TFome Information for pUT3382

TFome Name:pUT3382
Genbank Insert ID:KJ726842
Protein Name:ZmCOL5
Species: Maize
TF Family: C2C2-CO-like
Template: BT061116
Corresponding TF Gene: GRMZM2G075562
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT2808up 3' Primer Name: UT2808dn
5' Primer Sequence: caccATGGCTTCTCTCTGTGATTTCTGTG 3' Primer Sequence: GTAGCTTCTAGTCTGGCTTAGCGG
5' Primer Tm (º C): 55.6 3' Primer Tm (º C): 55
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 60.9°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGCTTCTCTCTGTGATTTCTGTGGGAAACAAAGGTCAATGATCTACTGCAGATCAGATGCGGCATCATTGTGCTTATC
ATGCGACCGTAATGTCCATTCAGCTAATGCACTGTCTCGACGTCATACAAGGACCCTTCTTTGTGATCGTTGTGGTTCAC
AGCCTGCATCAGTCCGATGTCTTGAGGACAACGCATCACTTTGCCAAAACTGTGACTGGAATGGGCATGATGCAGAATCA
GGGGCTTCTGGACATAAAAGGCAAGCCATAAACTGCTACTCAGGGTGCCCATCATCTGCAGAGCTTTCGAGAATCTGGTC
ATTTATTACTGATATCCCGACTGTAGCAGCTGAGCCCGACTATGAGGATGGACTAAGCATGATGACAATTGATGGCAGTG
ATGTTACTAATCGTCACGATACTTCAGATGATAAAAGATTGTTGGAAATAGCTAACACAACACTCATGAGCGATCCACCT
TCAGCCGACAAGCTTAAATCTCCGACAGGCTCTTCTTCTGGAGATGGGTTTGATGTTATGACTCTAGCCACAGATCAGCC
TGCTGGACCAGTTTCAGCAACGCCTAAGGTACCTAATGCCAGAGATGATGATAAGTTCAATGATGGCATGTACGAAGACT
TGTGTGTAGATGATGCTGACCTTACATTCGAGGATTATGAAGAGCTATTTGGTACCTCTCACATTCGAACAGAGGAACTC
TTTGATGACGCTGGAATTGACGGTTACTTCGAATTGAAAGAGACACCACCTTTTTATTTCAACGAGCAGCCCAAAGCTAT
GCAGATAGAATGTGGCAACGTGGTATCAGCTGATTGTGCGATGTCAAACCCAGGGGCAAGGGCTGATTCCAGCCTTTGTA
TTCCCGTCAGGCAGGTCAGATCTAGTATATCCCACTCCTTATCTGGTCTGACTGGTGAGAGCAGCGCTGGAGATCACCAC
GACTGTGGGGTGTCACCGATGCTCCTCATGGGTGAGCCACCCTGGCATTCTCCTGGTGGTCCAGAAGGCTCAGTCGCTGG
AGGCAGCAGAGATAGCGCTCTCACACGGTACAAGGAGAAGAAGAAGAGGAGAAAGTTTGACAAGAAGATCAGATATGCTT
CTCGCAAGGCTAGGGCGGACGTGAGGAAGCGGGTCAAGGGACGGTTCATAAAGGCCGGTGAAGCGTACGACTATGATCCG
CTAAGCCAGACTAGAAGCTAC
TFome clones do not contain stop codon at 3'end.
Translation: MASLCDFCGKQRSMIYCRSDAASLCLSCDRNVHSANALSRRHTRTLLCDRCGSQPASVRCLEDNASLCQNCDWNGHDAES
GASGHKRQAINCYSGCPSSAELSRIWSFITDIPTVAAEPDYEDGLSMMTIDGSDVTNRHDTSDDKRLLEIANTTLMSDPP
SADKLKSPTGSSSGDGFDVMTLATDQPAGPVSATPKVPNARDDDKFNDGMYEDLCVDDADLTFEDYEELFGTSHIRTEEL
FDDAGIDGYFELKETPPFYFNEQPKAMQIECGNVVSADCAMSNPGARADSSLCIPVRQVRSSISHSLSGLTGESSAGDHH
DCGVSPMLLMGEPPWHSPGGPEGSVAGGSRDSALTRYKEKKKRRKFDKKIRYASRKARADVRKRVKGRFIKAGEAYDYDP
LSQTRSY
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map