TFome Information for pUT2274

TFome Name:pUT2274
Genbank Insert ID:KJ726789
Protein Name:ZmbHLH36
Species: Maize
TF Family: bHLH
Template: BT035932.2
Corresponding TF Gene: GRMZM2G008691
Transcript: T03
Vector: pENTR/SD/D-TOPO
Note: c.G447A p.(=) Present in template FlcDNA.

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGCGCTGGAAGCGGTGGTGTTCCCGAAGGAGCACCTAGCGGGCACTGCCAAGGCGCGCTCGGCGTCGTCACTGGCCTG
CGGCTTCCGCACCGAGGAGGTCGAGGACAACGGCGGTGTGGTTCTGCAAGGAGAGGCCAGCGATGCGCCCGGTGCCGGCG
CGGCCACCACGTGGGAGTGCTGGGACGCGGCGCTCTGCCCGTGCTCCACTCCACCTGCTGGTGCCGTGGAGGAGTGCTGG
GACGACGTGCGGCACCGGCTCTCCGCGTCCCCTCCGCTGCCGCCGGCGCCCTCCGCCGCCGTCGCAGCGCCATCGTCCGG
GCACGGCAAGGCAGCTGCCGCGGCGCGGCGGAGACGGCGGCAACGCCCGAAGGCGGTGAAGAACACGGAGGAGGTGGAGA
GCCAGCGGCGCAACCACATTGCCGTGGAGCGCAACCGGCGGCGGCAAATGAACGAGTACCTCTCCGTGCTCCGCTCCGCC
TTGCCGCCCTCCTACCCGCAGCGGGGTGACCAGGCGTCGATCGTGGCCGGTGCCATCAACTTCGTCAAGGAGCTGGAGCA
CCTGCTGCAGTCGCTGGAGGCGCAGAAGCGGCGGCGCCAGGGGTGCACCGAGCCACCGGCGCCGGCGCCGTTCGCCGGCT
TGTTCACGTTCCCTCAGTACTCCACCGCCGCGACGGGTGTCGTCGCTGGCTCTGGTGACGGTGCTGGCTCCGGCGGCGGC
GCCTGCGCGGCGGGGGCGCGGCGGGGCGTGGCCGACATCGAGGTGGCCGTGGCGGAGAGCCACGCGAGCGTGAAGGTGGT
CACGCCGCGGCGGCCCAGGCAGCTGCTGAGGATGGTGGTGGCGCTGCAGTGCCTCGGCCTCACCGTGCTCCACCTCAACG
TGACCACCACCGCCGACCAGCTGGTCTTGTACTCTCTCAGCCTCAAGATGGAGGACCAGTGCCGGCTGTCGTCGGTCGAC
GACATCGCCGCCGCGGTGAACGACATACTCGGCAAGATCTCCGACGAGCGCGCCAGCCGTCACCTACTAGCCGCG
TFome clones do not contain stop codon at 3'end.
Translation: MALEAVVFPKEHLAGTAKARSASSLACGFRTEEVEDNGGVVLQGEASDAPGAGAATTWECWDAALCPCSTPPAGAVEECW
DDVRHRLSASPPLPPAPSAAVAAPSSGHGKAAAAARRRRRQRPKAVKNTEEVESQRRNHIAVERNRRRQMNEYLSVLRSA
LPPSYPQRGDQASIVAGAINFVKELEHLLQSLEAQKRRRQGCTEPPAPAPFAGLFTFPQYSTAATGVVAGSGDGAGSGGG
ACAAGARRGVADIEVAVAESHASVKVVTPRRPRQLLRMVVALQCLGLTVLHLNVTTTADQLVLYSLSLKMEDQCRLSSVD
DIAAAVNDILGKISDERASRHLLAA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map