TFome Information for pUT3137

TFome Name:pUT3137
Genbank Insert ID:JX428537
Protein Name:ZmABI50
Species: Maize
TF Family: ABI3-VP1
Template: BT035634
Corresponding TF Gene: GRMZM2G102938
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2776up 3' Primer Name: UT-2776dn
5' Primer Sequence: caccATGGAGGTCCGTGGCAGTGC 3' Primer Sequence: GTCCACAATGACGAGTGGATCGTC
5' Primer Tm (º C): 66 3' Primer Tm (º C): 59.4
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGAGGTCCGTGGCAGTGCACCCAGCATGAAGCCAAAGGTGGAGCCGCCGTTGGAGGCTTCACACGACTGGGAGGTGAC
CCCGCTGTCCGGCGACAACCCCTTCTTCACCAGCGTCATGCGCAAGAGCCAAGTCCAGAACCCCTTTCAGCTGGTACACA
TTTTCTTTTCTTTTCTTTTTCCCCAATCCGGAACGCACGCACGTAGGAGCACACCGTACTGTTTGTTCCAGGTCATCCCG
GTTCGCTTCCACCGCCACCTCCCGGAGGCGCGCGCGCCCGCGGTGCTCCTCTGCCGCGACCGGTCGTGGACGGTGAGCTA
CGCGGGCGTCGGCAAGTGGAAGAGGCTCCAGGGGGCGTGGAGGGACTTCGCCCGCGACAACGGGCTCCGCCTCGGGGACG
CCTGCGTCTTCGAGCTCCTTGTGCCCAGTACTGATGCAACCCAAGGAGACGGAGACGGCAGCAAGGATCAGGGGGAGGTG
GTGTTCCGTGTGCAGGTGCTCCGCGGCGGCCTGCCGGCTGAGATCACCTCCCGGGGCGCCACCTCCGACGATCCACTCGT
CATTGTGGAC
TFome clones do not contain stop codon at 3'end.
Translation: MEVRGSAPSMKPKVEPPLEASHDWEVTPLSGDNPFFTSVMRKSQVQNPFQLVHIFFSFLFPQSGTHARRSTPYCLFQVIP
VRFHRHLPEARAPAVLLCRDRSWTVSYAGVGKWKRLQGAWRDFARDNGLRLGDACVFELLVPSTDATQGDGDGSKDQGEV
VFRVQVLRGGLPAEITSRGATSDDPLVIVD
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map