TFome Information for pUT3402

TFome Name:pUT3402
Genbank Insert ID:KJ726861
Protein Name:ZmbHLH29
Species: Maize
TF Family: bHLH
Template: BT066715
Corresponding TF Gene: GRMZM2G101350
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3262up 3' Primer Name: UT3262dn
5' Primer Sequence: caccATGGCGCGGTGCCAGGGGAC 3' Primer Sequence: CATTTCCATTTTGAGGTTGTCTAGCAAGCAGC
5' Primer Tm (º C): 70.7 3' Primer Tm (º C): 61.4
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 62.0°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGCGCGGTGCCAGGGGACCGTTGAGAGCCTCTGCCAGGGGCTGCTCGACCTCGACGACGACAAGTTCGGTGCAATGTG
CAGCGCCTTCGGCTACCTCCAGGAGTGGCCGGACCTGGGTGCCGCCATGTGCGGCGCCAATCTCGCCGTGCCGCCTGCCG
TGGCGACGCCGAGCGCCGGCGACGGCAACGATTCCTCCTCTTGCAGCGTCAGCGGCGGGTTGAGGAAGAGGAGGCCGGAC
GCGTACCTCGATGCCAAGGGTGGTGCGAACGGCATGAACAAGAGACCCAGAGGGAAGCAGCAGCCGTCTGGCCTCTCTGA
GATCGCGGCGGCGCCCGGGAAAGGGAAGCAGGAGAGGCCAAAGGCTGGCACCAAGAAGAAGGCCGAGGCTGCGGCGGCGG
CGGCGGCTGCCGGCCAGAAGACCGACTACATCCACGTCCGGGCCCGCCGCGGCCAGGCCACGGACAGCCACAGCCTCGCC
GAGCGGGTGAGGAGGGAGAGGATCAGCGAGCGGATGCGGTACCTGCAGGAGCTGGTGCCCGGGTGCAGCAAGGTCACGGG
CAAGGCCGGCATGCTCGACGAGATCATCAACTACGTCCAGTCCCTGCAGAAGCAAGTCGAGTTCTTGTCCATGAAGATCG
CCGCTTCCAACCCGGTGGTGAGCTTCGACATCGTCGAGGACCTCTTCGGCCGCCAGCTGAAGCAGGCGTGCGGCCCGGCG
GCCGGCCAGCTGGAGCCGTCCTGCCTTCAGATGAGCATGAGCCCCATGCAGCAGGTAGTGCACCCGTCTGCAGCGGCGGG
CTCCACTGGCTTTGGGCCGCTGGACATGGTCCTCAGCAACCTGTACGCGCCCGCCGCCGCAAGCTGCCCTGTGTCGGCTG
CCCCGTCGGTCTCGGTGGCCGCCGGACCGTCGACCATCGAGCCGTGCCTCAACGTGAATGGAACTGCTGCTGCCTGGGAT
ATTGGCTCCCAGAATTTGTTCAGCGGATTTGATGCACAATTTCAGTCGGTAGAAAGTGGCTGCTTGCTAGACAACCTCAA
AATGGAAATG
TFome clones do not contain stop codon at 3'end.
Translation: MARCQGTVESLCQGLLDLDDDKFGAMCSAFGYLQEWPDLGAAMCGANLAVPPAVATPSAGDGNDSSSCSVSGGLRKRRPD
AYLDAKGGANGMNKRPRGKQQPSGLSEIAAAPGKGKQERPKAGTKKKAEAAAAAAAAGQKTDYIHVRARRGQATDSHSLA
ERVRRERISERMRYLQELVPGCSKVTGKAGMLDEIINYVQSLQKQVEFLSMKIAASNPVVSFDIVEDLFGRQLKQACGPA
AGQLEPSCLQMSMSPMQQVVHPSAAAGSTGFGPLDMVLSNLYAPAAASCPVSAAPSVSVAAGPSTIEPCLNVNGTAAAWD
IGSQNLFSGFDAQFQSVESGCLLDNLKMEM
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map