TFome Information for pUT3108

TFome Name:pUT3108
Genbank Insert ID:JX428510
Protein Name:ZmARF23
Species: Maize
TF Family: ARF
Template: BT067427.1
Corresponding TF Gene: GRMZM2G441325
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2939up 3' Primer Name: UT-2939dn
5' Primer Sequence: caccATGGGGATTGATCTCAACGCCGTG 3' Primer Sequence: TATTCCCAAGGGAGCAGCACAGAGAGC
5' Primer Tm (º C): 64.5 3' Primer Tm (º C): 65.3
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 64°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGGGGATTGATCTCAACGCCGTGGGGGAGGACGACCCGGCGGGCGCGGTGTGCGCGGAGCTGTGGCACGCGTGCGCGGG
GGCTGGAGTGGCGCTGCCGCGGCGGGGCAGCGCCGTGGTGTACCTGCCTCAGGCGCACCTCGCGGCGGGCGGCTGCGACG
GTGGTGGGGTGTCGGCGCCGGCCCCGCCGCGCGTGCCGCCGCACGTGGTGTGTCGCGTCGTCGACGTCGAGCTACGCGCG
GATGCGGCCACGGACGAGGTGTACGCGCGGCTTGCGCTGGTGGCGATGGATACGATGTTTGGCCGAAACATCAATGATGG
TGAAACTGAAGAGAAGAATGGCGAGGAAGAGGATGGCGACGGAGAAAAGAAGCACGCATCACACATGTTCTGCAAGACAC
TCACAGCTTCTGATACCAGCACACATGGGGGATTCTCTGTTCCACGGCGAGCTGCAGAGGACTGCTTTCCACCATTGGAC
TATGAGCAGCTTAGGCCTTCCCAAGAGCTTATTGCCAAGGATTTGCATGGCATGAAATGGAGGTTCCGTCATATCTATAG
AGGTCAACCTCGAAGGCATCTTCTGACAACTGGATGGAGTTCATTTATCAATAAGAAGAAACTAGTCTCAGGGGATGCAG
TCTTGTTTCTTAGAGGTAATGATGGTGAGCTAAGATTGGGTGTGAGGAGAGCAGTTCAACTGAAAAATGAAGCTCTACTT
GAAGCTGTCAACTGTACTGATTCGAAGCTACTTATGCTGTCTGCTGTGGCCAATTCTTTGGACAACAGAAGTATATTTCA
CATCTGTTTCAACCCAAGGGTTGGTGCATCAGAATTTATTGTGCCGTATTGCAAGTTCTTGAAGAGCTTGAACTATCACT
TTTCAGTTGGAACCAGATTTAAAGTTGGCTGCGAGAATGAAGATGCTAACGAGAGGTCCTTTGGATTGATCATAGGTATT
AGTGAAGTTGATCCCATACACTGGCCTGGATCAAAATGGAAATCTCTCCTGATAAAGTGGGATGGTGCTACTAAGTACAG
CCACCAGAATAGAGTATCTCCATGGGACATCGAGGGAGTTGGCAGCTCAGTTTCAGTTACTCACCGACTTTCCTCTTCTG
TTTCGAAGCGAACAAAACTGTGCTTCCCTCCAAGCGATTTGGACACGCCAATTCTAGATGGAAACGGTCGTCCAGACTCA
GTGGAAACTGAACGTTTCCACAGGGTCTTGCAAGGTCAAGAATTGGTGCACTCTAGTATTCATGGTACTGCATGCTCTCA
TTCATCAGATAGCCCCAGATGTCAAGGCTCTTATGGCAGGAGATTCTCTGCTGATGCGTGGAACTGCAAGATGAATGATG
TAATGAGTGGGCCTCGACACCTAAATGCCACTGGGTTTGCTTACCAGCCCCTAGGCTTCAGTGAATCTGTCAAATTCTCA
GAGGTCTTGCAAGGTCAAGAAATGTCTCAGGCGGTTCCTTCCTTCATGAGATCTGCTTTCAACTCTGGCACGCAGAATGG
CAGGGTTCGACCATTTGATTATGTGCAGAGATCAGATGCAACTCAAGGATACGCTCTCCAGCAGTTTAATCTGCCAGCAA
CAGAAGTGCATTCGCCCTCTTCTGTTCTTATGTTTAACCAAACCATGGTACCACATGCTGAGCTAGATGGTGCGACCAAC
CGTGAAGAAGTACATGGCAGCAGGTACTTGTCATCCAATGCAATAGGGAGAGAAGCTGAACCATGGCCATCCATGCAGCA
GCAAAGAGCGAGTGTAAATGGAAGCGAGCCTCTCGACACAACTGAAGCCTCAGCTCCTGCAAGGAACGCTGAATCTGGAT
CGGTCGGCAGGGGCGCGGGGCGAAGCAACTGTAAGCTTTTTGGTTTCTCCCTGACTGAGAAGATCCTTGGAACGGATGGT
GGTGGTGTGAAAGAAGGGAACTACGAAGTGGACCGTCAAACTCCCCGGGTGCTAGACTTGTTTGGGCACGGTTCTACGCC
TGGAGCTCTGCATGCTCTCTGTGCTGCTCCCTTGGGAATA
TFome clones do not contain stop codon at 3'end.
Translation: MGIDLNAVGEDDPAGAVCAELWHACAGAGVALPRRGSAVVYLPQAHLAAGGCDGGGVSAPAPPRVPPHVVCRVVDVELRA
DAATDEVYARLALVAMDTMFGRNINDGETEEKNGEEEDGDGEKKHASHMFCKTLTASDTSTHGGFSVPRRAAEDCFPPLD
YEQLRPSQELIAKDLHGMKWRFRHIYRGQPRRHLLTTGWSSFINKKKLVSGDAVLFLRGNDGELRLGVRRAVQLKNEALL
EAVNCTDSKLLMLSAVANSLDNRSIFHICFNPRVGASEFIVPYCKFLKSLNYHFSVGTRFKVGCENEDANERSFGLIIGI
SEVDPIHWPGSKWKSLLIKWDGATKYSHQNRVSPWDIEGVGSSVSVTHRLSSSVSKRTKLCFPPSDLDTPILDGNGRPDS
VETERFHRVLQGQELVHSSIHGTACSHSSDSPRCQGSYGRRFSADAWNCKMNDVMSGPRHLNATGFAYQPLGFSESVKFS
EVLQGQEMSQAVPSFMRSAFNSGTQNGRVRPFDYVQRSDATQGYALQQFNLPATEVHSPSSVLMFNQTMVPHAELDGATN
REEVHGSRYLSSNAIGREAEPWPSMQQQRASVNGSEPLDTTEASAPARNAESGSVGRGAGRSNCKLFGFSLTEKILGTDG
GGVKEGNYEVDRQTPRVLDLFGHGSTPGALHALCAAPLGI
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map