TFome Information for pUT3106

TFome Name:pUT3106
Genbank Insert ID:JX428508
Protein Name:ZmARF12
Species: Maize
TF Family: ARF
Template: BT069005.1
Corresponding TF Gene: GRMZM2G437460
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2816up 3' Primer Name: UT-2816dn
5' Primer Sequence: caccATGGCGGGCATCGACCTCAA 3' Primer Sequence: CATATCTGTCGCCGAGACGTCAACC
5' Primer Tm (º C): 62 3' Primer Tm (º C): 62.5
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 62°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGGCGGGCATCGACCTCAACACCGTGGAGGAGGAGGACGAGGAGGAGGCGGAGGCGCTGCCTTTGCCCGGACCCGGTGG
CCGTGGCGCCGTGTGCCTGGAGCTGTGGCACGCGTGCGCGGGCCCCGTCCCGCCGCTGCCGCGGAAGGGCAGCGCCGTCG
TGTACCTGCCGCAGGGCCACCTCGAGCACATCGGCGGCGACGCGGCGCGAGGAGCAGCAGCTTCGGCGGTGCCGCCGCAC
GTGCTCTGCCGCGTCGTCGACGTCACCCTCCACGCGGACGGCGCGACAGACGAGGTGTACGCGCGGGTGTCGCTGCTGCC
CGAGGACGAGGACGCGGAGAAGCGGGCGCAGGCGCAGGCGCGGGTTCGGGAGGACGAGGACCGCCGCGACGGCGAGGACG
GCGGCGCCATGAGGCCGCTCGCACGGACGCCGCACATGTTCTGCAAGACGCTCACGGCATCCGATACCAGCACGCACGGC
GGTTTCTCAGTGCCGCGCCGCGCCGCCGAGGACTGCTTCCCGCCGCTGGACTACAGCCAGCAGAGGCCGTCTCAGGAGCT
CGTGGCCAAGGATCTACACGGCACGGAGTGGAAGTTCCGGCATATCTATCGAGGTCAGCCACGAAGGCATCTCTTAACCA
CTGGATGGAGTGCATTTGTTAATAGGAAGAAGCTCATTTCTGGAGATGCAGTTCTCTTCCTTCGAGGCGAAGATGGAGTG
CTTCGACTGGGAGTGCGCCGAGCAGCCCAGCTAAAAATTGTAACTCCTATTCCTGCACCTCATAACCAGTGCTCAAGTAA
TAGCAATCTTGGAAACGTTGCACAAGCTGTGGCCACGAAGACTGTTTTCCACATCTACTACAATCCCAGGTTAACTCAAT
CTGAATTCATTGTACCCTATTGGAAGTTCACAAGAAGCTTCAATCAACCAATTTCTGTTGGAATGAGGTGCAGAATGCGA
TATGAAAGTGACGATGCTTCTGAAAGAAGGTGCACTGGTATAATAATTGGAAGCAGAGAAGCTGATCCTATATGGTATGG
TTCGAAATGGAAATGCTTGGTGGTTAGATGGGATGATGGTATAGAGTGCCGTTGGCCCAATAGGGTATCTCCTTGGGAGA
TTGAGCTCACAGGATCTGTTTCAGGATCTCAAATGTGCGCTCCCAGTTCAAAACGTCTGAAACCATGCCTCCCCCAAGTT
AATCCGGAGATTGTGCTTCCAAATGGAAGTGTTTCTTCAGATTTTGCGGGATCTGCCAGATTCCACAAGGTCTTGCAAGG
TCAAGAATTGTTGGGTTTCAAAACCCATGATGGTCCTGCTATTTCTGCTTCTCAGGCAACTGAAGCAAGAAATTTGCAGT
ACAGTGATGAACGGAGTAACAATAACTTAGGGATCCCAAGACTTGGTGTTAGGTCTCCAACTGGAATCCCTGGGTTTCCC
TACCATTGCTCAGGCTTTGGGGAATCTCAAAGATTCCAAAAGGTCTTGCAAGGTCAAGAAGTGTTTCATCCTTTCCGAGG
AGGATGTTTGGCTGATGGCCATATAAGAACTGCTGGCATGTATCAACCTGACGGTAGGCATGTATCTGGTGCAGCTTATA
AATGGTCTGCACCACAAGGGTATGATTTCCCACAGCCAGCAAAACCGGTTTTTCTGTTGCAAGAATCATCCCCATCATCT
GTGATGATGTTTCCACAAACTAGATCTAAGATAACTCACTTGGAATATGAGTACTCAAGACATGAGGATGGTAGACTTGA
TAGGACTGTTCCTACTCAAGACATGGGAAGAAGCAATCAAACATTATCTCTTTGGCCTCATCTTGTTTCAGGCGAAGCAA
TAGAAGAATGTACTGGAACTGTGAATATGCACTCCCCCGTCAGTGGTGCAGAGCATGAATCAAACAATGAGAGTACAGTT
GAAAATGGCTGCAAAATCTTTGGTATCTCATTGGCTGAAAAGATCCGATCATGCGATGAAGCAGACTCTTGTAGTGCAAA
ATGTAATTCTCGGCTCCAGCCTTTGAAGTCACAAATGCCAAAATCGCTAGGCAGCTGTTGGGCCACTGTTCACGAGCAAA
GGCCTGTTGTTGGCAGGGTGGTTGACGTCTCGGCGACAGATATG
TFome clones do not contain stop codon at 3'end.
Translation: MAGIDLNTVEEEDEEEAEALPLPGPGGRGAVCLELWHACAGPVPPLPRKGSAVVYLPQGHLEHIGGDAARGAAASAVPPH
VLCRVVDVTLHADGATDEVYARVSLLPEDEDAEKRAQAQARVREDEDRRDGEDGGAMRPLARTPHMFCKTLTASDTSTHG
GFSVPRRAAEDCFPPLDYSQQRPSQELVAKDLHGTEWKFRHIYRGQPRRHLLTTGWSAFVNRKKLISGDAVLFLRGEDGV
LRLGVRRAAQLKIVTPIPAPHNQCSSNSNLGNVAQAVATKTVFHIYYNPRLTQSEFIVPYWKFTRSFNQPISVGMRCRMR
YESDDASERRCTGIIIGSREADPIWYGSKWKCLVVRWDDGIECRWPNRVSPWEIELTGSVSGSQMCAPSSKRLKPCLPQV
NPEIVLPNGSVSSDFAGSARFHKVLQGQELLGFKTHDGPAISASQATEARNLQYSDERSNNNLGIPRLGVRSPTGIPGFP
YHCSGFGESQRFQKVLQGQEVFHPFRGGCLADGHIRTAGMYQPDGRHVSGAAYKWSAPQGYDFPQPAKPVFLLQESSPSS
VMMFPQTRSKITHLEYEYSRHEDGRLDRTVPTQDMGRSNQTLSLWPHLVSGEAIEECTGTVNMHSPVSGAEHESNNESTV
ENGCKIFGISLAEKIRSCDEADSCSAKCNSRLQPLKSQMPKSLGSCWATVHEQRPVVGRVVDVSATDM
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map