TFome Information for pUT3124

TFome Name:pUT3124
Genbank Insert ID:KJ726806
Protein Name:ZmARF28
Species: Maize
TF Family: ARF
Template: BT066544.1
Corresponding TF Gene: GRMZM2G006042
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGTGGCGGCTGCAGCGGCGGCCGGCGGCGGCGCCGACGCGGGATGCGGAGGCGGAGGCGGAGGCGGAGGGAAGGACGC
GCTGTTCGTGGAGCTGTGGAAGGCCTGCGCGGGGCCGCTGTCCAGCGTGCCGCTACTCGGGGAGAAGGTCTACTACTTCC
CGCAGGGCCACATCGAGCAGGTGGAGGCATCGACAAACCATCTTGCCGAGCATCAGGGCACGCCCCTCTACAACCTGCCA
TGGAAGATACCCTGCAAGCTCATGAACATGGAGCTGAAGGCTGAGCCGGATACTGATGAGGTGTACGCCCAACTCACCCT
GCTCCCTGATAAGAAGCAAGATGAGAATACATCTGCAAGAGTGGAGAATGAGGAGGCAGAGGAGGAGGTGGTGCCTCATG
TGCCACCAGCCACAAGTGAGGGGCTTCGCATCCATTCCTTCTGCAAGACATTGACTGCCTCAGACACAAGCACACATGGT
GGTTTCTCAGTGTTGCGCCGGCATGCAGATGAGTGCCTCCCACCACTGGATATGAGTCAACATCCCCCAAATCAAGAATT
GGTGGCCAAGGACCTGCATGGGGTTGAGTGGCGGTTCCGTCACATCTTTCGAGGTCAGCCACGGAGACACCTCCTTCAGA
GTGGTTGGAGTGTTTTTGTTAGTGCCAAGCGTCTTGTTGCGGGGGATGCATTCATATTTCTCAGAGGTGAGAATGGGGAG
TTACGTGTTGGAGTTCGGCGTGCACTGAGGCACCAAACCACTATCCCATCTTCAGTCATATCAAGCCACAGTATGCATCT
TGGTGTTCTTGCAACAGCATGGCATGCAGTGAACACTGGGAGTATGTTCACTGTCTACTACAAACCAAGGACTAGTCCAG
CTGAATTTGTGGTTTCACGAGCTAGGTACTGTGAATCACTGAAACGAAATTATTCAATAGGGATGAGGTTTAGGATGAGA
TTTGAAGGTGAAGAAGCGGCAGAGCAAAGATTCACTGGCACAATAGTTGGAATATGTGTTTCTGATCCATCTGGCTGGGC
TGACTCGAAATGGCGTTCCCTTAAGGTGAGATGGGATGAAGCTTCTTCTGTTCCTCGTCCTGAAAGAGTTTCTCCTTGGC
AAATAGAACCTGCTGTTAGCCCATCTCCTGTGAACCCTCTTCCAGTCAGATTCAAGAGGTCTCGTTCAAGTGTTAACGCC
TCACCATCTGATGTGTCTACTGTAAACAGAGAAGTTGCTTCTAAAGTCATGGTAGAGTCGCAACAGAATAATCTCCCAAG
GGCCTTGCACAATCAAGGAAGGACACAATTGACAGGTCGATACCGTGATAGCACTGATGTGAAGACTGCTCAGGATCTTA
CCATGTGGTCTTCAGGAACTGAGCAACAGAGAAACAATATTGCTGCCCAGACAAAACGATGTCTGGAGGGTTGGACGCAA
TCTAGAACTCCAGAAGGTTACAATCAACTGTTCTCGGCATTTCAACCACTGAAGGATGCACATAATCCACTTCGTCCTTT
TCCTAATAAAATATCTGGGACTCGCTCAAGTACCTGGGTCACAGCCGATGCCCGTTATCCAGCTCAACAAGCCAATCACA
ACATGTTACATGGCACATTGTCTTTTATGCCTCATAGCAGTGGTTTTAGGATGATTCAACAGAATAATCTAGTGACACCT
GAAGCTGCAAATTTTACTGGGAAATCAGCTTTCACTTCACTTCAGGGCCATGTCACTGATCAGTGCTCCACAGGCTGGTT
TGGATCTATTGAGTCAAATTCCCACACAGACCATGCATCGTCAAGTTTGATCAGGTCCCAGCCTTTGGTTATTGGAAATG
ATGTTCAGAAAACCAAAGGCACTTCATTCAAACTATTTGGGATTCCTCTTGGCAGTCCAGAAAAATCTGAACCTCTGGTA
TCCCCACCAAGTGTCGCATATGACGGGAAACTTCAAACTTCTCCAACAGACAACAATGAGCCTTGTTCAGAAGCTACCCA
GAATATCCAGAACAAAGTGCAAAGTAGTTCAACCAGAAGCTGCAAGAAGGTCCATAAGCAAGGCAGCGCCCTCGGGAGAT
CGATCGATCTAACAAAGTTCGCTTGCTACGATGAGCTGATTGCCGAACTAGACCAGATGTTTGACTTCGATGGTGAGCTG
AAGAGTCCTTGCAGGAACTGGTTGGTCGTCTACACTGACAACGAAGGCGACATGATGCTGGTCGGCGACGACCCCTGGAA
TGAGTTCTGTGACATGGTCCACAAGATCTTCATCTACACAAGGGAGGAAGTGGAGAGGATGAACCCAGGCGCCCTAAACG
CGAGGCTCGAAGACTGCCTGTCTGACTCGCTGGGAAGAGGGCTGGCTTCCAAAGAGCCCCGGAGCGGCCCCTCGACTTCG
GCTGTCGATTCTGAGAACCGCGCTAACTTGTCCAGTCAG
TFome clones do not contain stop codon at 3'end.
Translation: MVAAAAAAGGGADAGCGGGGGGGGKDALFVELWKACAGPLSSVPLLGEKVYYFPQGHIEQVEASTNHLAEHQGTPLYNLP
WKIPCKLMNMELKAEPDTDEVYAQLTLLPDKKQDENTSARVENEEAEEEVVPHVPPATSEGLRIHSFCKTLTASDTSTHG
GFSVLRRHADECLPPLDMSQHPPNQELVAKDLHGVEWRFRHIFRGQPRRHLLQSGWSVFVSAKRLVAGDAFIFLRGENGE
LRVGVRRALRHQTTIPSSVISSHSMHLGVLATAWHAVNTGSMFTVYYKPRTSPAEFVVSRARYCESLKRNYSIGMRFRMR
FEGEEAAEQRFTGTIVGICVSDPSGWADSKWRSLKVRWDEASSVPRPERVSPWQIEPAVSPSPVNPLPVRFKRSRSSVNA
SPSDVSTVNREVASKVMVESQQNNLPRALHNQGRTQLTGRYRDSTDVKTAQDLTMWSSGTEQQRNNIAAQTKRCLEGWTQ
SRTPEGYNQLFSAFQPLKDAHNPLRPFPNKISGTRSSTWVTADARYPAQQANHNMLHGTLSFMPHSSGFRMIQQNNLVTP
EAANFTGKSAFTSLQGHVTDQCSTGWFGSIESNSHTDHASSSLIRSQPLVIGNDVQKTKGTSFKLFGIPLGSPEKSEPLV
SPPSVAYDGKLQTSPTDNNEPCSEATQNIQNKVQSSSTRSCKKVHKQGSALGRSIDLTKFACYDELIAELDQMFDFDGEL
KSPCRNWLVVYTDNEGDMMLVGDDPWNEFCDMVHKIFIYTREEVERMNPGALNARLEDCLSDSLGRGLASKEPRSGPSTS
AVDSENRANLSSQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map