TFome Information for pUT3236

TFome Name:pUT3236
Genbank Insert ID:JX469973
Protein Name:ZmABI3
Species: Maize
TF Family: ABI3-VP1
Template: BT043138
Corresponding TF Gene: GRMZM2G149940
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.G810T p.E270T. In template FlcDNA. Not in functional domain.

5' Primer Name: UT-2968up 3' Primer Name: UT-2968dn
5' Primer Sequence: caccATGGCCAACGCCAATGGCTC 3' Primer Sequence: CGTGATGATCTTCTTATTGATCGAAGGAC
5' Primer Tm (º C): 61.4 3' Primer Tm (º C): 57
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGCCAACGCCAATGGCTCGTCCACCGGTGCAGGCCATAGCGACCTCGTCAGGGCCATTTCGCACGAGCAGCACCAGGC
GTTCATGGCCTCCGTGCCCCGGGCCGCACCGGGCGGGGTGAATGTGCACCACCAGCAGCACTTCCACCAATACCCCGCCG
GCTTGATCCCAGCGCCGGTGGCACTGCCGGTTCACGCACCGGTGTCGTCACAGACGTCTCCGTACTCCGCCCAGATCGCG
GTGCCGCCGCCGCCGCCACCACTCATCGCATCTCCAGACCACCGCCTGCACTCCCTGCCGCCTACCGGATGCTACCAGCT
GGACTATTCTCCATATGGCAACGCTGCTGCGCCTTCGCAGCAGCACACATCAGCAATAAGGGGTTTTGCTGACTGGGGAA
CGCACAGCAATGCGCTCATGTCCTTGGCTCATGCCACTAGTTTTGGTAACAACGGGAGCAGCAATATCAACAACAATGGA
TTATTACATCAAAACTTATCCCCGTACACCACGCATACATGGACTACTACTTACGTGCAGAGGCCTTATAACACTGCTGT
TTATGCTCCTGCAACAATGAATATGCTCCAAACTCCTCCATTTCATAGCAACAGCCATGAAAAAGAGTCAGGTGCAGTTT
TTTCAAATAGCTTTAACATGGCCCCATCAGTGACACCTACCTCCCCTTTTCAACTTATGTCTCCAAGCTCAACCAACTAC
ACTTCCACTCAAATCTTCGAGGAAACCAACAATTTAGAGGACACTTCAAGAGTCTTTGGAGGTGGGGATAACGAAAGCAA
CAACAGTGATGAACCGGATCCCAAGCCGGCTGTGGAGATGGAAGACCTAAACCAAGGCAATGATCATACTTCGAATAAAA
CAGCTAACTGTCAAGATTACCGTATGGTCCTGCGCAAGGACTTGACAAACAGTGATGTTGGAAACATTGGAAGAATCGTG
CTGCCAAAGAAAGATGCAGAGCCTAACCTTCCAATCTTGGAAGATAAGGATGGCCTGATACTGGAAATGGACGATTTTGA
ACTCCCGGTTGTATGGAATTTCAAGTACAGGTACTGGCCTAATAACAAGAGTAGGATGTATATATTGGAAAGTACTGGTG
AATTTGTTAAGAGGCATGGGCTTCAAGCTAAGGACATCCTCATCATTTACAGGAACAAGAAATCTGGCAGATATGTTGCC
CGTGCGGTGAAGGCTGAGGACATAGCGCCGCCAGAATGCGAGTGCGTCGAGGCAGGAAACCCTAGAGAAGAGTGCGGCTT
CTCCGTCAGTCCTTCGATCAATAAGAAGATCATCACG
TFome clones do not contain stop codon at 3'end.
Translation: MANANGSSTGAGHSDLVRAISHEQHQAFMASVPRAAPGGVNVHHQQHFHQYPAGLIPAPVALPVHAPVSSQTSPYSAQIA
VPPPPPPLIASPDHRLHSLPPTGCYQLDYSPYGNAAAPSQQHTSAIRGFADWGTHSNALMSLAHATSFGNNGSSNINNNG
LLHQNLSPYTTHTWTTTYVQRPYNTAVYAPATMNMLQTPPFHSNSHEKESGAVFSNSFNMAPSVTPTSPFQLMSPSSTNY
TSTQIFEETNNLEDTSRVFGGGDNESNNSDEPDPKPAVEMEDLNQGNDHTSNKTANCQDYRMVLRKDLTNSDVGNIGRIV
LPKKDAEPNLPILEDKDGLILEMDDFELPVVWNFKYRYWPNNKSRMYILESTGEFVKRHGLQAKDILIIYRNKKSGRYVA
RAVKAEDIAPPECECVEAGNPREECGFSVSPSINKKIIT
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map