Sequence: caccATGGACAACCTTGGGCATAGAGAAAA 3' Primer Sequence: CCGGATGGTGATGTACCGGTTCGTGCC
5' Primer Tm (º C): 62 3' Primer Tm (º C): 66.3
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 sec melt, 61C 35 sec anneal, 72C 40 sec extend, 35 cycles; Step 3, 72C 7 min extend 1 cycle; Step 4, 4C hold. 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 1.5 mM final concentration, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGACAACCTTGGGCATAGAGAAAATGGCAGGCAAAGGCCTGAGCAATATAAAGCGCTTCATACTCAGTGGATGATACC
TCAAAGGCAGCTAAAAGACCATCAAAGTATGAATCTCCTGGCACTGATGAATGAGAAGGACAGTGCCATCCGAGAAAGGG
ACCATGCTCTCGCTGAGAAGAAAGCAGCTATAGCTGAGCGAGATATGGCATTCGCCCAGCGGGATGCTGCAATGGCTGAA
CGGAATGCTGCAATAGTGGAGAGGGACAACGCCCTAGCTGCACTCGAACTCGCCCGTACAAATGGGTTTAATATGAATAG
TGGGAATGGATTTCACCAAGGACCTCCTCTCAATGGAACAAAGAACATCCATCACCATGACCATCTGTCTCATGTCCAAA
CATCCCCGCTGCAGCTGGCCAATTCTCCATATGACCATGTCAGGGAGATGCACATATCAGAAGCATACCCTATCGCAACA
GCTCCCGCAAGTGTTGGGAAGGCTAAGAAGCCAAGGAAGAGCAATTCTCAAGCTTCTCCATCGAAGAGGCCATCAGGTGT
ACTCCGAAAAACCAAGAAAGCAACCAGCGACTGGAAGAATGCGGGGACTACTGGTGGAGCAGGAGATTCAGCCCGCGCTT
CTGTGATGAAGAACGAGTGGAAGGACCAGGATCTCGGCCTGAACCAGGTGGTGTTCGACGAGTCAACCATGCCCGCGCCT
GCCTGCTCATGCACAGGGGAGCTCCACCAATGCTACAAATGGGGCAGTGGTGGGTGGCAGTCGTCTTGCTGCACCATGAA
CATGTCCATGCACCCGCTCCCGGTGATGCCCAACAGGCGCCACGCTCGCATGGGGGGAAGGAAGATGAGCGGGGGCGCGT
TCGCGAAGCTGCTCAGCCGGCTGGCAGCAGAAGGTCATGACCTCTCGACTCCGGTGGACCTCAAGGACCACTGGGCCAAG
CATGGCACGAACCGGTACATCACCATCCGG
TFome clones do not contain stop codon at 3'end.
Translation: MDNLGHRENGRQRPEQYKALHTQWMIPQRQLKDHQSMNLLALMNEKDSAIRERDHALAEKKAAIAERDMAFAQRDAAMAE
RNAAIVERDNALAALELARTNGFNMNSGNGFHQGPPLNGTKNIHHHDHLSHVQTSPLQLANSPYDHVREMHISEAYPIAT
APASVGKAKKPRKSNSQASPSKRPSGVLRKTKKATSDWKNAGTTGGAGDSARASVMKNEWKDQDLGLNQVVFDESTMPAP
ACSCTGELHQCYKWGSGGWQSSCCTMNMSMHPLPVMPNRRHARMGGRKMSGGAFAKLLSRLAAEGHDLSTPVDLKDHWAK
HGTNRYITIR
Request Information: This plasmid is available through Addgene. It can be ordered at this URL.
Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Dr. John Gray
Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606
jgray5@utnet.utoledo.edu
 
Plasmid Map