TFome Information for pUT3102

TFome Name:pUT3102
Genbank Insert ID:JX428504
Protein Name:ZmARF2
Species: Maize
TF Family: ARF
Template: BT060467
Corresponding TF Gene: GRMZM2G153233
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2735up 3' Primer Name: UT-2735dn
5' Primer Sequence: caccATGAAGGAGGTGGCCGAGGAAA 3' Primer Sequence: AGCCATGTACTCGACGAGAGGTTTCT
5' Primer Tm (º C): 60.6 3' Primer Tm (º C): 59.6
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGAAGGAGGTGGCCGAGGAAAGGTGCCTTGACCCGCAGCTGTGGCACGCGTGCGCTGGCGGCATGGTGCAGATGCCGCC
CGTGCGCTCCCGTGTCTACTACTTCCCGCAGGGTCACGCGGAGCACGCGCATGGCGGCGGGGCCGCGGACCTCGCCGGGG
CGCGAGCGCTCCCGTCGCTCGTGCTCTGCAGCGTCACCGGGGTGCGCTTCCTGGCCGATCCGGAGACCGACGAGGTCTTC
GCCAAGATCCGCCTGGTGCCGGTCGCGCCCGGCGAGGTGGAGTTCCGAGAGCCCGATGAGTTCAGCGTCGACCCCGCGGA
CGCCCGGGAGAAGCTTTCTTCCTTCGCCAAGACGCTGACGCAGTCCGACGCCAACAACGGTGGCGGCTTCTCGGTGCCGC
GCTACTGCGCCGAGACCATCTTCCCCAAGCTCGACTACCGGGCCGACCCGCCGGTGCAGACGGTGCTTGCCAAGGACGTG
CACGGGGAGGTCTGGAAGTTCCGCCACATTTACCGGGGCACGCCGCGCCGGCATTTGCTCACCACGGGATGGAGCACGTT
CGTCAACCAGAAGAAGCTCGTCGCCGGGGACTCCATCGTCTTCCTGCGCACTGAGCATGGCGAGCTGTGCGTCGGGATAC
GGCGTGTCAAGCGGGTGTCCTGTGGTGGCATGGAGTGCATGTCTGGGTGGAACGCCCCGGGGTATGGGGCCTTGTCGGCA
TTCTTGAAGGATGAGGAGGGTAAGATGATGAAGAGTCATGGTGGGTACATGAGAGGTAGGGGGAAGGTGAAGATCACGGA
TGTTGTCAACGCCGCAAGTCTAGCAGCGAGCGGACAACCATTTGAAGTTGTGTACTACCCGAGAGCTAGCACACCGGAGT
TTGTTGTGAAGGCTGCATCGGTACAGAACGCGATGAGGAACCAGTGGTGCCCGGGGATGAGGTTCAAGATGGCATTTGAG
ACAGAGGATTCGTCAAGGATCAGTTGGTTTATGGGGACGATAGCTTCTGCTCAGGTTGCTGACCCAATCAGATGGCCAAA
TTCACCGTGGAGGCTTCTTCAGGTGGCATGGGATGAACCGGACTTGCTGCAGAACGTGAAATGTGTGAACCCATGGCTCG
TGGAGATCGTATCAAGCATTCCACCGATCCACCTGGGACCATTTTCTCCACCTAGGAAGAAGCTGCGGGTGCCCCACCAT
CCGGACTTCCCGTTCGACGGCCAGCTGTTGAATCCGATTTTCCACGGCAACCCACTCGGTCCGAGCAACGGCGGCGGTGC
TCTTCGCTGTTTCTCGGACATCGCTCCTGCAGGCATACAGGGAGCCAGGCATGCTCAGTTCGGTCTACCACTAACAGACC
GCCAGCTTAACAAGCTGCACCTCGGTCTGTTCCAAGGCGGCGGTTTTAAGCGGCGGCTTGACGCTATCACTCCACCATGC
CCTATATCCAGGGGCTTTGTGATCGGCAGTGCACCGGTCGACGAGAGCGTCTCTTGCGTGCTGACGATCGGCACGCCACG
GGCCGCAGAGAGATCTGACGACAGGAAGAAACCACATCTAATGCTGTTCGGAAAGCCTATCCTTACCGAGCAGCAGATGA
GCTCTAGAGGGTCAAGGGAAACACTCTCCCCTGAGGCCACTGGGAACAGTTCCGATGGCAGTGTTCAGAAAACGGGAAAC
GTATCCGATGGCTCCGGCTCGTCGATCTGCATCGGTTCCTCGTCCCGAGGCCGCGAGGCTTCTAGGCTCGGGTTCGAATT
TGAAGCCGGGCACTGCAAGGTATTTGTGGAGTCGGAGGATGTCGGTCGCACCATCGACCTGTCCGTCTTTGGCTCGTACG
AAGAGCTGTACGGTCAGTTGGCCGACATGTTTGGAATCGAGAAGGCAGAGGTAATGAGCCACCTTTGTTACCGTGATGCT
GCTGGCGCTGTCAAGCGTACTGGCGACGAGCCGTTCTGTGACTTCATGAAAGTGGCACGGAGGCTCACCATAGTAGAGAG
CACCGAGGGGAGGCTGCAGAAACCTCTCGTCGAGTACATGGCT
TFome clones do not contain stop codon at 3'end.
Translation: MKEVAEERCLDPQLWHACAGGMVQMPPVRSRVYYFPQGHAEHAHGGGAADLAGARALPSLVLCSVTGVRFLADPETDEVF
AKIRLVPVAPGEVEFREPDEFSVDPADAREKLSSFAKTLTQSDANNGGGFSVPRYCAETIFPKLDYRADPPVQTVLAKDV
HGEVWKFRHIYRGTPRRHLLTTGWSTFVNQKKLVAGDSIVFLRTEHGELCVGIRRVKRVSCGGMECMSGWNAPGYGALSA
FLKDEEGKMMKSHGGYMRGRGKVKITDVVNAASLAASGQPFEVVYYPRASTPEFVVKAASVQNAMRNQWCPGMRFKMAFE
TEDSSRISWFMGTIASAQVADPIRWPNSPWRLLQVAWDEPDLLQNVKCVNPWLVEIVSSIPPIHLGPFSPPRKKLRVPHH
PDFPFDGQLLNPIFHGNPLGPSNGGGALRCFSDIAPAGIQGARHAQFGLPLTDRQLNKLHLGLFQGGGFKRRLDAITPPC
PISRGFVIGSAPVDESVSCVLTIGTPRAAERSDDRKKPHLMLFGKPILTEQQMSSRGSRETLSPEATGNSSDGSVQKTGN
VSDGSGSSICIGSSSRGREASRLGFEFEAGHCKVFVESEDVGRTIDLSVFGSYEELYGQLADMFGIEKAEVMSHLCYRDA
AGAVKRTGDEPFCDFMKVARRLTIVESTEGRLQKPLVEYMA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map