TFome Information for pUT3116

TFome Name:pUT3116
Genbank Insert ID:JX428517
Protein Name:ZmARF5
Species: Maize
TF Family: ARF
Template: BT067327.1
Corresponding TF Gene: GRMZM5G808366
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2928up 3' Primer Name: UT-2928dn
5' Primer Sequence: caccATGGGGCCACAGCCACCGCT 3' Primer Sequence: GACATCTTTTCCGACACTGCCATCAGCC
5' Primer Tm (º C): 65.2 3' Primer Tm (º C): 67
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 65°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGGGGCCACAGCCACCGCTTCACCTTCCCGCCGCCAACGGCGACAGCATCGTCGACCGCGACGTTTGGCTCGCCTGCGC
CGTCCCGCTCTCCCGCCTCCCCGCCGTCGGTGCCGAGGTCTACTACTTCCCGCACGGCCACGCCGAGCAGTGCCCCGCGC
ATCTCCCGGCGCCGCTCCCGGCGCCGCACCTCTTCCCTTGCACCGTCGCCGGCGTCAGCCTTGGCGCGGACGACGAGACC
AACGAGGTGTTCGCCAAAATCTCCCTCAGCCCAGGCCCCCACCGCGGGCCCGCCGCCGCTTGTCGTACCGACCCCACCAG
CGACTGCCCGCCGCAGGAGCTCAGTTACTTCACCAAGGAGCTCACGCAGAGCGACGCCAACAACGGCGGCGGATTCTCCG
TCCCGCGCTACTGCGCCGACCACATCTTCCCCACGCTCGACTTCGACGCCAACCCGCCCGTGCAGAAGCTTTTCATGCGC
GACACCAGGGGCAACCCGTGGCAGTTCCGCCACATCTACCGCGGCACGCCACGCCGCCACCTGCTTACCACCGGCTGGAG
CAGGTTCGTCAACGCCAAGCTGCTCGTCGCGGGGGACATCGTCGTCTTCATGCGCCGCCACAACGGCGACCTCATCGTCG
GCCTGCGCCGCACGCCCAGGTACCCGCTCGTCTTCCCCAGGGTCGGGTCCGGAGCCGGGGTCGACCCGGACCAGCCCCCG
CCCCGCAACGCGCGCGCGCGGGTACCGCCCCAGGACGTCATTGAGGCCGCACGCCTCGCCGCGGAGGGCAGGTCCTTCGC
GGTCACCTACTTCCCGAGACAGGCCGCCGGGGAGTTCATCGTCCCGCGCGACGAGGTGGAGGGCGTGCTCGCCACCCGCT
GGGAGCCAGGCGCGCAGGTGCGTATGCAGGTCATGGAGGCCGAGGACACGCGGCGCACCGTGTGGGCCGACGGCCACGTC
AAGTCGCTCCACCAAAACATCTGGCGAGCGCTCGAGATCGACTGGGATGATTCCTCTCCATTATCACCAAATCTGAGCAG
ATTTGTGAATGCTTGGCAAGTCGAGCTTGTTACACACCCTCCTCTTCCAAATGGAGCGAGGATCTGCAACCCAATTGCGT
CTCTTTGCCATGGAGATGTCTCTTATCCACTGATCGGATCGGAAATCCAGGGGCCACCAATTCCTGCTAGCATGCAGGGA
GCCAGGCACACCGGCCCTTGTGCTGCGCCCTCTGAATCTTCTGCAGTGCTCACGAATCGGGTACTGTTCCCCCTGCTCAA
CCCGGACCTCCAGATGCCACCGTTTACAAGCCCCAGTGATTCCTCTGAGATTCTGGATCCTGAATCTGCTTCTCCTCCCA
ACAAATCAGTAAGGTTGCCTCCAGCAGAGCTTCCAGTCCAAGTGAAGAGCATACAGCTCTTTGGCGCCACAATCATGGTC
CATGTTGTGCGAAGTGTTGCCTGTGGTGGTTGTTGTGAAGAACTGAATGGAGCTCCTAGTGGTGATGCTACGGCTGATGG
CAGTGTCGGAAAAGATGTC
TFome clones do not contain stop codon at 3'end.
Translation: MGPQPPLHLPAANGDSIVDRDVWLACAVPLSRLPAVGAEVYYFPHGHAEQCPAHLPAPLPAPHLFPCTVAGVSLGADDET
NEVFAKISLSPGPHRGPAAACRTDPTSDCPPQELSYFTKELTQSDANNGGGFSVPRYCADHIFPTLDFDANPPVQKLFMR
DTRGNPWQFRHIYRGTPRRHLLTTGWSRFVNAKLLVAGDIVVFMRRHNGDLIVGLRRTPRYPLVFPRVGSGAGVDPDQPP
PRNARARVPPQDVIEAARLAAEGRSFAVTYFPRQAAGEFIVPRDEVEGVLATRWEPGAQVRMQVMEAEDTRRTVWADGHV
KSLHQNIWRALEIDWDDSSPLSPNLSRFVNAWQVELVTHPPLPNGARICNPIASLCHGDVSYPLIGSEIQGPPIPASMQG
ARHTGPCAAPSESSAVLTNRVLFPLLNPDLQMPPFTSPSDSSEILDPESASPPNKSVRLPPAELPVQVKSIQLFGATIMV
HVVRSVACGGCCEELNGAPSGDATADGSVGKDV
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map