TFome Information for pUT3354

TFome Name:pUT3354
Genbank Insert ID:KJ726815
Protein Name:ZmARF25
Species: Maize
TF Family: ARF
Template: BT067605.1
Corresponding TF Gene: GRMZM2G116557
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT-2964up 3' Primer Name: UT-2964dn
5' Primer Sequence: caccATGCCGCCCGCAGCCATG 3' Primer Sequence: CTCGTTTGCGGCGGCGGC
5' Primer Tm (º C): 65 3' Primer Tm (º C): 66
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 65C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs), GC buffer (NEB), MgCl2 3.5 mM final concentration, 10% Glycerol, template 40pg/ul final concentration.
Nucleotide Sequence: ATGCCGCCCGCAGCCATGGCGCTGCCGTCCCAGGCCCCGTCTAACTCAGGGGATCCGCTGTACCCGGAGCTCTGGCGCGC
CTGCGCCGGCCCGCTCGTCACCGTCCCCCGCGTCGGCGACCTCGTCTTCTACTTCCCCCAGGGACACATCGAGCAGGTGG
AGGCGTCCATGAACCAGGTTGCCGGGAACCCGATGCGTCTCTACGATCTGCCTTCCAAGCTGCTCTGCCGCGTGCTCAAC
GTCGAGCTGAAGGCGGAGACCGACACCGACGAGGTGTACGCGCAGATCATGCTCATGCCGGAGCCCGAGCAAAACGATGT
GGCTGCCGAGAAGACGAGCTCTGGGTCTGCTGCGCCACCGAGGCCAGCGGTCAGGTCATTCTGCAAGACGCTCACCGCCT
CCGACACCAGCACACACGGCGGCTTCTCTGTCCTGCGCCGCCACGCTGACGAGTGCCTCCCTGCCCTGGATATGTCCCAG
TCGCCTCCCACTCAGGAGCTGGTGGCGAAGGATCTGCATGGCATGGAGTGGCGATTCCGCCACATCTTCCGCGGGCAACC
CAGGAGGCATCTCCTTCAGAGTGGTTGGAGTGTTTTTGTCAGTTCCAAAAGGCTTGTTGCTGGAGACGCCTTCATTTTCC
TCAGAGGAGAAAACGGTGAACTTCGAGTTGGTGTTAGGCGCGCTATGAGGCAGTTGTCTAATGTACCTTCTTCGGTTATA
TCTAGCCAAAGCATGCATCTCGGGGTTCTTGCGACTGCATGGCACGCTATTAACACAAAGTCCATGTTCACTGTCTACTA
CAAACCTAGGACGAGCCCTTCAGAGTTCATCATTCCATATGATCAATATATGGAGTCAGTAAAAAACAATTATTCTATTG
GGATGAGATTCAGGATGAGGTTTGAAGGGGAAGAGGCCCCCGAGCAGAGGTTTACTGGTACAATAGTTGGCTGTGAAAAT
CTTGACCCACTATGGCCTGATTCCAGTTGGAGATACTTGAAGGTGCGCTGGGATGAGCCTTCTACTATCCCGCGGCCAGA
TAAGGTCTCTCCCTGGAAGATAGAGCCTGCTTCATCACCTCCTGTTAATCCTCTCCCTCTTTCTAGAGGAAAAAGACCTA
GACAAAATGCACCTCCACCTTCCCCTGAATCATCTGTTCTCACAAAAGAAGGTGCAACCAAGATTGATACTGATTCTGCT
CAAACACCACATCAAAATTCGGTCTTGCAAAGTCAAGAGCAGATGAGCTTTAGGAACAACCTGACTGAAAGCACCGACTC
TGATTCCACTGTTCAGAAGCAGATGATGTGGTCCCCATCACCCAATGGGAAAGTTCACACTAATTTTCAGCCGAGACCTG
CTATGGATAACTGGATGCCATTGGGAAGGCGTGAAACTGACTTCAAGGACACCCGTTCTGCCTTCAAGGATGCCCGCACC
GCTTCTCAATCGTTTGGAGATACACAGGGGTTCTTTATGCAAGCTTATGATGACAGTCGTCACCGTCTTTCTTTCAACAA
TCAGTTTCAAGATCAGGGTTCAGCTCATCGCTTTGCAGATCCATACTTCTATATGCCTCAACAACCTTCTCTGACGGTTG
AATCAGGCACAAGGACGCAAACAGCGAACAATGACTTGCGTTTTTGGAGTGAGCGGAATTCGATGTATGGTAATCCAAGT
GACCAACAGCAGGGTTTCAGCTTTGGGCAAAACACATCAAGTTGGTTGAACCAGCCACTTCCCCAGGTTGAACAGTCACG
AGTGGTCAGGCCGCATGCAACGGTTGCTCCATTTGATTTAGAGAAGACTAGAGAAGGCAGTGGGTTCAAAATATTTGGTT
TCCAAGTTGATACAACCAATCCATCACCTGTCCAATTGAGCTCTCCATTGTCTGCTATCCGAGAGCATGTGGTACAAACT
CGACCATCGGCACCAGTGAATGAATTGCAACCTGTGCAAATTGAGTGCTTGCCTGAGGTGTCCGTAAGCACAGCTGGAAC
TGCAGCTGAGAACATTCAGCAAGTCCAGCAAAGTTCGAAAGATATTCAAAGCAAGTCCCAGGGCGCTTCAACAAGGAGCT
GTACAAAGGTTCATAAGCAAGGAGTTGCGCTTGGCCGGTCCGTGGACCTCTCTAAATTCACCGACTACGGTGAGCTCAAA
GCGGAACTAGACAAGATGTTTGAGTTCGAGGGCGAATTGGTATCTGCTAACCGGAACTGGCAGATCGTTTATACCGACAA
CGAGGGTGATATGATGCTTGTCGGAGATGACCCGTGGGAGGAGTTCTGCAACATAGTGCGCAAGATCTACATCTACACGA
AGGAGGAGGTCCAGAAGATGAACTCGAAATCATCCGTGCCGAGGAAGGAGGAACCCCCAGCAGCAGGCGAAGGCTGCGCC
GCCGCCGCCGCAAACGAG
TFome clones do not contain stop codon at 3'end.
Translation: MPPAAMALPSQAPSNSGDPLYPELWRACAGPLVTVPRVGDLVFYFPQGHIEQVEASMNQVAGNPMRLYDLPSKLLCRVLN
VELKAETDTDEVYAQIMLMPEPEQNDVAAEKTSSGSAAPPRPAVRSFCKTLTASDTSTHGGFSVLRRHADECLPALDMSQ
SPPTQELVAKDLHGMEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQLSNVPSSVI
SSQSMHLGVLATAWHAINTKSMFTVYYKPRTSPSEFIIPYDQYMESVKNNYSIGMRFRMRFEGEEAPEQRFTGTIVGCEN
LDPLWPDSSWRYLKVRWDEPSTIPRPDKVSPWKIEPASSPPVNPLPLSRGKRPRQNAPPPSPESSVLTKEGATKIDTDSA
QTPHQNSVLQSQEQMSFRNNLTESTDSDSTVQKQMMWSPSPNGKVHTNFQPRPAMDNWMPLGRRETDFKDTRSAFKDART
ASQSFGDTQGFFMQAYDDSRHRLSFNNQFQDQGSAHRFADPYFYMPQQPSLTVESGTRTQTANNDLRFWSERNSMYGNPS
DQQQGFSFGQNTSSWLNQPLPQVEQSRVVRPHATVAPFDLEKTREGSGFKIFGFQVDTTNPSPVQLSSPLSAIREHVVQT
RPSAPVNELQPVQIECLPEVSVSTAGTAAENIQQVQQSSKDIQSKSQGASTRSCTKVHKQGVALGRSVDLSKFTDYGELK
AELDKMFEFEGELVSANRNWQIVYTDNEGDMMLVGDDPWEEFCNIVRKIYIYTKEEVQKMNSKSSVPRKEEPPAAGEGCA
AAAANE
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map