TFome Information for pUT3200

TFome Name:pUT3200
Genbank Insert ID:JX469940
Protein Name:ZmbZIP25
Species: Maize
TF Family: bZIP
Template: BT042450.1
Corresponding TF Gene: GRMZM2G080731
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B532_F 3' Primer Name: B532_R
5' Primer Sequence: caccATGGCTTATGCAAGCCCC 3' Primer Sequence: TTCCCGTGGTCGTGCGATC
5' Primer Tm (º C): 67.1 3' Primer Tm (º C): 65.7
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCTTATGCAAGCCCCGGGACTGATGATACGTCGACAGATCTAGATACTGACGAGAAGAATCACATGTTGGAGCTAGG
GCAGCTTGTGTCCCTTACAGCTTCTGATTCCGGCAACAAAGCAAAGGACAAGCTACTAGGTCAAAAGGCACTGCGCCGTC
TGGCCCAGAACCGTGAAGCTGCTAGGAAAAGCCGTCTAAGAAAGAAGGCATACGTTGAGCAACTTGAGAATAGCAGGCTG
AAACTTAGCCAGCTGGAGCAGGAACTCCAGCGTGCTCGCCAACAGGGCATCTTCATTCCTACTCCGGGAGACGACCAGCA
GCCAAACTCTACTAGTGAAAAAGGTGCTTTGGCGTTTGACAAGGACTACGCTGGATGGGAAGACGAGCACAGGAAGCAGA
TAAGCGAGCTGAGGGCCGCGCTCAGCGCACACGCCGGTGACGACGAGCTCCGGCGCATCGTGGACGGCGTCATGGCGCAT
CACCACGAAGCGTTCAGGCTCAAGTGCGTGGCAGCCAGGGCCGACGCCTTCCACGTGCTGTCGGGGATGTGGAAGACCCC
CGTGGAGAGGTGCTTTATGTGGCTGGGCGGATTTCGACCGTCAGAGATTCTAAAGTTCCTCGCAAGCCATCTGGACCCCC
TCACCGAGCGGCAGCTCGCGAGCGTACACGGCCTGCAGCAGTCCTCGCAGCAAGCCGAAGAGGGCATCTCGCAGCGGGTG
CAAGCGCTGCAGCAGTCGGTCGCGGAGACCCTCGCGTCGTCAGGGTCCCCGTGCCTGCCTGTGCCTGCAGGTCGTGCTCC
TCCGCCTGGCGACGACGCCGCTCCAGACTGCTCGGGGACGATGGCGGCGGCGGTTGCGAAGCTTGTCGCTCTTGAAAGCT
TGGTGCATGAGGCTGACGGTCTGCGGAGGCAGACTCTTGAGCAGATGCAGCGCGTGCTGACCACCCGCCAGTCCGCGCGG
GCGCTGCTTGTGATCAGTGACTACCTTTCCCGGCTACGCGCTCTGAGTTCTCTCTGGATCGCACGACCACGGGAA
TFome clones do not contain stop codon at 3'end.
Translation: MAYASPGTDDTSTDLDTDEKNHMLELGQLVSLTASDSGNKAKDKLLGQKALRRLAQNREAARKSRLRKKAYVEQLENSRL
KLSQLEQELQRARQQGIFIPTPGDDQQPNSTSEKGALAFDKDYAGWEDEHRKQISELRAALSAHAGDDELRRIVDGVMAH
HHEAFRLKCVAARADAFHVLSGMWKTPVERCFMWLGGFRPSEILKFLASHLDPLTERQLASVHGLQQSSQQAEEGISQRV
QALQQSVAETLASSGSPCLPVPAGRAPPPGDDAAPDCSGTMAAAVAKLVALESLVHEADGLRRQTLEQMQRVLTTRQSAR
ALLVISDYLSRLRALSSLWIARPRE
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map