TFome Information for pUT3383

TFome Name:pUT3383
Genbank Insert ID:KJ726843
Protein Name:ZmCOL14
Species: Maize
TF Family: C2C2-CO-like
Template: BT035398
Corresponding TF Gene: GRMZM2G042198
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT2764up 3' Primer Name: UT2764dn
5' Primer Sequence: caccATGGTTCCTCTCTGTGGTTTCTG 3' Primer Sequence: GTAGCTTCTAGTTTGACTTAGTGGATCATAG
5' Primer Tm (º C): 54 3' Primer Tm (º C): 54.8
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 60.9°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGTTCCTCTCTGTGGTTTCTGTGGGAAACAAAGGTCAATGATCTACTGCAGATCGGATGCGGCATCATTGTGCTTATC
ATGCGACCGTAGCGTTCATTCAGCTAATGCACTGTCTCGGCGTCATAGAAGGACCCTTCTTTGTGATCGTTGTGGTTTAC
AGCCTGCATCAGTCCGATGTCTTGAGGACAACACATCACTTTGCCAAAACTGTGATTGGAATGGGCATGATGCAGCATCA
GGGGCTTCTGGGCATAAAAGGCAGGCCATAAACTGTTACTCAGGGTGCCCATCATCAGCAGAGCTTTCGAGAATCTGGTC
ATTTATTATCGATATCCCGACTGTAGCTGCTGAGCCCAACTGTGAGGATGGGCTAAGCATGATGACAATTGATGACAGTG
ATGTGACTAATCATCACGGTGCTTCAGATGATAAACGATTGTTGGAAATAGCTAACACAGCACTCATGAGTGATCCACCT
TCACCTGACAAGCTTAAACCTCTGATAGGCTCTTCTTCTGGAGATGGGTTTGATGTTCTGCCTCTAGCCACAGATCAGCC
TGCTGGACCAGTTTCAGCGACACCTAAGGTACCTTATGCCAGAGATGACAATAAGTTCAATGATGGCATGTATGAAGACT
TATGTGTGGATGATGCTGACCTGACATTCGAGAATTATGAAGAGCTATTCGGTACCTCTCACATTCGAATAGAGGAACTC
TTTGATGACGCCGGAATTGACAGTTACTTTGAAATGAAGGAAACACCGCCTTTTGATTTCAATGAGCAGCCCAAAATTGT
GCAGCTACAATGTAGCGATGTGGTACCAGCTGATTGTGCGATGTCAAACACAGGGGAAAGGGCTGATTCCAGCCTTTGTA
TTCCTGTTAGGCAGGTCAGATCTAGTATATCCCATCCCTTATCTGGTTTGACTGGTGAGAGCAGCGCTGGAGATCACCAA
GACTGTGGGGTGTCACCAATTCTCCTCATGGGCGAGCCACCCTGGTATTCTCCTGGTCCAGAAGGCTCATTAGCTGGAGG
AAGCAGAGATAGTGCTCTCACACGATACAAGGAGAAGAAGAAGAAAAGAATGTTTGACAAGAAGATCAGATATGCTTCTC
GCAAGGCTAGGGCAGACGTGAGGAAGAGGGTCAAGGGACGGTTTATAAAGGCTGGCGAAGCGTATGACTATGATCCACTA
AGTCAAACTAGAAGCTAC
TFome clones do not contain stop codon at 3'end.
Translation: MVPLCGFCGKQRSMIYCRSDAASLCLSCDRSVHSANALSRRHRRTLLCDRCGLQPASVRCLEDNTSLCQNCDWNGHDAAS
GASGHKRQAINCYSGCPSSAELSRIWSFIIDIPTVAAEPNCEDGLSMMTIDDSDVTNHHGASDDKRLLEIANTALMSDPP
SPDKLKPLIGSSSGDGFDVLPLATDQPAGPVSATPKVPYARDDNKFNDGMYEDLCVDDADLTFENYEELFGTSHIRIEEL
FDDAGIDSYFEMKETPPFDFNEQPKIVQLQCSDVVPADCAMSNTGERADSSLCIPVRQVRSSISHPLSGLTGESSAGDHQ
DCGVSPILLMGEPPWYSPGPEGSLAGGSRDSALTRYKEKKKKRMFDKKIRYASRKARADVRKRVKGRFIKAGEAYDYDPL
SQTRSY
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map