TFome Information for pUT3099

TFome Name:pUT3099
Genbank Insert ID:JX428501
Protein Name:ZmARID10
Species: Maize
TF Family: ARID
Template: BT086290.1
Corresponding TF Gene: GRMZM5G883993
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2991up 3' Primer Name: UT-2991dn
5' Primer Sequence: caccATGTCGCAAGAGCAAGGTATGGAGGAGC 3' Primer Sequence: TTTTGATTGTTCAAATGGTGCACGCACA
5' Primer Tm (º C): 63.7 3' Primer Tm (º C): 60.3
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59.5C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration
Nucleotide Sequence: ATGTCGCAAGAGCAAGGTATGGAGGAGCCGCCGCCGCAGGCGGTAGGCGAGGCACAATCGGCCGCCGATGTCCCCATGAG
CGAGGCCGCAGCGGTGGAGGATGAGGATGAGGAACCCGTCATGGGGGAGGGGCAGGGCGGTGAAAGCGGGACCGCCGATG
CCGTTGATTCCGTCAAGGCTTCAGTGAAGCCCGAAGCAGAGGGCGCGGAGGAAGGGGACAGAGAGGAGCTTAATGGTGGC
CCGACGGCGGATTTGACTGACGAGGTTGTGAAATTGGAGAACGGGGATGGCCCCGTGCCGGTGGGTGGCTCCGCGGATGA
GTGCGGAGCTGGAGATGGCGGTGTTGATAAGGGCCTGGTTGGTCAGAATGAGCCTGTTGTGAACCAGCTTGTGCTGGTGC
AGGCTGAGGAGGAGCTGGCATTGTCTAAGATCTCAAACAGTTCCTTCATGTTCGATTACACCACTGGGGGTGATGACTCA
GGGACTGAGGAGGAGCAGGCTGCCTTCATGAAGGAGCTGGAGCGTTTCCACAGGGAGAAAATGTTGGAGTTCAAGCCCCC
TAAGTTTTATGGTGAAGGATTGAATTGCCTCAAATTGTGGAGACAAGTTACTGGATTGGGTGGCTATGATCAGGTGACAT
CATGCAAGCTGTGGCGTCAAGTGGGAGAGTCATTCAAACCTCCAAAGACATGTACAACTGTTTCATGGACTTTCCGTAAC
TTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACAGGAGAGTTCCAAGTAGCTTCATCTGCTCTACC
AGATCGAGTTGGTTCTGAGAGCCAGGTGGGTGGAAGCCACGTATCTGGTTCTGGAAGAGCCAGAAGAGAATCTGCAACTC
GTGCAATGCAAGGCTGGCATTCTCACCGCCTACTAGGCAATGGTGAAAATGCTGATCCTATAATTAAGGATAAAGGGCCT
ATTGTCTTAAAGAAGGATAAAACTCCTAAAAGCAGTGGTTCTGCCAAGAGGAAAAGGATACCATCCCTTGAAGATGACAT
GGTGATACCTTATAAATCCGATAAGCTACAAAATGACTCAATGGTTATTGACATGGGCCCTCCTGCTGATTGGGTGAAAA
TAAATGTTAGGAAAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAAGAGGTACATGTTCAG
TCTGACCCTGCTGGTCGTTTGATAGTTACTGGAGAGCCCGAGCAATTGGATAACCCATGGGGTGTCACTCCATTCAAGAA
GGTCATTAGGTTGCCTTCCCGCATTGATCCTCACCAAACCTCTGCAGTTGTCACCCTCCATGGGCAGCTATTTGTGCGTG
CACCATTTGAACAATCAAAA
TFome clones do not contain stop codon at 3'end.
Translation: MSQEQGMEEPPPQAVGEAQSAADVPMSEAAAVEDEDEEPVMGEGQGGESGTADAVDSVKASVKPEAEGAEEGDREELNGG
PTADLTDEVVKLENGDGPVPVGGSADECGAGDGGVDKGLVGQNEPVVNQLVLVQAEEELALSKISNSSFMFDYTTGGDDS
GTEEEQAAFMKELERFHREKMLEFKPPKFYGEGLNCLKLWRQVTGLGGYDQVTSCKLWRQVGESFKPPKTCTTVSWTFRN
FYEKALLEYEKHKIETGEFQVASSALPDRVGSESQVGGSHVSGSGRARRESATRAMQGWHSHRLLGNGENADPIIKDKGP
IVLKKDKTPKSSGSAKRKRIPSLEDDMVIPYKSDKLQNDSMVIDMGPPADWVKINVRKTKDCYEVYALVPGLLREEVHVQ
SDPAGRLIVTGEPEQLDNPWGVTPFKKVIRLPSRIDPHQTSAVVTLHGQLFVRAPFEQSK
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map