TFome Information for pUT3404

TFome Name:pUT3404
Genbank Insert ID:KJ726863
Protein Name:ZmbHLH137
Species: Maize
TF Family: bHLH
Template: BT036552
Corresponding TF Gene: GRMZM2G019806
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3271up 3' Primer Name: UT3271dn
5' Primer Sequence: caccATGGCAACGCAGTGGTTCTCCA 3' Primer Sequence: GAAGGATGATTTGAACGCTTGATTTAGC
5' Primer Tm (º C): 65.1 3' Primer Tm (º C): 56.8
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57.8°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGCAACGCAGTGGTTCTCCAACATGGTGATGGACGAGCCGAGCTTTTTCCACCAGTGGCAGTCCGACACGCTGCTGGA
GCAGTACACGGAGCAGCAGATCGCGGTGGCGTTCGGCCAGGGGGAGCCGGTGGACCACGCCGTGGCGGCGGCCCTGGCGA
CGGCGATGCCCATGCAGCTGCAGCAGCCCGCGGCGGCCGAGCAGCCGCACCACCGCCCGCGCAAGGCGGCCAAGGTCAAC
ACCAGCTGGGACTCGTGCATCACGGAGCAGGGCTCCCCCGCCGCGGACTCCTCCTCGCCGACCATCCTCTCCTTCGGCGG
CCACACCGCGTTCGCCAAGGCGGAGCCGACGACGCACCAGCCGCCCAGCTGCGCCGGGTACTACGGCGCCGCCGCCGCCG
CCGCGAAGGCGCCGAAGCAGGAGATGATGGACGCCGCCGCGATGCCGCCGTTCCAGCAGGCGCGCCCGGCCAAGCGTTCC
TACGACGACATGGCCGCGGTGGCCGAGGCCGCCAACGCGCCGTCCGCCAACACGCGCCCGGCCTCCCAGAACCAGGACCA
CATCTTGGCCGAGCGGAAGCGCCGCGAGAAGCTCAGCCAGCGCTTCATCGCCCTCTCCAAGATCGTCCCTGGCCTCAAGA
AGATGGACAAGGCATCGGTGCTCGGCGACGCGATCAAGTACGTGAAGCAGCTGCAGGACCAGGTGAAGGGGCTGGAGGAC
GACGCCCGGCGGCGCCCGGTCGAGGCGGCGGTGCTGGTCAAGAAGTCCCAGCTGTCGGCCGACGACGACGAGGGCTCCTC
CTGCGACGAGAACTTCGTTGCCACCGAGGCTTCCGGCACTCTGCCGGAGATCGAGGCCCGGGTGTCGGACCGCACAGTGC
TGGTCAGGATCCACTGCGAGAACCGCAAGGGGGTGCTCATCGCCGCCCTCTCTGAGGTCGAGCGCCTTGGCCTCTCCATC
ATGAACACCAATGTGCTCCCCTTCACAGCCTCCTCCCTCGACATCACCATCATGGCCATGGCTGGTGACGACTTCTGCTG
GTCTGTCAAGGATATTGTCAAGAAGCTAAATCAAGCGTTCAAATCATCCTTC
TFome clones do not contain stop codon at 3'end.
Translation: MATQWFSNMVMDEPSFFHQWQSDTLLEQYTEQQIAVAFGQGEPVDHAVAAALATAMPMQLQQPAAAEQPHHRPRKAAKVN
TSWDSCITEQGSPAADSSSPTILSFGGHTAFAKAEPTTHQPPSCAGYYGAAAAAAKAPKQEMMDAAAMPPFQQARPAKRS
YDDMAAVAEAANAPSANTRPASQNQDHILAERKRREKLSQRFIALSKIVPGLKKMDKASVLGDAIKYVKQLQDQVKGLED
DARRRPVEAAVLVKKSQLSADDDEGSSCDENFVATEASGTLPEIEARVSDRTVLVRIHCENRKGVLIAALSEVERLGLSI
MNTNVLPFTASSLDITIMAMAGDDFCWSVKDIVKKLNQAFKSSF
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map