TFome Information for pUT2107

TFome Name:pUT2107
Genbank Insert ID:KJ726786
Protein Name:ZmbHLH71
Species: Maize
TF Family: bHLH
Template: EC905724
Corresponding TF Gene: GRMZM2G120021
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGAGGACTCGAGCCTGTTCCTGCAGTGGGCGATGAGCACGCTGCGGCATGAGCAACAACCCGCGGCAGCCGTCAACGA
CGACTGCAGCAGCGAGGCAACGTTCCCCTCACTCCAAGCGCTCCGCGAGGCCTCGCATGCGGCCGAGATGGTCCAGGAGC
TGATCGGAGAAGCCCCAGCGAACAGCTGGAGCTCGGGCGACACCACCGACGGCAGCATAGGAGGCAACAGCAACTCGGTC
CCTGGCCCTGCTGCGGCGGCCATGGAGCACGATGTCTGGCCTGCTGCGTCGTCGAAGACCTCTCCGGCCAGGCGCGCCTT
GAGCAGGAGCAGCAGCGACACCAACCCTCCCGTGAGCTGGAACTTCAGCGCCGCAGCCTCGGCACAGCTGGCCGGCAGCG
CCGACGGCATGCTGCCGGAATTCGCCCCCAAGAGCGCCCTACCGCCGGACCAAGCGTACGGATCACCGCGGGCAAGGAGA
GCCGGCTTGAAGAGCCTGGCGGGATCCATGTCGTCGGCGGCGTACGCTCAAGACCACATCATCGCGGAGCGGAAGCGGAG
GGAGAAGATCAACCAGCGGTTCATCGAGCTCTCCACCGTGATCCCAGGCCTCAAGAAGATGGACAAGGCAACGATCCTTT
CAGACGCGACCAAGTATGTGAAAGAACTCCATGGAAAGCTCAAGGACCTGGAGGCTGGCGGCAGCAACCGGCGCAAGAGC
ATCGAGACGGTGGTGCTCGTCAAAAGGCCGTGCCTCCATGCTGCGCCCGCGCCGGACGACGATGCCTCCCCATTGTCAGC
GTCGTCAGGCACGCCGGCTGAGACGAAGACGCAGCTGCCGGAGATCGAGGCCCGGTTCGCTGAGAATAGCGTCATGGTGA
GGATCCACTGCGAGGACGGCAAGGGGGTGGCCGTGAAGGTGCTCGCCGAGGTCGAGGAGCTCCATCTAAGCATAATCCAT
GCCAACGTCCTGCCCTTCGTAGAGGGCACTCTCATCATAACCATCACGGCAAAGGTGGAGGAGGGCTTTACCGTATCTGC
GGGGGAGATCGTTGGCAGACTCAACTCTGCACTACTGCACAACAACGAGCATAATAGATGCTGCAACATCGCTGATGAG

TFome clones do not contain stop codon at 3'end.
Translation: MEDSSLFLQWAMSTLRHEQQPAAAVNDDCSSEATFPSLQALREASHAAEMVQELIGEAPANSWSSGDTTDGSIGGNSNSV
PGPAAAAMEHDVWPAASSKTSPARRALSRSSSDTNPPVSWNFSAAASAQLAGSADGMLPEFAPKSALPPDQAYGSPRARR
AGLKSLAGSMSSAAYAQDHIIAERKRREKINQRFIELSTVIPGLKKMDKATILSDATKYVKELHGKLKDLEAGGSNRRKS
IETVVLVKRPCLHAAPAPDDDASPLSASSGTPAETKTQLPEIEARFAENSVMVRIHCEDGKGVAVKVLAEVEELHLSIIH
ANVLPFVEGTLIITITAKVEEGFTVSAGEIVGRLNSALLHNNEHNRCCNIADE
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map