TFome Information for pUT3403

TFome Name:pUT3403
Genbank Insert ID:KJ726862
Protein Name:ZmbHLH173
Species: Maize
TF Family: bHLH
Template: BT035377
Corresponding TF Gene: GRMZM5G882527
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3263up 3' Primer Name: UT3263dn
5' Primer Sequence: caccATGGCGGGCTTCTCACGGC 3' Primer Sequence: CTGGAAAGAGCACATGTTGTTGAACA
5' Primer Tm (º C): 67 3' Primer Tm (º C): 58
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt,57.8°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGCGGGCTTCTCACGGCCGAACAACTCGCTCCTCCTCAAGATGCCTGCGACTTTGGCCAGTAGCCACACCCCGAACCT
CTCAAGCCTCCTGTTCTACGACCAGAACCATGGCCAAGCTGTAAATGCAAATGCAGCAGCGATGATGGTGGAGGACGACG
CCTCACTTCAGAGCTCATCTGCAGTGGTCGACACCTCCCCGCAGCCACAGGGCAGTGCTGCTGCTGCGTCTCCGATGGAG
AGGAAGAGGAAGCCTGCAGAAGACAGTGCTACACTCAGCTCTGCTCAATCCAAGGAGGATTGCAAGCAGCAGCAGGAGAG
CAAGAGCAGGCGGGGAAAGAGGCCCCACAACAAGGAGGCTAATACTGAGGAGAAGAGCGCTACTGAAGACGAGGCCACCA
GGGGGTACATCCATGTGAGGGCGAGGAGGGGTCAGGCAACGGACAGCCACAGCCTTGCAGAGAGGGTGAGGAGAGAGAGG
ATCAGTGAGAGGATGAGGATGCTGCAAGCACTGGTCCCTGGTTGTGACAAGGTTACTGGAAAGGCCCTGATTCTGGATGA
GATTATCAACTATGTGCAGTCCCTGCAGAACCAAGTTGAGTTCCTTTCCATGAGGATTGCCTCCATGAGCCCAGTGCTAT
ATGGGTTCGGACTGGATAGTGATGGCCTCCATGACCACGCACAAAAGATGGGAGTCATGTTCCAGGAAGCCCTTGCAATG
GCAGGTCCAGTGCTGAGCCAAGCCAGCCCAGCAGCTCCATCTGAAGCCATCATGGACACCACCACACCTTACTCACTGCA
TGGCCATGGCCAGGGTTCCATCTCTTTTTCCCAGGACAATGGCAGCAGCACTTACCTGATGCAGCATCAGCAAGCAGCGG
GGGAGCCAACAAGGCAGCAGGAGCTGCTCAGCCAGTTGGTGTTCAACAACATGTGCTCTTTCCAG
TFome clones do not contain stop codon at 3'end.
Translation: MAGFSRPNNSLLLKMPATLASSHTPNLSSLLFYDQNHGQAVNANAAAMMVEDDASLQSSSAVVDTSPQPQGSAAAASPME
RKRKPAEDSATLSSAQSKEDCKQQQESKSRRGKRPHNKEANTEEKSATEDEATRGYIHVRARRGQATDSHSLAERVRRER
ISERMRMLQALVPGCDKVTGKALILDEIINYVQSLQNQVEFLSMRIASMSPVLYGFGLDSDGLHDHAQKMGVMFQEALAM
AGPVLSQASPAAPSEAIMDTTTPYSLHGHGQGSISFSQDNGSSTYLMQHQQAAGEPTRQQELLSQLVFNNMCSFQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map