TFome Information for pUT3398

TFome Name:pUT3398
Genbank Insert ID:KJ726857
Protein Name:ZmbHLH25
Species: Maize
TF Family: bHLH
Template: BT035033
Corresponding TF Gene: GRMZM2G081816
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3255up 3' Primer Name: UT3255dn
5' Primer Sequence: caccATGGATCATAACCTGAGCGACATGAC 3' Primer Sequence: TCCTGCTCCTCGTAGGGTTTCG
5' Primer Tm (º C): 62 3' Primer Tm (º C): 60.6
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59.5°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGATCATAACCTGAGCGACATGACGATCCTGAGCGAGCACGCATGGGGCGACGAGGCCGGCGCCGTCTTGCTGTCGCC
ACCGGAGGTGGGCAGCGTCATCGAAGGCGGCGGGGGCATGACGGTGCTGGAGAGGCTGGTGCTCGACGAGGCGCTGGCAG
CCGCGATCCTGGAGCTGCAGGCCATCCAGCAGGCCGCCCCCGGGCCCGCCTCCTGCGCCGGCAAGCAGCAGCTGGCGGCC
GGTGGCGGTGGTGGTGTGGTTGGGGCGGAGGCCGCTGTCGCCTTCCCCTCGATGATGGCGATGGCGATGGCGGCGCCAAC
GCCCGCCTACGCGGACGCGGACGTGGACGCGGGTGTCCTGCAGCGACAGCAGCATCACCATCGCCCTCACGCCGCGATGG
GGATGCCTAGTCCCGACTACGACCTCGCACCAGCAGCAGCAGCAACACCCGCGGTCGCGGTAACTGCTGCCGCGCCGCCC
GCCTTCACCACAAACACAAACGCCGCGGTTGACAGCGGATGCAGCGGCCTCGTAGTCCTTGGACGCTCGCCGCCGCCGCC
GGTCTTCTCCGCCAGCAACGAGACCGCAGCAACGGCGAGCCTGGACTGTGAACATGGCGGCGGAGGCCGTGGTGGTGGCC
GAAGGCAACGCCGGCCCAACAGGAAGAGGAAGGCCGCCGCCGAGGCCGAGCGGTCGCGACCTGTGCGTGTGGCGGCGGCA
GCGGCACAGGAAGAGAGCACCTTGTGCAGCCTCCTGGCGTCCACCGCCCCGACCACCGCGGGCGGCATCCAGATCGCGTT
CAGCACGGCGGCCGTGCATCAGCAGCAGCATGCTGCCGGTGGGGCCGGCGGCGCCAAGACATCAGCGAAGCCGTCGTCTC
TGAGTCTGAGCAGCAGCGGCTCGTCGAGCAACATCAGCTTCGACGGCCGGAACCCCGCGCGCAACGTGCGCATCTCGTCC
GACCCGCAGACGGTGGCGGCGCGGCAGCGGCGGGAGCGCATCAGCGACCGCCTCCGCGTGCTGCAGAAGCTCGTCCCGGG
CGGCGCCAAGATGGACACCGCCTCCATGCTGGACGAGGCCGCCAGCTACCTCCGCTTCCTCAAGTCCCAGGTCCGGGACC
TGCAAACCCTAGACCGCCGCAACTACGGTGCAGCTTCTTCGAATGACGCCGCCGCCACCATGGCTGCCGTTGTCGGGTCG
TCACTCAGCTACAACCGTGGCACCGGTGCCATGCCCGCATTCACCTTCCCGGCAGCAGGCGAAACCCTACGAGGAGCAGG
A
TFome clones do not contain stop codon at 3'end.
Translation: MDHNLSDMTILSEHAWGDEAGAVLLSPPEVGSVIEGGGGMTVLERLVLDEALAAAILELQAIQQAAPGPASCAGKQQLAA
GGGGGVVGAEAAVAFPSMMAMAMAAPTPAYADADVDAGVLQRQQHHHRPHAAMGMPSPDYDLAPAAAATPAVAVTAAAPP
AFTTNTNAAVDSGCSGLVVLGRSPPPPVFSASNETAATASLDCEHGGGGRGGGRRQRRPNRKRKAAAEAERSRPVRVAAA
AAQEESTLCSLLASTAPTTAGGIQIAFSTAAVHQQQHAAGGAGGAKTSAKPSSLSLSSSGSSSNISFDGRNPARNVRISS
DPQTVAARQRRERISDRLRVLQKLVPGGAKMDTASMLDEAASYLRFLKSQVRDLQTLDRRNYGAASSNDAAATMAAVVGS
SLSYNRGTGAMPAFTFPAAGETLRGAG
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map