TFome Information for pUT3188

TFome Name:pUT3188
Genbank Insert ID:JX469930
Protein Name:ZmbZIP37
Species: Maize
TF Family: bZIP
Template: BT036032.1
Corresponding TF Gene: GRMZM2G088140
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: B474_F 3' Primer Name: B474_R
5' Primer Sequence: caccATGGGAAGCAGTGGAGCAGAT 3' Primer Sequence: GTTTCCTTTTTTCTGGCTGTCATCACC
5' Primer Tm (º C): 69.3 3' Primer Tm (º C): 65.7
PCR Condition: PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGGAAGCAGTGGAGCAGATACACCGTCTAAGGAGCAGCAGCCACCTGCTACCTCAGGTGCTGCAATACCACCTGTTTA
TCCAGATTGGTCTAGCTTCCAGGCATATCCCCCAATCCCACCACATGGATTCTTCCCCTCACCTGTGGCATCAAGTCCAC
AGGGTCATCCTTTCATGTGGGGAGCTCAAGCTATGATTCCACCTTATGGAACACCGCCACCATATGTCATGTACCCCCCT
GGAGTATATGCCCACCCATCTATGCCCCCGGGTGCACATCCATTTACTCCTTATGCCATTACTTCTCCAAATGGCAATGC
TGATGCTACTGGAACTACTGTTGCAGCTGGTAATACTGATGGCAAGCCCTCTGAAGGCAAAGATAAAAGTCCAACAAAGC
GATCCAAAGGAAGTTTGGGCAGCTTGAACATGCTTACTGGAAAGAATCCCAGTGAACATGGTAAGTCCTCAGGTGCATCG
GGTAATGGAGTCACTTCTCAAAGTGGCGAAAGTGGCAGTGACAGTTCAAGCGAAGGGAGTGAAGGAAATTCTCACAATGA
TTCACATCACAAGGAAAGTGGACAGGAACACGATGGGGATGTTCGAAGTTCCCGGAATGGTGTATCACGTTTGCAATCTG
AGGGAAAATTGAACCAAGCTATGGCAATCTTGCCCATACCATCAAGTGGCCCAGCAACTGATCCAACTACAAACCTAAAT
ATTGGGATGGACTATTGGGCCAACACAGCAAGCTCTGCTCCTGCAATTCATGGCAAAGCGACGTCAACAACAGTTCCAGG
GGCTGTGGTCCCAGCAGAGCAGTGGACACAGGATGAACATGAACTCAAAAAGCAAAGAAGAAAGCAGTCCAATAGGGAGT
CTGCACGTAGATCTAGGTTACGTAAACAGGCTGAATGTGAGGAATTGGCTCAACGTGCTGATGTTTTAAAGCAGGAAAAT
GCTTCACTTAGAGATGAAGTAAACCGTATCAGAAAAGAGTACGAGGAACTTCTATCAAGGAATAACTCACTAAAGGAAAA
ACTTGAAGGCAAACAACACAAAACTGATGAGGCAGGACTTAACAACAAGCTGCAACATTCTGGTGATGACAGCCAGAAAA
AAGGAAAC
TFome clones do not contain stop codon at 3'end.
Translation: MGSSGADTPSKEQQPPATSGAAIPPVYPDWSSFQAYPPIPPHGFFPSPVASSPQGHPFMWGAQAMIPPYGTPPPYVMYPP
GVYAHPSMPPGAHPFTPYAITSPNGNADATGTTVAAGNTDGKPSEGKDKSPTKRSKGSLGSLNMLTGKNPSEHGKSSGAS
GNGVTSQSGESGSDSSSEGSEGNSHNDSHHKESGQEHDGDVRSSRNGVSRLQSEGKLNQAMAILPIPSSGPATDPTTNLN
IGMDYWANTASSAPAIHGKATSTTVPGAVVPAEQWTQDEHELKKQRRKQSNRESARRSRLRKQAECEELAQRADVLKQEN
ASLRDEVNRIRKEYEELLSRNNSLKEKLEGKQHKTDEAGLNNKLQHSGDDSQKKGN
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map