TFome Information for pUT3096

TFome Name:pUT3096
Genbank Insert ID:JX428498
Protein Name:ZmARF32
Species: Maize
TF Family: ARF
Template: BT033698.1
Corresponding TF Gene: GRMZM2G181254
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2970up 3' Primer Name: UT-2970dn
5' Primer Sequence: caccATGTATAACGTGTATATGTATCCTTCCCTCC 3' Primer Sequence: CCTTCGTGCTTCGAGTTGCGT
5' Primer Tm (º C): 57.2 3' Primer Tm (º C): 60.3
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGTATAACGTGTATATGTATCCTTCCCTCCTAATCTTCCGCCCCGAACAGCATTCTCCTCCTCCCTCCCTCCCTCCCTC
CCTCCCTCTCTCCGCTTCGCCAAACCCTGGTACTCAGAAGACAATGGCGACGCAGCAACCGCTTCCCCCCGCCGACTGCG
TCGGCGATAACACCGTCGACCGCGACGTCTGGCTGGCCTGCGCCGCACCGTTTTCCCGCATCCCCACCGTCGGCGACGAG
GTCGACTACTTCCCCGACGGCCACATCGAGCAGCACCTGTCCGCGGCGCCGCAGCCGCTCCCTGCGCAGCACCGCTTCCA
CTGCACCGTCACCGACGTGAGCCTCGGCGTGGACGACAAGACGGACGAGGTGTTCGCCAAAATCTCCCTCAGACCACGGC
CAGGCCGCGCCGCCGCTCCTCCTCCGGGCCCCGGCGGCAGCAGCAACAGCCCCGCCCCCAGCCCCCCACAGAAGCTCAGA
TACTTCACTAAGGAGCTCTCGCAAACCGACGTCTACGCAAGGTTCCGCATCCCGCTGGACAACGAGCACGTCTTACCCAT
ACCCATGGTCGACACGGACGGCGTGCAGCGCCAGGACGTCGTCATGCGCGACACCAGTGGCAAATCGTGGAGGTTCTCCA
AAACCTACAGCGTCAATCCGAGCAAGCAACACTCGCTCACCACCGGGTGGTTGGATTTCGCCAAGGCCAAGCGGCTCGCC
GCGGGGGACAAGATCGTCTTCATGCGCCGCCCCAACGGCGACCTCATCGTCGGCGTGCGGCGCCTCGACGTGCCCAGGTA
CCCGCTGTTCGACTTCCAAGGCCCGGACCCGGATCAGCCGGCCCAGGACGTCATGGAGGCCGTACGCCTCGCCGCCGCGG
GCAGGCCCTTCACAGTCACCTACTTCCCGAGACAGGCCGCCGTGGAGTTCATCGTCCCGCGCAGCGAGGTGGATGACGCG
CTCGCCACCAGCTGGGAACCAGGTGCGCTGGTGCGCATGGAGGTTATGGAGGACGAGAACAGGCAGTACACCATGTGGGT
CGTCGGCCGCGTCGAGGCGATCCGCCAAAACATCTGGCGAATGCTCGAGATCATCTGGGGTGTTCCGTCCCATCCTCCTC
CTCTTGCAACAATGAGATCTGTGAATGCTTGGCAAGTGTCACGCCCCTGGCCACCGGAAGGTCACCGGCGACGTGAGCCA
CACCAATTCGGCGACGAAGCGCCAGGAACACAGACGCAACTCGAAGCACGAAGG
TFome clones do not contain stop codon at 3'end.
Translation: MYNVYMYPSLLIFRPEQHSPPPSLPPSLPLSASPNPGTQKTMATQQPLPPADCVGDNTVDRDVWLACAAPFSRIPTVGDE
VDYFPDGHIEQHLSAAPQPLPAQHRFHCTVTDVSLGVDDKTDEVFAKISLRPRPGRAAAPPPGPGGSSNSPAPSPPQKLR
YFTKELSQTDVYARFRIPLDNEHVLPIPMVDTDGVQRQDVVMRDTSGKSWRFSKTYSVNPSKQHSLTTGWLDFAKAKRLA
AGDKIVFMRRPNGDLIVGVRRLDVPRYPLFDFQGPDPDQPAQDVMEAVRLAAAGRPFTVTYFPRQAAVEFIVPRSEVDDA
LATSWEPGALVRMEVMEDENRQYTMWVVGRVEAIRQNIWRMLEIIWGVPSHPPPLATMRSVNAWQVSRPWPPEGHRRREP
HQFGDEAPGTQTQLEARR
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map