TFome Information for pUT3144

TFome Name:pUT3144
Genbank Insert ID:JX469904
Protein Name:ZmARID9
Species: Maize
TF Family: ARID
Template: BT033548.1
Corresponding TF Gene: GRMZM5G866423
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2899up 3' Primer Name: UT-2899dn
5' Primer Sequence: caccATGTCATTTGGTGCAAAAGGAGTTGTAG 3' Primer Sequence: TGGTGCAGGCAACGGCG
5' Primer Tm (º C): 62.4 3' Primer Tm (º C): 57.6
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 58°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), 1XGC buffer (NEB), 3.5 mM MgCl2, 10% Glycerol, 40pg/ul template.
Nucleotide Sequence: ATGTCATTTGGTGCAAAAGGAGTTGTAGGGGATAATACGATCTCCCTATCATCATTGAAACCAAGGCTAAAGCCTGCAAC
AATGAGACCCCTTCCTCACTCTCGCAAGCAACAAATGCATCCCTTTATGGGGTTTCCTCAGTCCATCATTCACGATGCTA
GTCAAGTTAAGCCTAGCTTGCCAGCCCCTCCTGTGAAGCACAACGCAGTACCTGTTACCGCAGTGACACCGAGAAAATCA
TCATCTGGGTCACCATCATCCTGGTCACGTGTTCAGCTACCAATCCCATTGAATCCTCTGCCAATAAAGAAACATGAGTG
CAATCGGTTGCCTATCCACAGTTGCTCTGAAGAAGACTTCTTGAAAGATGTCATGCAGTTTCTACTTCAGAGGGGCCACA
TCCGACTTGTACCTCAGGGAGGACTGGCAGAATTTCCTGACGCAGTGTTAAATTCAAAGCGTCTTGATTTATATAACTTA
TACAAAGAGGTGGTATACCGAGGTGGCTTTTATGTAGGCAATGGCATAAATTGGAAAGGTCAAATCTTTTCAAAGATGCA
CAACCATACAGTTACAAACAAAATGACGGGTGTTGGGAACACCCTAAAAAGGCATTATGAGACGTACTTGCTTGAATATG
AACTTGCACATGACGATATTGATGGGGAATGTTGCTTGATTTGCCACAGTGGTGCTCTGGGTGACTGGGTGAACTGTGGA
TTATGTGGAGAATGGGCCCACCTTGGCTGCGATAGACGGAAAGGGCTGTCTACTTTCAAGGACTACTCGAAGGCGGATGG
GATGGAATACATATGTCCCCAGTGTAGCCTTGCGAAATACAAACCGCCGTTGCCTGCACCA
TFome clones do not contain stop codon at 3'end.
Translation: MSFGAKGVVGDNTISLSSLKPRLKPATMRPLPHSRKQQMHPFMGFPQSIIHDASQVKPSLPAPPVKHNAVPVTAVTPRKS
SSGSPSSWSRVQLPIPLNPLPIKKHECNRLPIHSCSEEDFLKDVMQFLLQRGHIRLVPQGGLAEFPDAVLNSKRLDLYNL
YKEVVYRGGFYVGNGINWKGQIFSKMHNHTVTNKMTGVGNTLKRHYETYLLEYELAHDDIDGECCLICHSGALGDWVNCG
LCGEWAHLGCDRRKGLSTFKDYSKADGMEYICPQCSLAKYKPPLPAP
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map