TFome Information for pUT3221

TFome Name:pUT3221
Genbank Insert ID:JX469959
Protein Name:ZmbZIP94
Species: Maize
TF Family: bZIP
Template: BT067773.1
Corresponding TF Gene: GRMZM2G007063
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.C128T p.A43V Not present in template FlcDNA. Not in functional domain.

5' Primer Name: C291_F 3' Primer Name: C291_R
5' Primer Sequence: caccATGGAGCGCGTCTTCTCCAT 3' Primer Sequence: GGAGGTCGAGCCGGATGAAGC
5' Primer Tm (º C): 69.3 3' Primer Tm (º C): 67.6
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGAGCGCGTCTTCTCCATGGAGGAGATCCCCAACCCCTACTGGGCCCCGCCGCACCCTCAACCGGCGGCCGGCGGCGC
TGTTGCTGCACCAGGTGGAGTAGGAGGAGCGGGGGACGCGGCGGGCGTGATGAACCGGTGCCCATCTGAGTGGTACTTCC
AGAAGTTCCTCGAGGAGGCCGTGCTCGACAGTCCGGGTCCCGTCGCCGGCGTGGGTAGAAGCAGCGGACAAGCTGGAGTT
GAGGCGGCGGAGAGCAAGCCGCTGGGCGCCGCGGCGCCGGCGTCCGTCTCGAGCTCGGTCGTTGACCCCGTCGAGTACAA
CGCGATGCTCAAGCAGAAGCTGGAGAAGGACCTCGCTGCCATCGCTATGTGGAGGGCTTCTGGTGCAGCACCTCCAGATC
TTTCTGCGACTGCTGCTTCCTTGCCAAGTGTCGGTGCTCCGCATGCAGCTCCTCTTAAACCCGTCGGAGGTACTGAAAGT
CTAGTTCAAAACATGCTAGCTGGTGCTCCAGTAGGGGGGTCAGGTCCACATGTAGTACAAATTGCTGATATCCCTGTTAA
GCAAACCACTAGCTCTTCCTCACGAGAGCAGTCAGATGATGATGACATGGAAGGCGATGCTGAGACAAATGGAAATGGAA
ACCCTGTTCAACAAAGACAACAGAGAAGGAAGCAATCCAACCGGGAATCAGCCAGGCGTTCGAGAAGCAGAAAGGCGGCT
CACTTGAATGAACTGGAGGCACAGGTAGCACAATTAAGAGTCGAAAACTCTTCGCTGCTAAGGCGGCTTGCTGATGTTAA
CCAGAAGTTCAATGAAGCTGCTGTTGACAATAGGGTGCTAAAGGCAGACGTCGAAACCTTAAGAGCAAAGGTGAAGATGG
CAGAGGACTCGGTGAAGCGGGTAACAGGCATGAACGCATTGTACCCTGCCGTGTCGGATATGTCTTCCCTCAGCATGCCA
TTCAATGGCTCCCCTTCTGACTCCGCCTCTGATAGCACCGTCCCGGTACAAGATGACCTGAACAGTTACTTCGCCAACCC
AAGCGAAATCGGATGCAACAACGGCTACATGCCAGATATAGCCTCCTCGGTTCAACAGGACGACAATTTTGTCAACGGGT
ATCAGGCTGCTGGCAAGATGGGCAGAACAGACTCGCTGCAGCGGGTGGCAAGCCTGGAGCACCTCCAGAAAAGAATGTGC
GGAGGCCCGGCTTCATCCGGCTCGACCTCC
TFome clones do not contain stop codon at 3'end.
Translation: MERVFSMEEIPNPYWAPPHPQPAAGGAVAAPGGVGGAGDAAGVMNRCPSEWYFQKFLEEAVLDSPGPVAGVGRSSGQAGV
EAAESKPLGAAAPASVSSSVVDPVEYNAMLKQKLEKDLAAIAMWRASGAAPPDLSATAASLPSVGAPHAAPLKPVGGTES
LVQNMLAGAPVGGSGPHVVQIADIPVKQTTSSSSREQSDDDDMEGDAETNGNGNPVQQRQQRRKQSNRESARRSRSRKAA
HLNELEAQVAQLRVENSSLLRRLADVNQKFNEAAVDNRVLKADVETLRAKVKMAEDSVKRVTGMNALYPAVSDMSSLSMP
FNGSPSDSASDSTVPVQDDLNSYFANPSEIGCNNGYMPDIASSVQQDDNFVNGYQAAGKMGRTDSLQRVASLEHLQKRMC
GGPASSGSTS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map