TFome Information for pUT3401

TFome Name:pUT3401
Genbank Insert ID:KJ726860
Protein Name:ZmbHLH125
Species: Maize
TF Family: bHLH
Template: BT035360
Corresponding TF Gene: GRMZM2G017349
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3261up 3' Primer Name: UT3261dn
5' Primer Sequence: caccATGAACGAGCAGGGGCTCGG 3' Primer Sequence: CTCTTTGCTATGTGAGCAACCATCAAA
5' Primer Tm (º C): 65.9 3' Primer Tm (º C): 57.7
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57.8°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGAACGAGCAGGGGCTCGGTGGTCTCGGCGGGGGCCGCGGGGCCGTGCAGGGGCACGGGCGGGAGGCGATGGCGCTCTT
GCAGCACCAGCAGCTTCAACAGCAACGGCGGCAGCTGGAGGAGGAGGATGAGGTGCGGCGCCAGATGTTCGGCGGGGTGG
CGGCGTTCCCGGCAGCGCTGGGCCACGGCCAGCAGGTGGACTACGGGGAGGACGCCGGCGGGCTCGGGGACAGCGACGCG
GGCGGGTCGGAGCCCGAGCCACCGCCGGAGCGGACGCGCGGGGGGTCCGGCGGCGGCGGCGGCAAGCGGAGCCGCGCTGC
CGAGGTGCACAACCTCTCCGAGAAGAGGAGGAGGAGCAAGATCAACGAGAAGATGAAAGCGCTGCAGAGCCTGATACCCA
ACTCCAACAAGACCGACAAGGCATCCATGCTTGACGAGGCCATTGAGTATCTGAAGCAACTGCAGCTCCAAGTGCAGATG
CTGTCTATGAGAAATGGTGTTTACTTGAACCCTCCATATTTGTCTGGCACAATTGAGCCTGCACAAGCATCACAAATGTT
TGCTGCAGTTGGTGGGGGCAACATTACAGCGTCAAGCTCTGGGGCAGTGATGCCACCAGTAAACCAAAGTTCAGGACTTC
AAGTATTTGATCCATTAAATCCTCCACGAGACCAACCACTGTCTTTTGTATTACCAAATGTAGACAAAACCATTCAAGAA
GCTCCCTTTCACTTGGAATCATCGCAGTTCCACCCTCGACCATTTCGAATGCCTGAATCTTCTGAGATGATGCTTCCAGG
TGAGGTAGTGGCAAAGCACCAGCTAACTTCAATTCAAGGAAGAGTTAGTATGCCAGGAATTGGTATGAACCCCATTAGGC
AAGAATCATCCACTGTAAAAGCTGATCAGTTTGATGGTTGCTCACATAGCAAAGAG
TFome clones do not contain stop codon at 3'end.
Translation: MNEQGLGGLGGGRGAVQGHGREAMALLQHQQLQQQRRQLEEEDEVRRQMFGGVAAFPAALGHGQQVDYGEDAGGLGDSDA
GGSEPEPPPERTRGGSGGGGGKRSRAAEVHNLSEKRRRSKINEKMKALQSLIPNSNKTDKASMLDEAIEYLKQLQLQVQM
LSMRNGVYLNPPYLSGTIEPAQASQMFAAVGGGNITASSSGAVMPPVNQSSGLQVFDPLNPPRDQPLSFVLPNVDKTIQE
APFHLESSQFHPRPFRMPESSEMMLPGEVVAKHQLTSIQGRVSMPGIGMNPIRQESSTVKADQFDGCSHSKE
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map