TFome Information for pUT1860

TFome Name:pUT1860
Genbank Insert ID:HQ858796
Protein Name:ZmbZIP60
Species: Maize
TF Family: bZIP
Template: EE017439
Corresponding TF Gene: GRMZM2G444748
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C95UP 3' Primer Name: C95DN
5' Primer Sequence: CAC CAT GGC TTC CTC CAG CGG 3' Primer Sequence: GCA AAG CTG GTA CTG GAA CAT GTT CTC G
5' Primer Tm (º C): 63.1 3' Primer Tm (º C): 61.3
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 sec melt, 63.4C 35 sec anneal, 72C 45 sec extend, 35 cycles; Step 3, 72C 7 min extend 1 cycle; Step 4, 4C hold. 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 1.5 mM final concentration, 3% DMSO template 40pg/ul final concentration.
Nucleotide Sequence: ATGGCTTCCTCCAGCGGGAGCGGGAGCTCGGGCTCCCTCTCGGCGGCAACGGCGGCCCTGGCCGCCGCGGCGGGCACGGA
GGAGGAGCTGCGCGCGCTCATGGAGCAGCGCCGCGCGAAGCGGATGCTGTCCAACCGCGAGTCCGCGCGGCGGTCGCGGA
TGCGCAAGCAGCGCCACCTCGACGAGCTGACCGCGCAGGCGGCGCACCTGCGCCGCGAGAACGCGCACGTGGCCACCGCG
CTCGGCCTCACCGCGCAGGGCCTCCTCGCCGTGGACGCCGAGAACGCCGTCCTCCGCACCCAGACCGCCGAGCTCGCCGC
GCGCCTCGGCTCCCTCAACGACATCCTCGCCTGCATGAACACCAACGCCGTCGGCGCCGTCGCCGTCTCCCTCACAGCCG
CCGCCGCCGCCGCCGCTTCCTCCGACCCCTGCCTCGCCTTCGACGGCGCCACCGCCCTGGACGACCTCCTCAGATCCTGC
CCCGAGAACATGTTCCAGTACCAGCTTTGC
TFome clones do not contain stop codon at 3'end.
Translation: MASSSGSGSSGSLSAATAALAAAAGTEEELRALMEQRRAKRMLSNRESARRSRMRKQRHLDELTAQAAHLRRENAHVATA
LGLTAQGLLAVDAENAVLRTQTAELAARLGSLNDILACMNTNAVGAVAVSLTAAAAAAASSDPCLAFDGATALDDLLRSC
PENMFQYQLC
Request Information: This plasmid is available through Addgene. It can be ordered at this URL.
Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Dr. John Gray
Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606
jgray5@utnet.utoledo.edu
 
Plasmid Map