TFome Information for pUT3143

TFome Name:pUT3143
Genbank Insert ID:JX469903
Protein Name:ZmARID4
Species: Maize
TF Family: ARID
Template: BT055100.1
Corresponding TF Gene: GRMZM2G138976
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2894up 3' Primer Name: UT-2894dn
5' Primer Sequence: caccATGATGGAAGGGCCTTCCTCATG 3' Primer Sequence: AGCATTTCGTGGTACAGATGCTGTATTT
5' Primer Tm (º C): 58.6 3' Primer Tm (º C): 59.1
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 58°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGATGGAAGGGCCTTCCTCATGGTCAGCCGTCAAGGCTAAATTAAAGCCTGCAACTATGAGGCCCATTCCTCACTCTCG
AAAGCAACAAATGCATCCATTTATGGGTTTTCCTGAAACTTCCCTTCACGAAACTAGTATTGTGAAGCCAAGCTTGCCAG
CTGCACCAACTGTGAAGCACAATTCAGTTTCTTCTGCCACAACCACGCACAGAAAATCAACATCAGGGCCGTCTCATACT
CCCTCAGTCATTCAGTTAAATCCTCTGCCAATGAAGAAACACGGATGTGATAGGTTGCCTATCCAAGTGTGCTCTGAGGA
GGATTTTTTGAAGGATGTCATGCAGTTTTTAATCCAGAGGGGCCACAATCGACTTGTACCTCACGGAGGACTAGCTGAAT
TTCCTGATGCAATCCTTAACGCCAAGCGCCTTGATCTTTATAACCTGTACAAAGAGGTGGTGTCTAGAGGTGGATTTTAT
GTGGGTAATGGCATAAACTGGAAGGGTCAAGTTTTTTCAAAGATGCGTAACCATACCGCAACAAACAGAATGACTGGTGT
AGGTAACACGTTGAAAAGACATTACGAGACATACTTGTTGGAATATGAGTTGGCACATGATGACGTGGATGGCGAATGCT
GCTTACTTTGTCACAGTAGCGCACCTGGAGATTGGGTGAACTGCGGTTTATGTGGTGAATGGGCTCACTTTGGCTGCGAC
AGACGACAAGGACTAGGCACTTTCAAGGATTACGCTAAGACAGATGGTTTGGAATACATCTGCCCCCACTGTAGCTTAGC
AAACTACAAGAAGAAGCCACCACCACCAAAAGCAGCTAACGGTTTTTCAAATACAGCATCTGTACCACGAAATGCT
TFome clones do not contain stop codon at 3'end.
Translation: MMEGPSSWSAVKAKLKPATMRPIPHSRKQQMHPFMGFPETSLHETSIVKPSLPAAPTVKHNSVSSATTTHRKSTSGPSHT
PSVIQLNPLPMKKHGCDRLPIQVCSEEDFLKDVMQFLIQRGHNRLVPHGGLAEFPDAILNAKRLDLYNLYKEVVSRGGFY
VGNGINWKGQVFSKMRNHTATNRMTGVGNTLKRHYETYLLEYELAHDDVDGECCLLCHSSAPGDWVNCGLCGEWAHFGCD
RRQGLGTFKDYAKTDGLEYICPHCSLANYKKKPPPPKAANGFSNTASVPRNA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map