TFome Information for pUT3053

TFome Name:pUT3053
Genbank Insert ID:JX428458
Protein Name:ZmABI36
Species: Maize
TF Family: ABI3-VP1
Template: BT061471
Corresponding TF Gene: GRMZM2G001048
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2831up 3' Primer Name: UT-2831dn
5' Primer Sequence: caccATGAGGGGCCGCAAGAGGCA 3' Primer Sequence: GTCCTTGTTGCTGGCGCGCA
5' Primer Tm (º C): 63.4 3' Primer Tm (º C): 63.8
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 63°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGAGGGGCCGCAAGAGGCAGCAGGTGAGCGCCAGCTCGGCCGCCGCGCTGGTGAAGGGGAGGGAGAGCACCAAGCAGAA
GGAGAAAGAAGCTAGGAAGGAGAAGGAGGCGAGGGAGGCCGGCAGGGAGCCGGCGGCGAAGGAGAGGCAGATGAGGCGCA
AGGCCGGGAAGGAGAAGGAGACGGAGACGGGCGGAGAGGGGAAGGAGCCAGCCAAGGAGAAGGAGGTCAGGGAAGAGGGC
AAGGAGAACGACAAGCACAAGGAGAAGGCGAAGACGAAGGCCAACGTCTGCCCGCAGTTCTTCAAGGTCTTCTTCCCCGA
GCAGTCCACGGAGCGGCTGAGAATCCCACCCATGTTCAACCAGCACCTCAAGGAGCAGCAGCCAACCGGAGCAGTTTCCC
TGAGAGGCCCGAGCGGCAACAGATGGCAGGCAGCGCTCGCTTCAGAGTCCGAGTCGGAGGCCGCGTGGTGCTTCGACCAG
GGCTGGAAGGAGTTCGTCACGGACCACTCCCTCAGGCTAGGCCACTTCCTGGTCTTCACGCGCGACGGGCCCGCCCAGTT
CTCCGTGGCGGTGTTCTCGTCGTCAGGCGTCATCGACCCGGCAGCCCTGGACGCGAGGCCTACTGCGAACGGCGGCGCGG
CCGTCAAGCTCGAAGATGGCGAAGGCGTCGGCGTCCGAGGGGACGTGGACGCAGGCGGCGACACCAGCTCCGAGGTGTCG
CTTCTGCCCGCGGAAGAGGGCGATGGAGGAGCGACAGGGAGGAGAACAGGAGCGACGAGTGGTGGCGCTGGCGGCGCCTC
TGAGATGTCACTTGTTCTTAGAGAAGAGGGCCGTGGAGTGACAGGGAAGAGAGCGAGAGCGACGACGAGTGATCTCCCGG
CAGATGCCAGTGCGCCAAAGAAGCACTCTGCCCTCGCGAAGAAAGCTGGTAAAAGGAGGCCTCAAGCTGCCACTTCAAAG
GATGTGTCGATGATTGTTCACAATCCCACCTTCAGTTTGTTAGACGAATCCAAGACTTTCAACAAGACTCAGATCAGAGA
CAAGAACGTGCCTAGGAGCGGTAAATTCCTTCTGAGGACCACCAAAGCACCAGTTGTAATATCCCAAAGGCGCCGAGTAA
CCGAAGAAGAGAAGTGCCTTGCTCTCAGAAAGGCAATGGAGTTCAAGTCGAAGCACCCTTTCACCATGCAGATAATGATC
GAGTCTTATGTCTACGTGGGATTTTTCATGAACATCGCCTGCGAGTTCGTCAGGGAGTCGCTCCCCCGCACGAGCAAGAA
GATGACGCTCTGGGACCCGATGGGGAAGCCGTGGGACGTCAGCTACGTCTACTACGGCGACCGCGCCGCCGGCTCCTTCG
GTGGCGGCTGGGGTAGGTTCGCTCTGGGGAACAACCTGGAGAAGTCCGACGTCTGCGTCTTCGAGCTCTTCGAGGAGGAC
AACATGCGGGTGCACATATACAGGGTCGTCCCGGAAATTACCCCGCTCCTGCGCGCCAGCAACAAGGAC
TFome clones do not contain stop codon at 3'end.
Translation: MRGRKRQQVSASSAAALVKGRESTKQKEKEARKEKEAREAGREPAAKERQMRRKAGKEKETETGGEGKEPAKEKEVREEG
KENDKHKEKAKTKANVCPQFFKVFFPEQSTERLRIPPMFNQHLKEQQPTGAVSLRGPSGNRWQAALASESESEAAWCFDQ
GWKEFVTDHSLRLGHFLVFTRDGPAQFSVAVFSSSGVIDPAALDARPTANGGAAVKLEDGEGVGVRGDVDAGGDTSSEVS
LLPAEEGDGGATGRRTGATSGGAGGASEMSLVLREEGRGVTGKRARATTSDLPADASAPKKHSALAKKAGKRRPQAATSK
DVSMIVHNPTFSLLDESKTFNKTQIRDKNVPRSGKFLLRTTKAPVVISQRRRVTEEEKCLALRKAMEFKSKHPFTMQIMI
ESYVYVGFFMNIACEFVRESLPRTSKKMTLWDPMGKPWDVSYVYYGDRAAGSFGGGWGRFALGNNLEKSDVCVFELFEED
NMRVHIYRVVPEITPLLRASNKD
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map