TFome Information for pUT3229

TFome Name:pUT3229
Genbank Insert ID:JX469967
Protein Name:ZmABI40
Species: Maize
TF Family: ABI3-VP1
Template: BT035540.2
Corresponding TF Gene: GRMZM2G328742
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2771up 3' Primer Name: UT-2771dn
5' Primer Sequence: caccATGGCCACGAACCATCTCTCC 3' Primer Sequence: AATCTCCTCGTCATGCTGGTATGG
5' Primer Tm (º C): 63.4 3' Primer Tm (º C): 58.5
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGCCACGAACCATCTCTCCCAAGGGCAGCACCAGCACCCGCAGGCCTGGCCCTGGGGCGTGGCCATGTACACCAACCT
ACACTACCACCACCAGCAGCACCACCACTACGAGAAGGAGCACCTGTTCGAGAAGCCGCTGACGCCGAGCGACGTGGGCA
AGCTCAACAGGCTGGTGATCCCCAAGCAGCACGCCGAGAGGTACTTCCCTCTCAGCAGCAGCGGCGCCGGCGACAAAGGC
CTCATCCTGTGCTTCGAGGACGACGACGACGACGAGGCTGCCGCCGCCAACAAGCCGTGGCGGTTCCGCTACTCGTACTG
GACCAGCAGCCAGAGCTACGTGCTCACCAAGGGCTGGAGCCGCTACGTCAAGGAGAAGCAGCTTGACGCCGGCGACGTCG
TGCGCTTCCAGAGGATGCGTGGTTTCGGCATGCCCGACCGCCTGTTCATCAGCCACAGCCGCCGCGGCGAGACTACTGCT
ACTGCTGCAACAACAGTGCCCCCCGCTGCTGCTGCCGTGCGCGTAGTAGTGGCACCTGCACAGAGCGCTGGCGCAGACCA
CCAGCAGCAGCAGCAGCCGTCGCCTTGGAGCCCAATGTGCTACAGCACATCAGGCTCGTACTCGTACCCCACCAGCAGCC
CAGCCAATTCCCAGCATGCCTACCACCGCCACTCAGCTGACCATGACCACAGCAACAACATGCAACATGCAGGAGAATCT
CAGTCCGACAGAGACAACAGGAGCTGCAGTGCAGCTTCGGCACCGCCGCCACCGTCGCGGCGGCTCCGGCTGTTCGGCGT
AAACCTCGACTGCGGCCCGGGGCCGGAGCCGGAGACACCAACGGCGATGTACGGCTACATGCACCAAAGCCCCTACGCTT
ACAACAACTGGGGCAGTCCATACCAGCATGACGAGGAGATT
TFome clones do not contain stop codon at 3'end.
Translation: MATNHLSQGQHQHPQAWPWGVAMYTNLHYHHQQHHHYEKEHLFEKPLTPSDVGKLNRLVIPKQHAERYFPLSSSGAGDKG
LILCFEDDDDDEAAAANKPWRFRYSYWTSSQSYVLTKGWSRYVKEKQLDAGDVVRFQRMRGFGMPDRLFISHSRRGETTA
TAATTVPPAAAAVRVVVAPAQSAGADHQQQQQPSPWSPMCYSTSGSYSYPTSSPANSQHAYHRHSADHDHSNNMQHAGES
QSDRDNRSCSAASAPPPPSRRLRLFGVNLDCGPGPEPETPTAMYGYMHQSPYAYNNWGSPYQHDEEI
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map