TFome Information for pUT3195

TFome Name:pUT3195
Genbank Insert ID:JX469936
Protein Name:ZmbZIP88
Species: Maize
TF Family: bZIP
Template: BT068393.1
Corresponding TF Gene: GRMZM2G425920
Transcript: T02
Vector: pENTR/SD/D-TOPO
Note: c.G187T p. G63W Not present in template FlcDNA. Not present in functional domain.

5' Primer Name: B504_F 3' Primer Name: B504_R
5' Primer Sequence: caccATGGCACCCCAGGGGCG 3' Primer Sequence: CTTGCGCCAAGTAGAGCCACACTCCA
5' Primer Tm (º C): 72.8 3' Primer Tm (º C): 70.7
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCACCCCAGGGGCGGAGAGCGACGAGGAGATACGGCGGGGTGCCAGAGATGGGCGGCGCGTCGGCGTCGGCCTCGTC
GGGCGCCGGCGCGGACGAGCGTCCCAAGGGGGAGGACGGCAAGCAGGGGCAGGTGGCGGCGGGGGCGCAGCCTCCAGCGG
GCGGGAAGAAGCGCGGGCGCACGGCGTGGGACAAGGAGCAGAACCGGCTGAAGCGGCTGCTGCGGAACCACGTGTCCGCG
CAGCAAGCGCGGGAGCGGAAGAAGGCGTACCTGACGGAGCTGGAGGCGAAGGCCAAGGGTCTGGAGCTCCGCAATGCGGA
GCTGGAGCAGCGGGTGTCCACGCTCCAAAACGAGAACAACACGCTCCGCCAGGTTATAGATGTTAGTGGAAAAGAATTCA
AATTCACCTGGGCATCTGGACGTGGCGAATTGTGTGATATTTATGAAGAACGCCAGAGTGGAGAGGATGGAAATGGGTTG
CTTCTGGAGTGTGGCTCTACTTGGCGCAAG
TFome clones do not contain stop codon at 3'end.
Translation: MAPQGRRATRRYGGVPEMGGASASASSGAGADERPKGEDGKQGQVAAGAQPPAGGKKRGRTAWDKEQNRLKRLLRNHVSA
QQARERKKAYLTELEAKAKGLELRNAELEQRVSTLQNENNTLRQVIDVSGKEFKFTWASGRGELCDIYEERQSGEDGNGL
LLECGSTWRK
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map