TFome Information for pUT3216

TFome Name:pUT3216
Genbank Insert ID:JX469954
Protein Name:ZmbZIP39
Species: Maize
TF Family: bZIP
Template: BT034289.1
Corresponding TF Gene: GRMZM2G479885
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C230_F 3' Primer Name: C230_R
5' Primer Sequence: caccATGTCGTCGTCGTCGCTGT 3' Primer Sequence: GTAGTGGAGCATGTGCGGGGC
5' Primer Tm (º C): 69.3 3' Primer Tm (º C): 68.5
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C for clones:
Nucleotide Sequence: ATGTCGTCGTCGTCGCTGTCTCCGACGGCGGGGAGGACGTCCGGGTCGGACGGCGACTCGGCGGCGGACACGCACAGGCG
CGAGAAGCGCCGGCTGTCCAACCGCGAGTCGGCGCGGCGGTCTCGGCTCCGGAAGCAGCAGCACCTGGACGAGCTGGTGC
AGGAGGTGGCGCGCCTGCAGGCCGACAACGCGCGCGTGGGGGCCCGCGCCGCCGACATCGCGTCCCAGTACACCCGCGTC
GAGCAGGAGAACACCGTGCTCCGGGCCCGCGCCGCCGAGCTCGGCGACCGCCTCCGCTCCGTCAACGAGGTGCTCCGCCT
CGTCGAGGAGTTCAGCGGCGTCGCCATGGACATCCAGGAGGAGATGCCGGCGGACGACCCGCTGCTGCGTCCCTGGCAGC
TGCCCTACCCGGCTGCCGCCATGCCCATCGGCGCCCCGCACATGCTCCACTAC
TFome clones do not contain stop codon at 3'end.
Translation: MSSSSLSPTAGRTSGSDGDSAADTHRREKRRLSNRESARRSRLRKQQHLDELVQEVARLQADNARVGARAADIASQYTRV
EQENTVLRARAAELGDRLRSVNEVLRLVEEFSGVAMDIQEEMPADDPLLRPWQLPYPAAAMPIGAPHMLHY
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map