TFome Information for pUT3223

TFome Name:pUT3223
Genbank Insert ID:JX469961
Protein Name:ZmbZIP15
Species: Maize
TF Family: bZIP
Template: BT033858.1
Corresponding TF Gene: GRMZM2G402862
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C296_F 3' Primer Name: C296_R
5' Primer Sequence: caccATGGCGGCCAACTACCAC 3' Primer Sequence: GAATTGGGTGGATGAGGTTCTCCG
5' Primer Tm (º C): 68.6 3' Primer Tm (º C): 65.8
PCR Condition: PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCGGCCAACTACCACCACTACCAGATGGCGGTGCACGCGGCGGCGGCGGCGGCGGCGTGGAGGAGGGAGCCGGACAG
CCCGCAGCTGAGCTTCGTGAGCGGGTGCAGCTCCCTCTTCTCCATCTCCACGCTGCAGGACGACGACGACGACCGAGCCG
CCGCCGTCGTCATCGCCGCCGGCCACGCGCTGCCCTCCACGCCCGTCTCGCTCGCGGGCTTCGTCGGCGACGAGGTCGAC
ATGGAGGTGCAGCAGGCCAGCGGCGACGATCGGAGGAGCATCAGGATGATGAGGAACCGGGAGTCCGCGCTCCGCTCCAG
GGCCAGGAAGAGGGCATACGTGGAGAACCTGGAGAAAGAGGTTCGCCGGCTAGTGGATGACAACTTGAAGCTCAAGAAGC
AGTGCAAAGAGCTGAAACGGGAGGTAGCGGCACTGGTCCTCCCAACCAAGAGCTCACTTCGGAGAACCTCATCCACCCAA
TTC
TFome clones do not contain stop codon at 3'end.
Translation: MAANYHHYQMAVHAAAAAAAWRREPDSPQLSFVSGCSSLFSISTLQDDDDDRAAAVVIAAGHALPSTPVSLAGFVGDEVD
MEVQQASGDDRRSIRMMRNRESALRSRARKRAYVENLEKEVRRLVDDNLKLKKQCKELKREVAALVLPTKSSLRRTSSTQ
F
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map