TFome Information for pUT3111

TFome Name:pUT3111
Genbank Insert ID:JX428513
Protein Name:ZmCA5P10
Species: Maize
TF Family: CCAAT-HAP5
Template: BT055482
Corresponding TF Gene: GRMZM2G440949
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2906up 3' Primer Name: UT-2906dn
5' Primer Sequence: caccATGAGGAAGAAGCTGGACACG 3' Primer Sequence: GTCTTCATTGTCATAATCCTCGTC
5' Primer Tm (º C): 53.3 3' Primer Tm (º C): 51.4
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 52°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGAGGAAGAAGCTGGACACGCGTTTCCCCGCTCCTCGGATTAAAAAGATCATGCAAACAGATGAAGACGTTGGCAAGAT
TGCTCAAGCTGTACCTGTTCTAGTGTCCAAAGCATTGGAGCTATTTCTGCAAGATTTATGTGATAGGACATATGATATTA
CAATTCGGAAGGGGGTCAAGACTGTGGGTTCTTCCCATTTGAAACAATGCATACAAACGTACAATGTCTATGACTTCTTG
AGGGAAGTAGTCAGCAAAGTTCCAGACATTGGTCCTTCTGATGTTATTGCTGATGATAAGCTTGGCAAAAGGAGGAAAGC
CGAAGAAGATGGAAGTGAGGAAGAATTGAAGAGGACAAGAAATGAGACAGAAAGCCATACAAGCAATGGCAGAGGCCGTG
GGAGAGGTCGTGGGAGAGGTCGCCGTAGTGTACGTGGACCTTGGCGGGAGGTGGTCACAAGTCCTGAACAATTCAAGGAG
GAGAATCAATCTAGCAAGGTAGCCAGCCTAAAGGTGGAGGTAGCAGATGAGGTCTCGAATGTAACTGAAGCAAAACAGGA
CACTACACCAATGAGCAATGCCAGGCCTTCCTTAAGGAACATTGACTTGAATTTAGATCCAACCGATGAAGAGGATGAGG
TTACAGTGCCACCCGAACCCCAGCTGTCAGCTCCAGCAACTAGTTGCGCTGCAGCCACTGCAGCACCTGGACCATCTGTT
CCACGGTTAAGGGAAGGAGCAAAACTCAAGGACTTCTTTGGTGCTTGGGAACTGGCTGACATGAACAAGATGGAGATGGA
CCCTGTCCAGTTTGCTCTGTCAACAAACCATAGATTGGAGGAGGACGAGGATTATGACAATGAAGAC
TFome clones do not contain stop codon at 3'end.
Translation: MRKKLDTRFPAPRIKKIMQTDEDVGKIAQAVPVLVSKALELFLQDLCDRTYDITIRKGVKTVGSSHLKQCIQTYNVYDFL
REVVSKVPDIGPSDVIADDKLGKRRKAEEDGSEEELKRTRNETESHTSNGRGRGRGRGRGRRSVRGPWREVVTSPEQFKE
ENQSSKVASLKVEVADEVSNVTEAKQDTTPMSNARPSLRNIDLNLDPTDEEDEVTVPPEPQLSAPATSCAAATAAPGPSV
PRLREGAKLKDFFGAWELADMNKMEMDPVQFALSTNHRLEEDEDYDNED
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map