TFome Information for pUT3090

TFome Name:pUT3090
Genbank Insert ID:JX428493
Protein Name:ZmABI48
Species: Maize
TF Family: ABI3-VP1
Template: BT041782
Corresponding TF Gene: GRMZM2G111123
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2759up 3' Primer Name: UT-2759dn
5' Primer Sequence: caccATGGCGGAGGCGGTGGC 3' Primer Sequence: GTGATCACTTTCGAAGTACAAAGTTGCTC
5' Primer Tm (º C): 68.3 3' Primer Tm (º C): 58.9
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGCGGAGGCGGTGGCGGTGGCGTACGAGGAGCAACGGCGGAGGCAGATAGAAATGAACAAGCGGAAGCTGGAGGAGCT
GCAGCTCCACCACCTCTCCGCCGCCGTCAGAGAGGCCGCCGCCGCTGCCAAACCCTCGTCGGCCAAGAAGCGGAAGGCGC
CGGTGCCGCGGGACGCCGCCACGGAGCCGCCAAGGCGGTCCGACCGACTCGCCAACCTCCCTGAGAAGCCAATGTACCGC
GAGGTCAGGGTGAACTTGCCATTTCCGGAGGGCAGCAAGTCCAATGCGCGGGTGCCGCTGGACGCTGCCAACTCCGTGGT
GGTGGAGCCACTACGGTGGTCCGACCGACTCGCCAACCTCCCCAAGAAGCCCATGTACCACGAGGATTCGGACGAGGAGA
GGAGCTACGCCATCTCTAAGGCTGAGGAGCTGGTGCAAGAGCTGGGCTCCAGCTTCCCCATCTTCACCCAGCCCATGACA
CTGTCCCATGTCTCCGGAGGCTTCTGGCTGGGCCTCCCGAAGCATTTCTGCCGGGAGCACCTGCCTAAGAGTGATGAGAC
GATCACCTTGGTGGATGAGGAGGATGACGAGTCAGACACGTTCTACCTTGCAATGCAGGCTGGCCTCAGCACTGGATGGA
AAGAGTTCGCCATCCAGCACAAGCTGCTTGATGGAGATTGCTTGGTCTTCCAACTGATTGAGCGGACAAAGTTCAAGGTC
TACATAATTCGAGCAACTTTGTACTTCGAAAGTGATCAC
TFome clones do not contain stop codon at 3'end.
Translation: MAEAVAVAYEEQRRRQIEMNKRKLEELQLHHLSAAVREAAAAAKPSSAKKRKAPVPRDAATEPPRRSDRLANLPEKPMYR
EVRVNLPFPEGSKSNARVPLDAANSVVVEPLRWSDRLANLPKKPMYHEDSDEERSYAISKAEELVQELGSSFPIFTQPMT
LSHVSGGFWLGLPKHFCREHLPKSDETITLVDEEDDESDTFYLAMQAGLSTGWKEFAIQHKLLDGDCLVFQLIERTKFKV
YIIRATLYFESDH
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map