TFome Information for pUT3352

TFome Name:pUT3352
Genbank Insert ID:JX469992
Protein Name:ZmARF36
Species: Maize
TF Family: ARF
Template: BT065284.1
Corresponding TF Gene: GRMZM2G702026
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2783up 3' Primer Name: UT-2783dn
5' Primer Sequence: caccATGGCGGAGGCCGGCGTGGC 3' Primer Sequence: GTCCTTATCAGCAGCAACGGGAGCACTGTT
5' Primer Tm (º C): 72.8 3' Primer Tm (º C): 65
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 65C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGGCGGAGGCCGGCGTGGCACGCGGCTCAGGGAGTGCTGGCGATGCTTTGTTCAGGGAACTCTGGCATGCTTGTGCTGG
GCCGTTGGTCACAGTGCCTCGCCAAGGCGAGCTGGTTTATTACTTCCCGCAGGGTCATATGGAACAGCTTGAAGCGTCTA
CAGATCAACAACTTGATCAGCACTTGCCTTTGTTTAATCTACCGCACAAGATCCTCTGCAAGGTGGTCAATGTAGAACTT
AGAGCTGAAACTGATTCTGATGAAGTTTATGCTCAAATTATGCTGCAACCACAAACAGAGCAAAGTGAGCCCACCAGCCC
AGATCCTGAACCACCTGAGCCTGAAAGATGCAACATTCATTCCTTCTGCAAGACCTTGACTGCTTCAGATACAAGTACCC
ATGGTGGATTCTCTGTCCTCAGGAGGCATGCTGAAGAATGTTTGCCCCAACTGGACATGACTCAGAATCCACCGTGGCAA
GAACTAGTGGCTAAAGATCTCCATGGAAATGAATGGCATTTCCGTCACATCTTTCGAGGGCAACCAAGGAGGCATCTACT
TACAACAGGCTGGAGTGTTTTTGTTAGCTCAAAAAGATTGGTTGCGGGTGACGCGTTTATCTTCTTGAGAGGTGAGAACG
GAGAGCTGCGAGTCGGGGTAAGGAGGCTCATGAGACAACTAAATAACATGCCGTCTTCGGTTATCTCAAGCCACAGCATG
CATCTTGGAGTCCTAGCAACTGCATCTCATGCCATCTCCACTGGAACTCTCTTTTCTGTTTTCTACAAACCCAGAACAAG
TAGATCTGAATTTGTCGTTAGTGTAAACAAGTACCTCGAAGCTAAGAATCACAAGATGTCTGTTGGTATGAGGTTTAAAA
TGAGATTTGAGGGTGATGAATCCCCTGAAAGAAGATTCAGTGGGACAATTATTGGTATGGGATGCATGCCAGCTAACTCA
ACATCTCCATGGGCTAACTCTGAATGGAGATCATTGAAGGTCCAATGGGACGAGCCTTCCGCTATTCTGCGTCCAGATAG
AGTTTCACCATGGGAAGTAGAACCCCTTGATCGAACAAATCCACAGCCACCTCAACCTCCTTTACGGAATAAGCGAGCAC
GGCCTCCTGCTTCACCTTCGATTGCTCCAGAACTTGCTCCAGTTTTTGGTTTTTGGAAGTCCCCAGCTGAGCCTGCCCAA
GCTTTCTCATTCTCAGGACTGCAGCGAACTCAGGAGCTATACCATTCAAGCCCCAGTTCAATGTTCTCATCGTCGTTGAA
TGTGGGATTCAATCCAAAGTACGAGGGTCCCACTCCAAACACCAACCATTTGTACTGGACAATGAGAGAGACAAGAACTG
AATCCTACTCCGCTAGTATCAACAAAGCTCCAACTGAAAAGAAGCAAGAATCCACTACTTCTGGCTGCAGATTGTTCGGT
ATCGAGATAGGTTCTTCTGCAGTGTCACCCGTGGTTACTGTTGCTAGCGTCGGGCATGATCCTCCGCCACCTGCTCTCTC
AGTAGACGCTGAATCTGACCAGCTGTCACAGCCGTCCCATGCCAACAAAGCAACGGATGCCCCAGCGGCAAGCAGCGATC
GCTCTCCTAACGAGACAGAGAGCCGGCAAGCCAGGAGCTGCACCAAGGTAATCATGCAAGGGGTGGCGGTTGGCCGGGCG
GTGGACTTGACGAGGCTAGATGGGTACGATGACCTCCGCCGCAAGTTGGAGGAGATGTTCGATATCCCGGGAGAGCTTTC
CGCCAGCCTCAAGAAATGGAAGGTGATCTACACAGATGACGAGGACGATATGATGCTGGTCGGGGACGATCCATGGAGTG
AATTTTGCAGGATGGTAAAAAGGATATACATTTATTCCTACGAGGAGGCCAAGTCTTTGACTCCCAAGGCGAAGCTGCCG
GCCATCGGTGGTGACACCGGCGTCAAGCCGGACCCAAGCAAACTGCCACCAGAATCCGACGTGCCACAGAGCGACTCCGA
CAACAGTGCTCCCGTTGCTGCTGATAAGGAC
TFome clones do not contain stop codon at 3'end.
Translation: MAEAGVARGSGSAGDALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFNLPHKILCKVVNVEL
RAETDSDEVYAQIMLQPQTEQSEPTSPDPEPPEPERCNIHSFCKTLTASDTSTHGGFSVLRRHAEECLPQLDMTQNPPWQ
ELVAKDLHGNEWHFRHIFRGQPRRHLLTTGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRLMRQLNNMPSSVISSHSM
HLGVLATASHAISTGTLFSVFYKPRTSRSEFVVSVNKYLEAKNHKMSVGMRFKMRFEGDESPERRFSGTIIGMGCMPANS
TSPWANSEWRSLKVQWDEPSAILRPDRVSPWEVEPLDRTNPQPPQPPLRNKRARPPASPSIAPELAPVFGFWKSPAEPAQ
AFSFSGLQRTQELYHSSPSSMFSSSLNVGFNPKYEGPTPNTNHLYWTMRETRTESYSASINKAPTEKKQESTTSGCRLFG
IEIGSSAVSPVVTVASVGHDPPPPALSVDAESDQLSQPSHANKATDAPAASSDRSPNETESRQARSCTKVIMQGVAVGRA
VDLTRLDGYDDLRRKLEEMFDIPGELSASLKKWKVIYTDDEDDMMLVGDDPWSEFCRMVKRIYIYSYEEAKSLTPKAKLP
AIGGDTGVKPDPSKLPPESDVPQSDSDNSAPVAADKD
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map