TFome Information for pUT3121

TFome Name:pUT3121
Genbank Insert ID:JX428522
Protein Name:ZmABI47
Species: Maize
TF Family: ABI3-VP1
Template: BT038952
Corresponding TF Gene: GRMZM2G125596
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2909up 3' Primer Name: UT-2909dn
5' Primer Sequence: caccATGGGCCAGATGGGAGGACC 3' Primer Sequence: GGGGTTTGTCTGCTGAATGGACTT
5' Primer Tm (º C): 59.4 3' Primer Tm (º C): 59.3
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGGCCAGATGGGAGGACCCGACGGCGACGGCGACGGCGGCGCGGGCCCGCACCACCAGTACCACTACCAGGCGCTTCT
CGCCGCCGTGCAGAACCCCAGCCAGGGCCTGCACCCCTTTCCCCTCCCCTTCCACGTGCCTCTGCACGCAGGAGCGGGAG
CACCCGCCGCCGGACCAGGACCTGGAGCTGATGCTGATGCCTCCACACACAATGCCAATGCCGCTCACCACTCTCAACCT
CCAAGAGGTTTCACCGATTGGAGCGCGTCCAACAGTGCCTTCGCCGCCGTTGCCTCACAGCCCGCACCTGCTACCACCAA
TACACCCTTCCATTACAACCTCTCCCAGTCTTATGCCCTATGGACCCATTACATGCTTAACAAGAATGTATCCTACTCTA
CTTACCCTACACCACATGAGGAGCATCCCCACCCATTGCGCCATACCCACATCCAGGAGAATCCCCACCCATTGCGCCAT
ACCCACATCCCGGATAAGGATTCAGGTTGTGCATCTAGCCTTGGATTTGACTCTTTCACTACAATGTCCCTTGGACCAAA
TATTTGTTCCCACATGACGCCCATGGAAGGATCTATATCTGCCAAAGAACCCGAGAATTCAGAGGATTTGCCTGCAATAG
TTAGGAGCAGTGATGAAATGGACACTAGAAACAGTGGCAAAGTTCATCGTGACACAGTTGGCACTCTTCCTGAGTCGAAG
CAGAGCCATGAAAGTTGTGCTTCCGTGAATAATAAGTTCAACTCTGGAGAGTACCAAGTCATTTTGCGCAAGGAGTTGAC
AAAGAGTGATGTCGCAAATTCCGGACGAATTGTGCTTCCCAAGAAGGATGCTGAGGCTGGTCTTCCACCATTGGTGCAAG
GGGATCCTCTGATACTGCAGATGGATGACATGGTGCTTCCAATTATATGGAAATTTAAGTATAGATTTTGGCCAAACAAC
AAAAGCAGAATGTATATCTTGGAAGCTGCAGGTGAATTCGTGAAGACACATGGCCTTCAGGCAGGGGATGCGCTCATTAT
CTACAAAAACTCCGTGCCTGGCAAATTTATTATCCGTGGGGAGAAGTCCATTCAGCAGACAAACCCC
TFome clones do not contain stop codon at 3'end.
Translation: MGQMGGPDGDGDGGAGPHHQYHYQALLAAVQNPSQGLHPFPLPFHVPLHAGAGAPAAGPGPGADADASTHNANAAHHSQP
PRGFTDWSASNSAFAAVASQPAPATTNTPFHYNLSQSYALWTHYMLNKNVSYSTYPTPHEEHPHPLRHTHIQENPHPLRH
THIPDKDSGCASSLGFDSFTTMSLGPNICSHMTPMEGSISAKEPENSEDLPAIVRSSDEMDTRNSGKVHRDTVGTLPESK
QSHESCASVNNKFNSGEYQVILRKELTKSDVANSGRIVLPKKDAEAGLPPLVQGDPLILQMDDMVLPIIWKFKYRFWPNN
KSRMYILEAAGEFVKTHGLQAGDALIIYKNSVPGKFIIRGEKSIQQTNP
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map