TFome Information for pUT3179

TFome Name:pUT3179
Genbank Insert ID:JX469923
Protein Name:ZmbZIP30
Species: Maize
TF Family: bZIP
Template: BT034699.2
Corresponding TF Gene: GRMZM2G095078
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.C768T p.(=). In template FlcDNA.

5' Primer Name: B443_F 3' Primer Name: B443_R
5' Primer Sequence: caccATGGCGTCGTCCTCCGA 3' Primer Sequence: CCTAGTAGTGTTAGCCTCCGGTTTGTG
5' Primer Tm (º C): 69.2 3' Primer Tm (º C): 66.8
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCGTCGTCCTCCGACGAGCAGTCCAAGCCGCCGGAGCCGCCCGCCGCCGCCGCCGTGGTCACCGCCGCAGCACCGCC
ACAGACGCACGCCGAGTGGGTCGCTTCGCTTCAGGCCTACTACGCTGCCGCGGGGCACCCCTACGCCTGGCCGGCGCAGC
ACCTCATGGCGGCGGCTGCGGCGGGGGCGCACTTCGGCACGCCGGTGCCGTTCCCCGTCTACCACCCAGGCGCCGCCGCG
GCGTACTACGCGCACGCATCCATGGCCGCGGGCGTCCCTTACCCGACGTGCGAAGCTGTCCCCGCGGTGGCGCTGCCCAC
TGTGCCGGAAGGGAAAGGGAAGGGTAAGGGCGGAGGCGCGTCGCCTGAGAAAGGCAGCTCCGGGGCGCCCTCCGGCGAGG
ACGCTTCTAGGAGCGATGACAGCGGCAGCGATGAGTCATCGGAGACTAGAGATGATGACACTGACCATAAGGATTCATCT
GCGCCCAAGAAGAGGAAATCTGGTAACACATCGGCTGAAGGTGAGCCGTCTCAAGCTACTGTTGTGCGATATGCTGCGGT
TGAGTCACCATATCCCGCAAAAGGAAGGTCTGCCTCAAAGCTTCCAGTGTCTGCACCTGGGCGTGCAGCGCTTCCTAGTG
CCACCCCGAATCTAAACATTGGGATGGACATTTGGAATGCTTCTCCTGCCTTGGCTGTGCCTGCAGTGCAGGGGGAAGTG
AGTCCTGGGTTGGCACTTGCCCGACGTGATGGCGTTACTCAACTGGATGAACGTGAAATAAAGAGGGAGAGGCGAAAACA
ATCTAACAGGGAGTCTGCAAGGAGATCTAGATTACGCAAGCAGCAAGAGTGCGAGGAGTTAGCCCGGAAGGTAGCTGACC
TAACGACCGAGAACAGCGCTCTCAGAGCAGAACTTGACAACCTCAAGAAGGCTTGTCAAGACATGGAAGCAGAAAATTCA
CGTCTGTTGGGTGGGGTGGCTGACGCCCAGGTACCAAGTGTCACGACCACACTGGGAATGAGCATCGAGCCGCCGAAGTT
GCAGCTGCAGCTGCAGCAGCATCATGATGAGGAGGGCCAGCTCCACAAGAAATCTAGTAATAACAGCAATGGGAACTGTG
CTGGAGGCAGCCACAAACCGGAGGCTAACACTACTAGG
TFome clones do not contain stop codon at 3'end.
Translation: MASSSDEQSKPPEPPAAAAVVTAAAPPQTHAEWVASLQAYYAAAGHPYAWPAQHLMAAAAAGAHFGTPVPFPVYHPGAAA
AYYAHASMAAGVPYPTCEAVPAVALPTVPEGKGKGKGGGASPEKGSSGAPSGEDASRSDDSGSDESSETRDDDTDHKDSS
APKKRKSGNTSAEGEPSQATVVRYAAVESPYPAKGRSASKLPVSAPGRAALPSATPNLNIGMDIWNASPALAVPAVQGEV
SPGLALARRDGVTQLDEREIKRERRKQSNRESARRSRLRKQQECEELARKVADLTTENSALRAELDNLKKACQDMEAENS
RLLGGVADAQVPSVTTTLGMSIEPPKLQLQLQQHHDEEGQLHKKSSNNSNGNCAGGSHKPEANTTR
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map