TFome Information for pUT2277

TFome Name:pUT2277
Genbank Insert ID:KJ726791
Protein Name:ZmbHLH66
Species: Maize
TF Family: bHLH
Template: BT068494.1
Corresponding TF Gene: GRMZM2G163233
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGCCGCGCCGGCGGAGGACGCGCAGCAGCGAGGAGCTCAAGGCGGAGGACTTCGTCGACTCGGTGCTCAACTTCGGCGG
CGAGGAGGATGGGGAAGAGGAGAAGCAGGAGGCCGGCGGGGACGGCCAGCCGGCGGCGGAGTTCAAGTCCAAGAACCTGG
AGGCCGAGCGGAAGCGGCGCGGCAAGCTCAACCGCAACATCCTCGCGCTCAGGGCCGTGGTGCCGAACATCACCAAGATG
AGCAAGGAGTCCACCCTCTCGGACGCTATCGACCTCATCAAGAGGCTCCAGAACCAGGTCCTTGAGCTGCAGCGCCAGCT
CGCCGACCCGCCCGGAGAGGCCTGGGAGAAGCAGGGCAGCGCGTCGTGCTCTGAGTCCTTCACTGCCACGGAAAACATGC
CGTATCAGGGTCAGATCGAGCTGGTTCCTCTGGGGCCATGCAAGTACCACCTCAGGATCTTCTGCAAGAAGGCCGGCGTC
TTCACCAAGGTGCTGGAAGCACTCTGCAGCTACAACGCGCAGGTCACCAGCCTGAACACGATAACGTTCTACGGCTACGC
TGAGAGCGTCTTCACCATCGAGGTGAAAGGCGAGCAGGATGTTGTGATGGTGGAGCTAAGGAGCCTCTTATCCAACATCG
TGGAGGTCCCAAGCAAC
TFome clones do not contain stop codon at 3'end.
Translation: MPRRRRTRSSEELKAEDFVDSVLNFGGEEDGEEEKQEAGGDGQPAAEFKSKNLEAERKRRGKLNRNILALRAVVPNITKM
SKESTLSDAIDLIKRLQNQVLELQRQLADPPGEAWEKQGSASCSESFTATENMPYQGQIELVPLGPCKYHLRIFCKKAGV
FTKVLEALCSYNAQVTSLNTITFYGYAESVFTIEVKGEQDVVMVELRSLLSNIVEVPSN
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map