TFome Information for pUT3107

TFome Name:pUT3107
Genbank Insert ID:JX428509
Protein Name:ZmARF9
Species: Maize
TF Family: ARF
Template: BT055015.1
Corresponding TF Gene: GRMZM2G073750
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2842up 3' Primer Name: UT-2842dn
5' Primer Sequence: caccATGAACCTCTCACCGCCCAGGAT 3' Primer Sequence: GTAGTCCAGAGGCACCACAGGTGC
5' Primer Tm (º C): 61.6 3' Primer Tm (º C): 60.7
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 61°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGAACCTCTCACCGCCCAGGATGTCGGAGCCATCGCTTGAAAGTGACGGGGAACAAAGGTGTCTGAACTCGGAGCTATG
GCATGCATGCGCAGGGCCCCTTGTGTCCCTTCCTGTGGTTGGGAGCCGGGTCGTCTACTTTCCCCAGGGCCATAGTGAGC
AGGTTGCTGCATCAACTAATAAAGAAGTTGACGCTCAGATCCCAAACTACCCAAACCTACCTCCACAATTAATCTGCCAG
CTTCATAATGTCACAATGCATGCTGATGCAGAGACTGGAGAGGTTTATGCCCAGATGACACTGCAACCATTGAGCCCGGA
AGAGCAAAAGGAACCTTTTCTTCCAATTGAATTGGGTGCTGGTAGCAACCAGCCAACTAATTACTTCTGCAAGACGTTGA
CTGCAAGTGATACGAGTACCCATGGCGGGTTCTCTGTTCCACGCCGAGCAGCTGAGAAAGTCTTCCCCCCATTGGATTTT
TCACAACACCCTCCAGTGCAAGAGCTGGTTGCAAGAGATCTGCATGATAATGAGTGGAAATTCCGCCACATTTTTCGTGG
TCAGCCAAAAAGGCATCTACTGACGACTGGTTGGAGTGTTTTTGTAAGCGCAAAAAGACTAGTAGCAGGGGATTCTATCA
TATTTATCTGGAATGATAATAACCAGCTTCTATTGGGAATTCGTCGAGCAAATCGTCCACAGACTGTTATGCCCTCTTCC
GTGCTATCAAGCGATAGCATGCACATTGGTCTCCTTGCTGCAGCAGCTCATGCTGCAGCCACAAACAGTCGTTTCACTAT
TTTCTACAACCCCCGGGCCAGCCCTTCTGAGTTTGTGATTCCACTGGCTAAGTATGTGAAAGCTGTCTACCACACACGTG
TGTCTGTTGGAATGCGGTTTCGGATGCTTTTTGAGACAGAGGAATCAAGCGTCAGACGATACATGGGTACGATCACATGC
ATAAGTGATCTGGACTCAGAGCGTTGGCCAAATTCACACTGGCGTTCTGTGAAGGTTGGTTGGGATGAGTCTACAGCTGG
TGATAAACAGCCAAGAGTTTCACTCTGGGAGATTGAACCACTGATGGCCTTTCCGATGTACCCAACTGCCTTTCCATTAA
GGCTAAAGCGTCCGTGGGCTTCAGGGCTACCTATGTTCAATGGTGGGAGGAGTGATGAGTTCGCTCGTTATTCTTCTCTC
ATGTGGCTCCGAGATGGAAACAGAGGGGCTCAATCACTGAACTTCCAGGGACTTGGAGCATCACCATGGCTTCAACCAAG
AATAGATTATCCATTATTAGGTCTGAAGCTAGATACTTACCAGCAGATGGCTGCTGCTGCACTGGAAGAAATTAGAGCTG
GGGATCATTTGAAGCAGATATCTTCTCTCCTGCCAGTGCAACAGCCACAAAATTTGAGTGGTGGATTAGATCCTTTATAT
GGAAATCCTGTTCTGCAGCAGATGCAGTTCCAATCTCAGCAGTCATCCTTGCAAGTTGTGCAGCAAGGTTATGGTCCAAA
TGCAAGCGACTCTGGGTTCCTTCAAAATCAGCTGCAGCTGCAAAAGCAGCAGGAGCCACTACCACAGCAGCAGCAGCAAG
CTCTGCAACAACAATCGCATCAGGAAATGCAACATCACCTCTCAGCTAGCTGTCATGATATTGCTAATGTAGCTTCTGGT
GTGTCTGAGTCTGGATCTGCTTCTCAAACAGAATCTTCTTTGCTTTCTGGGTCATCGTACCAACAGATCTATGATGGAAA
CAGTGGTCCAGGTCTGCATCTGCACAACGGTTTCCACAACTGTTCAAGCCAAGAGTCCTCCAACCTTCTTAATCTCTCAA
GAAGTGGACAATTCATGGCATCAGAGGGATGGCCTTTGAAGAGGTTGGCTGTGGAATCTCTTTCTGGTCATGAGCTCCAA
CCTGTGCAGCACAAGTTTGAGAAAGTGAATCACCAAAGTAATGTGTCTCACATTTCTAGCACCTTGCCCCCACTGTCAGC
AAGAGACAGTTATAGTGCCCAGGCTAGTGGTACAAATGATCAGAGTCACCTGCTTTCATCATCATTTGCAATCCATGATG
GCTTGACAGCCGTCAGGAGTGGTGGTGTTGGCAGTGGAACTGATGCGATAACCATAGCTTCGTTGAGATATAATGATATG
AATTTGCTACCTGAAAACCCCATCGCAACTTCCAGTTGTTTGGGCGAATCAGGAACCTTCAATTCTCTCGACGATGTGTG
TGGTGTAAACCCATCACAAGGTGGAACCTTTGTGAAGGTTTACAAATCAGGGTCCCCTGGGAGATCGCTCGACATCACCA
GGTTCAGCAGCTACTACGAGTTACGTAGCGAGCTCGAGCGGTTATTTGGTCTTGAAGGCCAACTGGAGGACCCTGTAAGA
TCAGGCTGGCAGCTTGTATTCGTTGACCGGGAAAACGATATTCTTCTCGTCGGCGATGACCCGTGGCAGGAGTTTGTGAA
TAGTGTATGGTGCATAAAGATCCTCTCGCCGCAAGACGTGCAGCAGATGGTCCGCGGCGGAGGCGACCTTCTGTCTACAA
CTGGAGTGAGGACACTGCAGGGCAGTGTCTGCGATGATTATTCTGCAGGCCACGACATGCAGAATCTCACCGGCAGCATC
GCACCTGTGGTGCCTCTGGACTAC
TFome clones do not contain stop codon at 3'end.
Translation: MNLSPPRMSEPSLESDGEQRCLNSELWHACAGPLVSLPVVGSRVVYFPQGHSEQVAASTNKEVDAQIPNYPNLPPQLICQ
LHNVTMHADAETGEVYAQMTLQPLSPEEQKEPFLPIELGAGSNQPTNYFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDF
SQHPPVQELVARDLHDNEWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSIIFIWNDNNQLLLGIRRANRPQTVMPSS
VLSSDSMHIGLLAAAAHAAATNSRFTIFYNPRASPSEFVIPLAKYVKAVYHTRVSVGMRFRMLFETEESSVRRYMGTITC
ISDLDSERWPNSHWRSVKVGWDESTAGDKQPRVSLWEIEPLMAFPMYPTAFPLRLKRPWASGLPMFNGGRSDEFARYSSL
MWLRDGNRGAQSLNFQGLGASPWLQPRIDYPLLGLKLDTYQQMAAAALEEIRAGDHLKQISSLLPVQQPQNLSGGLDPLY
GNPVLQQMQFQSQQSSLQVVQQGYGPNASDSGFLQNQLQLQKQQEPLPQQQQQALQQQSHQEMQHHLSASCHDIANVASG
VSESGSASQTESSLLSGSSYQQIYDGNSGPGLHLHNGFHNCSSQESSNLLNLSRSGQFMASEGWPLKRLAVESLSGHELQ
PVQHKFEKVNHQSNVSHISSTLPPLSARDSYSAQASGTNDQSHLLSSSFAIHDGLTAVRSGGVGSGTDAITIASLRYNDM
NLLPENPIATSSCLGESGTFNSLDDVCGVNPSQGGTFVKVYKSGSPGRSLDITRFSSYYELRSELERLFGLEGQLEDPVR
SGWQLVFVDRENDILLVGDDPWQEFVNSVWCIKILSPQDVQQMVRGGGDLLSTTGVRTLQGSVCDDYSAGHDMQNLTGSI
APVVPLDY
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map