TFome Information for pUT3110

TFome Name:pUT3110
Genbank Insert ID:JX428512
Protein Name:ZmARF14
Species: Maize
TF Family: ARF
Template: BT087991.1
Corresponding TF Gene: GRMZM2G137413
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2907up 3' Primer Name: UT-2907dn
5' Primer Sequence: caccATGGCGAGCGCCGGCGTG 3' Primer Sequence: ATCAGATTCAACGTCTACTGAGAGAGCAGGTG
5' Primer Tm (º C): 66.8 3' Primer Tm (º C): 63.9
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 64°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGGCGAGCGCCGGCGTGGCGGGCTGCTCAGGGAGTGCTGGCGATGCTTTGTTCAGGGAACTCTGGCATGCTTGTGCTGG
GCCGTTGGTCACAGTGCCTCGCCAAGGCGAGCTGGTTTACTACTTCCCACAGGGTCATATGGAACAGCTTGAAGCATCTA
CAGATCAACAACTTGATCAGCACTTACCTTTGTTTGATCTACCACCCAAGATTCTCTGCAAGGTGGTCAATGTAGAACTT
AGAGCTGAAACTGATTCTGATGAAGTTTATGCTCAAATTATGCTGCAACCAGAAGCAGATCAAAGTGAGCCCACCAGCCC
AGATTCTGAGCCACCGGAGCCTGAAAGATGCAACGTCTATTCCTTCTGCAAGACCTTGACTGCTTCAGATACAAGTACCC
ATGGTGGATTCTCTGTCCTCAGGAGGCATGCTGAAGAATGTTTGCCCCAACTGGACATGACTCAGAATCCACCATGGCAA
GAACTGCTGGCTAAAGATCTCCATGGAAATGAATGGCATTTCCGTCACATCTTTCGAGGGCAACCAAGGAGGCATCTACT
TACGACAGGTTGGAGTGTTTTTGTTAGCTCAAAAAGATTGGTTGCTGGTGACGCGTTTATCTTTTTGAGAGGTGAGAACG
GAGAGCTGAGAGTTGGGGTAAGGAGGCTCATGAGGCAACTAAATAACATGCCATCTTCGGTTATCTCAAGCCACAACATG
CATCTTGGAGTCCTAGCAACTGCATCTCATGCCATCTCCACAGGAACTCTCTTTTCTGTTTTCTACAAACCTAGAACAAG
TCGATCGGAATTTGTCGTTAGTGTAAATAAGTACCTTGAAGCTAAGAATCACAAGGTGTCTGTTGGCATGAGGTTTAAAA
TGAGATTTGAGGGTGATGAATCTCCTGAAAGAAGATTAAGTGGGACAATTATTGGTCTGGGAAGCATGCCAGCTAACTCA
ACTTCTCCATGGGCTAACTCTGATTGGAGATCTTTGAGGGTCCAATGGGACGAGCCTTCTGCTATTCTGCGTCCAGATAG
AGTTTCACCATGGGAACTAGAACCCCTTGATGCAACTAATCCACAACCACCTCAACCTCATTTACGGAATAAGCGTGCAC
GACCCCCTGCTTTACTTTCTATTGCTCCAGAACTTCCTCAAGTTTTTGGTTTTTTGAAATCCCCAGCTGAGCCTGCCCAA
GCTTTCTCATTTTCAAGACCGCAGCAAACTCAGGAATTATACCATTCAAATCCCAGTTCAATCTTCTCATCATCGTTGAA
TGTAGGATTCAGTTCAAAGAATGACCGTTCTACTCCAATCAACAGTCATTTGTACTGGACAATGAGGCAGACAAGAACTG
AATCCTACTCTGCTAGCATTAACAAAGCTCCTACTGAAAAGAAGCAAGAATCTGCTACTTCTGGCTGCAGATTGTTTGGC
ATTGAGATAGGTTCTGCAGTATCACCAGTGGCTACTGTTGCTAGTGTTGGCCAGGATCAGCCACCTGCTCTCTCAGTAGA
CGTTGAATCTGAT
TFome clones do not contain stop codon at 3'end.
Translation: MASAGVAGCSGSAGDALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCKVVNVEL
RAETDSDEVYAQIMLQPEADQSEPTSPDSEPPEPERCNVYSFCKTLTASDTSTHGGFSVLRRHAEECLPQLDMTQNPPWQ
ELLAKDLHGNEWHFRHIFRGQPRRHLLTTGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRLMRQLNNMPSSVISSHNM
HLGVLATASHAISTGTLFSVFYKPRTSRSEFVVSVNKYLEAKNHKVSVGMRFKMRFEGDESPERRLSGTIIGLGSMPANS
TSPWANSDWRSLRVQWDEPSAILRPDRVSPWELEPLDATNPQPPQPHLRNKRARPPALLSIAPELPQVFGFLKSPAEPAQ
AFSFSRPQQTQELYHSNPSSIFSSSLNVGFSSKNDRSTPINSHLYWTMRQTRTESYSASINKAPTEKKQESATSGCRLFG
IEIGSAVSPVATVASVGQDQPPALSVDVESD
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map