TFome Information for pUT3399

TFome Name:pUT3399
Genbank Insert ID:KJ726858
Protein Name:ZmbHLH55
Species: Maize
TF Family: bHLH
Template: BT087345
Corresponding TF Gene: GRMZM2G030762
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3257up 3' Primer Name: UT3257dn
5' Primer Sequence: caccATGAACTGCGGGCCGCCC 3' Primer Sequence: AAGCTCCATTTTCATGTGGACTGTCTG
5' Primer Tm (º C): 67.9 3' Primer Tm (º C): 59.2
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 59.5°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGAACTGCGGGCCGCCCGACCAGCTGCCGCCGGGGTCGGCGCCGTCGTGCTTCCTCAACCTGAACTGGGACCAGTCTAT
GGCCGCCGACCAGCTCGACCCGGCGCTCAGCTCGATGGTCTCCTCCCCGGCGTCCAACTCGACGGCCGCCGCCGCGGTTA
CTGACGGCCGCGCTCTCCACGGGATCTCTCCGCAGCAGCAGTACGGAGGCACTCCGCTCAGCTCGCCCCCCAAGCTCAAC
CTGTTCCACCAAACTCGCCCCCAGTTCCACCACTTCCCGCCGCCGCAGGTCGGCGGCCTGCCAATCCTGGAGAACCTGAT
GCCGATGGGCCATCTAGACCAGTTCCTCGCCGACCCAGGCTTCGCCGAGCGCGCGGCGCGGCTGTCCGGCTTCGACGGCC
GCCCCGGTGGAAGTGGCTATGGAGGCGTCGGCGTCCCGGGACAGTTTGGCATCCCGGACGCCGGCCCCATCGGCGCATTG
AAGGAGCTGGAGCTCGGGAACGGCCGGGACGAGTCGTCGGTGTCCGATCCGGCGTCCGGCAGCGCGGAGATGGCGCTCAA
CAAGGGGCCTTCCGACGGCAATGCGAAGAAACGGAAGGCTAGCGGGAAGGGCAAAGGCAAGGACGGCCCCGGGTCCGCCG
CCGCCGGCGCCGCCAAGGAGGAGTCGAGTGGGAAGCGGTGCCGATCGGCGGACGAGAGCAGTGGCGCGGAGGACAACAAC
CCCACCACCAAGGGCAAGGCCGCGCAGAGCAGTAGCGAGAATGGTGGTGGCAGGAAGCAGCAGGGGAAGGAGAGCGCGAC
GAAGCCCCCCGCCGAGGCGCCCAAGGACTACATCCATGTCCGGGCGCGGCGCGGCGAGGCGACGGACAGCCACAGCCTCG
CGGAGAGGGTGAGAAGGGAGAAGATCAGCCAGCGGATGAAGCTGCTGCAGGATCTCGTGCCGGGTTGCAACAAGGTGGTG
GGCAAGGCAGTGATGCTGGACGAAATCATAAACTACGTGCAGTCCCTGCAACGGCAAGTCGAGTTCCTGTCCATGAAACT
GGCCACCGTGAACCCGCAGCTGGACTTCAACAGCCTGCCCAACCTCCTCCTCCCTAAAGACATACACCAGCCCTGTGGGC
CGCCGCATTTCCCGCTGGAGACCTCAGGCGCTCCGCTGCCGTACCTGAGCCAGCCTCACCATGGGAGCCCTCTAGGCTGC
TGCATGGACACCCAGGGGGGCTCTATGCACCCGCTCGACGCGGCGTTCTGCCGGCCGATGAACCCTCAGCATCCTTTCCT
CAACGGTGCTAGCGACGCGGCGTCTCAGGTCGGGACTTTCTGGCAAGACGACCTTCAAAGCGTGGTTCACATGGACATCG
GCCAAAGCCAGGAGATCGCTCCCACCTCTTCCAACAGCTACAACGGTTCATTGCAGACAGTCCACATGAAAATGGAGCTT

TFome clones do not contain stop codon at 3'end.
Translation: MNCGPPDQLPPGSAPSCFLNLNWDQSMAADQLDPALSSMVSSPASNSTAAAAVTDGRALHGISPQQQYGGTPLSSPPKLN
LFHQTRPQFHHFPPPQVGGLPILENLMPMGHLDQFLADPGFAERAARLSGFDGRPGGSGYGGVGVPGQFGIPDAGPIGAL
KELELGNGRDESSVSDPASGSAEMALNKGPSDGNAKKRKASGKGKGKDGPGSAAAGAAKEESSGKRCRSADESSGAEDNN
PTTKGKAAQSSSENGGGRKQQGKESATKPPAEAPKDYIHVRARRGEATDSHSLAERVRREKISQRMKLLQDLVPGCNKVV
GKAVMLDEIINYVQSLQRQVEFLSMKLATVNPQLDFNSLPNLLLPKDIHQPCGPPHFPLETSGAPLPYLSQPHHGSPLGC
CMDTQGGSMHPLDAAFCRPMNPQHPFLNGASDAASQVGTFWQDDLQSVVHMDIGQSQEIAPTSSNSYNGSLQTVHMKMEL
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map