TFome Information for pUT3038

TFome Name:pUT3038
Genbank Insert ID:JX428444
Protein Name:ZmCA5P8
Species: Maize
TF Family: CCAAT-HAP5
Template: BT062754
Corresponding TF Gene: GRMZM2G105317
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2908up 3' Primer Name: UT-2908dn
5' Primer Sequence: caccATGAGGAAGAAGCTCGGC 3' Primer Sequence: GTCCTCATTGTCGTAGTCCTC
5' Primer Tm (º C): 49.1 3' Primer Tm (º C): 47.6
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 48°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGAGGAAGAAGCTCGGCACCCGGTTCCCCGCGGCTCGAATCAAAAAGATAATGCAAGCAGATGAGGATGTTGGAAAGAT
TGCATTAGCAGTGCCTGTTTTAGTCTCGAGGGCTCTTGAATTGTTTTTACAAGATTTAATTGACCGGACTTATGAAATTA
CTCTTCAAAGTGGAGCAAAGACACTGAATTCCTTCCACCTGAAGCAATGTGTGAAGAGGTACAGTTCTTTTGATTTCCTA
ACTGAAGTTGTCAGCAAGGTACCAGATCTTGGTGGTGCTGATTCATGTGGAGATGAAAGAGTGTTACCTAGAAGAAGAAA
GTCAAATGGCAGTGACCCAGAGAATGATGAATCAAGATCTAGTAAAATGGCTATAAGAAATGCAAATACCAGCCCCAGAG
GACGTGGGAGGGGTCGAGGCAGAGGGCGAGGTCGGCCACCAACCAAGAGAAAGGAAGTTGGTTACGTACAATTTGAAGAT
GAGAGCAGCATGTTTGCTGAACAAGGTGAAACCTTACCAGGAGAGGGAACAGTTCCAGAGATCAACAGCGGCAACGAGAT
TACGCCTCAAAGCACGCAACCTCCGCTAACAGCCCCTGCGCAAGCTACAAATTCTAAGGTGGAAGAAGCAAGCACCGATC
ATCAGTCAGATTGGCCTATGCCAGATGCCACTGGAAACATCGGTGTTGGGCCATCTGGTTTTGGACATCTGACAGTGCAG
GTTGATGAAGATGAGGACTACGACAATGAGGAC
TFome clones do not contain stop codon at 3'end.
Translation: MRKKLGTRFPAARIKKIMQADEDVGKIALAVPVLVSRALELFLQDLIDRTYEITLQSGAKTLNSFHLKQCVKRYSSFDFL
TEVVSKVPDLGGADSCGDERVLPRRRKSNGSDPENDESRSSKMAIRNANTSPRGRGRGRGRGRGRPPTKRKEVGYVQFED
ESSMFAEQGETLPGEGTVPEINSGNEITPQSTQPPLTAPAQATNSKVEEASTDHQSDWPMPDATGNIGVGPSGFGHLTVQ
VDEDEDYDNED
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map