TFome Information for pUT3406

TFome Name:pUT3406
Genbank Insert ID:KJ726865
Protein Name:ZmbHLH24
Species: Maize
TF Family: bHLH
Template: BT036901
Corresponding TF Gene: GRMZM2G085751
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: UT3275up 3' Primer Name: UT3275dn
5' Primer Sequence: caccATGGAAGACGGCGGCATGTG 3' Primer Sequence: CAATAACGTCTGCTGAATTTCCTGCA
5' Primer Tm (º C): 64.6 3' Primer Tm (º C): 57.9
PCR Condition: Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 57.8°C 30 sec anneal, 72°C 20 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (New England Biolabs),1XGC buffer (NEB), 3.5 mM MgCl2,10% Glycerol,40pg/ul template.
Nucleotide Sequence: ATGGAAGACGGCGGCATGTGCGAGCTCGTGATCGGCGACCACCGCAACCTCCGGCACCGCCTGTCGGGAGCCGAGGACCT
CTTCAGCATCGTCGGGACGTGGGAGGAGCGCACGAACGGCGCCAGTGGTGGTGGTGGTGGTGGAGGTGGTTCTTCTGCGG
CTGCTGTCCGAGCATATAGCCAGGGCTGCACTGCTGGCACCGCGGGAGCTAAAACGGCGGCTGGGACCAACAACAGCCGG
CGGCGCACGGGCGACGAGGAGAAAGGCGGCAGCGCTCCCGCGCAGAAGAAGCACAAGGGTTCGTCAGCGGTGTCCGATGA
CGAGGGAGCGGCGAAGATGTCACACATCACGGTGGAGCGCAACCGGAGGAAGCAGATGAACGAGCACCTCGCCGTGCTGC
GCTCGCTCATGCCCTGCTTCTACGTCAAAAGGGGTGACCAAGCATCCATCATAGGCGGGGTGGTGGACTACATCAAGGAG
CTTCAGCAGGTGCTGCGTTCGCTGGAGACGAAGAAGCACCGCAAGGCGTACGCGGAGCAGGTGCTGAGCCCGCGGCCTCT
CCCAGCAGTCAAGTCCACGCCGCCGCTCAGCCCGCACGTCGCGGTGCCCATGAGCCCCCGGACGCCGACCCCGGGCAGCC
CGTACAAGCCCGCATCCGGCGCGGCGGCGACGACGACTGGAAGCTGCAGACTCCCACACCGGGCCGCCGCCGCCGCCGCC
GCCTACATCGGAACGCCGACGACGTCGTCCTCCTCGTCGTCCTACTCGCACGATCAGCAGCGGCACTACTCGACGTACCT
GCCGACCCTAGACAGCCTCGTGACCGAGCTCGCCGCGCAGGCAGCAGCCTGCAGCAGGCCGGCGGCGTCCGGCGGGCTCA
CCCGGCTCCCCGACGTGAAGGTGGAGTTTGCAGGGCCCAACCTCGTGCTGAAGACGGTGTCTCACCGCTCGCCCGGGCAG
GCGCTCAAGATCATCGCAGCGCTCGAGAGCCTCCCGCTGGAGATCCTTCACGTCAGCGTCAGCACCGTGGACGACACCAT
GGTGCACTCCTTCACCATCAAGATTGGGATCGAGTGCGAGCTCAGCGCGGAGGAGCTTGTGCAGGAAATTCAGCAGACGT
TATTG
TFome clones do not contain stop codon at 3'end.
Translation: MEDGGMCELVIGDHRNLRHRLSGAEDLFSIVGTWEERTNGASGGGGGGGGSSAAAVRAYSQGCTAGTAGAKTAAGTNNSR
RRTGDEEKGGSAPAQKKHKGSSAVSDDEGAAKMSHITVERNRRKQMNEHLAVLRSLMPCFYVKRGDQASIIGGVVDYIKE
LQQVLRSLETKKHRKAYAEQVLSPRPLPAVKSTPPLSPHVAVPMSPRTPTPGSPYKPASGAAATTTGSCRLPHRAAAAAA
AYIGTPTTSSSSSSYSHDQQRHYSTYLPTLDSLVTELAAQAAACSRPAASGGLTRLPDVKVEFAGPNLVLKTVSHRSPGQ
ALKIIAALESLPLEILHVSVSTVDDTMVHSFTIKIGIECELSAEELVQEIQQTLL
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map